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Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors

Somatic cell cytokinesis was shown to involve the insertion of sphingolipids (SLs) to midbodies prior to abscission. Spermatogenic midbodies transform into stable intercellular bridges (ICBs) connecting clonal daughter cells in a syncytium. This process requires specialized SL structures. (1) Using...

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Hauptverfasser: Rabionet Roig, Mariona (VerfasserIn) , Porubský, Štefan (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 04 June 2015
In: Human molecular genetics
Year: 2015, Jahrgang: 24, Heft: 17, Pages: 4792-4808
ISSN:1460-2083
DOI:10.1093/hmg/ddv204
Online-Zugang:Verlag, teilw. kostenfrei, Volltext: http://dx.doi.org/10.1093/hmg/ddv204
Verlag, teilw. kostenfrei, Volltext: https://academic-oup-com.ezproxy.medma.uni-heidelberg.de/hmg/article/24/17/4792/647747
Volltext
Verfasserangaben:Mariona Rabionet, Aline Bayerle, Richard Jennemann, Hans Heid, Jens Fuchser, Christian Marsching, Stefan Porubsky, Christian Bolenz, Florian Guillou, Hermann-Josef Gröne, Karin Gorgas and Roger Sandhoff
Beschreibung
Zusammenfassung:Somatic cell cytokinesis was shown to involve the insertion of sphingolipids (SLs) to midbodies prior to abscission. Spermatogenic midbodies transform into stable intercellular bridges (ICBs) connecting clonal daughter cells in a syncytium. This process requires specialized SL structures. (1) Using high resolution-mass spectrometric imaging, we show in situ a biphasic pattern of SL synthesis with testis-specific anchors. This pattern correlates with and depends on ceramide synthase 3 (CerS3) localization in both, pachytene spermatocytes until completion of meiosis and elongating spermatids. (2) Blocking the pathways to germ cell-specific ceramides (CerS3-KO) and further to glycosphingolipids (glucosylceramide synthase-KO) in mice highlights the need for special SLs for spermatid ICB stability. In contrast to somatic mitosis these SLs require ultra-long polyunsaturated anchors with unique physico-chemical properties, which can only be provided by CerS3. Loss of these anchors causes enhanced apoptosis during meiosis, formation of multinuclear giant cells and spermatogenic arrest. Hence, testis-specific SLs, which we also link to CerS3 in human testis, are quintessential for male fertility.
Beschreibung:Gesehen am 07.03.2018
Beschreibung:Online Resource
ISSN:1460-2083
DOI:10.1093/hmg/ddv204

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