Buchumschlag

Junb controls lymphatic vascular development in zebrafish via miR-182

JUNB, a subunit of the AP-1 transcription factor complex, mediates gene regulation in response to a plethora of extracellular stimuli. Previously, JUNB was shown to act as a critical positive regulator of blood vessel development and homeostasis as well as a negative regulator of proliferation, infl...

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Bibliographische Detailangaben
Hauptverfasser: Kiesow, Kristin (VerfasserIn) , Bennewitz, Katrin (VerfasserIn) , Cramer-Stoll, Sandra (VerfasserIn) , Kroll, Jens (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 13 October 2015
In: Scientific reports
Year: 2015, Jahrgang: 5
ISSN:2045-2322
DOI:10.1038/srep15007
Online-Zugang:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1038/srep15007
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/srep15007
Volltext
Verfasserangaben:Kristin Kiesow, Katrin Bennewitz, Laura Gutierrez Miranda, Sandra J. Stoll, Bettina Hartenstein, Peter Angel, Jens Kroll, Marina Schorpp-Kistner
Beschreibung
Zusammenfassung:JUNB, a subunit of the AP-1 transcription factor complex, mediates gene regulation in response to a plethora of extracellular stimuli. Previously, JUNB was shown to act as a critical positive regulator of blood vessel development and homeostasis as well as a negative regulator of proliferation, inflammation and tumour growth. Here, we demonstrate that the oncogenic miR-182 is a novel JUNB target. Loss-of-function studies by morpholino-mediated knockdown and the CRISPR/Cas9 technology identify a novel function for both JUNB and its target miR-182 in lymphatic vascular development in zebrafish. Furthermore, we show that miR-182 attenuates foxo1 expression indicating that strictly balanced Foxo1 levels are required for proper lymphatic vascular development in zebrafish. In conclusion, our findings uncover with the Junb/miR-182/Foxo1 regulatory axis a novel key player in governing lymphatic vascular morphogenesis in zebrafish.
Beschreibung:Gesehen am 15.03.2018
Beschreibung:Online Resource
ISSN:2045-2322
DOI:10.1038/srep15007

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