Albizia harveyi: phytochemical profiling, antioxidant, antidiabetic and hepatoprotective activities of the bark extract
Profiling the polyphenols in the methanol extract from the bark of Albizia harveyi was performed by HPLC-PDA-ESI-MS/MS analysis. The phytochemical analysis identified 39 compounds, the majority of them were flavan-3-ol derivatives and condensed tannins. Total phenolic content, determined by the Foli...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2017
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| In: |
Medicinal chemistry research
Year: 2017, Volume: 26, Issue: 12, Pages: 3091-3105 |
| ISSN: | 1554-8120 |
| DOI: | 10.1007/s00044-017-2005-8 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1007/s00044-017-2005-8 Verlag, Volltext: https://link.springer.com/article/10.1007/s00044-017-2005-8 
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| Author Notes: | Mansour Sobeh, Mona F. Mahmoud, Mohamed A. O. Abdelfattah, Hesham A. El-Beshbishy, Assem M. El-Shazly, Michael Wink |
| Summary: | Profiling the polyphenols in the methanol extract from the bark of Albizia harveyi was performed by HPLC-PDA-ESI-MS/MS analysis. The phytochemical analysis identified 39 compounds, the majority of them were flavan-3-ol derivatives and condensed tannins. Total phenolic content, determined by the Folin-Ciocalteu method amounted to 489 mg gallic acid equivalents/g extract. The extract showed promising antioxidant activities with an EC50 of 3.6 µg/mL and 18.32 mM FeSO4 equivalent/mg extract in radical scavenging assay and ferric reducing antioxidant power assays, respectively. The hepatoprotective potential of the extract in rats was determined in vivo in a d-galactosamine-induced liver toxicity model. A dose of 100 mg/kg (body weight) of the bark extract reduced levels of aspartate aminotransferase, gamma-glutamyltransferase and total bilirubin by 35.7, 65.3, and 23.8% (p < 0.05), respectively whereas glutathione was increased by 59.1%. These effects were similar to silymarin which was used as positive control. The extract (100 mg/kg (body weight) mediated a substantial antidiabetic response in streptozotocin-induced diabetic rats manifested by a significant reduction in serum glucose and lipid peroxides and significant increase of serum insulin. Docking of d-(+) catechin and the dimer (epi)catechin-(epi)catechin into the active site of the enzymes human pancreatic α-amylase, maltase-glucoamylase, and aldol reductase revealed that these enzymes may be possible targets via which, the studied Albizia harveyi extract could exert its antidiabetic effect. |
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| Item Description: | Gesehen am 14.02.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1554-8120 |
| DOI: | 10.1007/s00044-017-2005-8 |