Functional analysis of recombinant channels in host cells using a fast agonist application system
A reduced recombinant system provides a unique opportunity to study the biophysical properties of NMDAR channels with known subunit compositions, by using a point mutation approach to analyze the structural determinants of receptor function (Wollmuth and Sobolevsky, Trends Neurosci 27:321-328, 2004)...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) Kapitel/Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
2017
|
| In: |
NMDA Receptors
Year: 2017, Pages: 163-169 |
| DOI: | 10.1007/978-1-4939-7321-7_8 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1007/978-1-4939-7321-7_8 |
| Verfasserangaben: | Andrei Rozov, David Jappy |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1572527129 | ||
| 003 | DE-627 | ||
| 005 | 20220814130421.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180502s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/978-1-4939-7321-7_8 |2 doi | |
| 035 | |a (DE-627)1572527129 | ||
| 035 | |a (DE-576)502527129 | ||
| 035 | |a (DE-599)BSZ502527129 | ||
| 035 | |a (OCoLC)1341008091 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Rozov, Andrei |d 1968- |e VerfasserIn |0 (DE-588)122769686 |0 (DE-627)706036190 |0 (DE-576)293413312 |4 aut | |
| 245 | 1 | 0 | |a Functional analysis of recombinant channels in host cells using a fast agonist application system |c Andrei Rozov, David Jappy |
| 264 | 1 | |c 2017 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 02.05.2018 | ||
| 520 | |a A reduced recombinant system provides a unique opportunity to study the biophysical properties of NMDAR channels with known subunit compositions, by using a point mutation approach to analyze the structural determinants of receptor function (Wollmuth and Sobolevsky, Trends Neurosci 27:321-328, 2004). However, in addition to the well-developed repertoire of molecular biological techniques, these types of studies also require electrophysiological methods that allow a wide range of receptor activation protocols that can adequately assess desensitization, inactivation, ion permeability, and other properties of the channels. Currently, one of the most well-developed techniques suitable for addressing these issues is use of the fast agonist application system for rapid activation of ligand gated ion-channels (Colquhoun et al., J Physiol 458:261-287, 1992; Jonas and Sakmann, J Physiol 455:143-171, 1992). | ||
| 700 | 1 | |a Jappy, David |e VerfasserIn |0 (DE-588)1156952530 |0 (DE-627)1019894334 |0 (DE-576)502528028 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t NMDA Receptors |d New York, NY : Humana Press, 2017 |g (2017), Seite 163-169 |h Online-Ressource (XIII, 314 p. 77 illus., 46 illus. in color, online resource) |w (DE-627)1656832429 |w (DE-576)495033944 |z 9781493973217 |7 nnam |a Functional analysis of recombinant channels in host cells using a fast agonist application system |
| 773 | 1 | 8 | |g year:2017 |g pages:163-169 |g extent:7 |a Functional analysis of recombinant channels in host cells using a fast agonist application system |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/978-1-4939-7321-7_8 |x Verlag |x Resolving-System |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180502 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 122769686 |a Rozov, Andrei |m 122769686:Rozov, Andrei |d 50000 |d 53200 |e 50000PR122769686 |e 53200PR122769686 |k 0/50000/ |k 1/50000/53200/ | ||
| 999 | |a KXP-PPN1572527129 |e 3007757193 | ||
| BIB | |a Y | ||
| JSO | |a {"person":[{"family":"Rozov","given":"Andrei","roleDisplay":"VerfasserIn","display":"Rozov, Andrei","role":"aut"},{"family":"Jappy","given":"David","display":"Jappy, David","roleDisplay":"VerfasserIn","role":"aut"}],"title":[{"title":"Functional analysis of recombinant channels in host cells using a fast agonist application system","title_sort":"Functional analysis of recombinant channels in host cells using a fast agonist application system"}],"type":{"media":"Online-Ressource","bibl":"chapter"},"note":["Gesehen am 02.05.2018"],"recId":"1572527129","language":["eng"],"name":{"displayForm":["Andrei Rozov, David Jappy"]},"origin":[{"dateIssuedKey":"2017","dateIssuedDisp":"2017"}],"id":{"doi":["10.1007/978-1-4939-7321-7_8"],"eki":["1572527129"]},"physDesc":[{"extent":"7 S."}],"relHost":[{"part":{"text":"(2017), Seite 163-169","extent":"7","year":"2017","pages":"163-169"},"language":["eng"],"recId":"1656832429","disp":"Functional analysis of recombinant channels in host cells using a fast agonist application systemNMDA Receptors","type":{"media":"Online-Ressource","bibl":"edited-book"},"title":[{"title_sort":"NMDA Receptors","title":"NMDA Receptors","subtitle":"Methods and Protocols"}],"person":[{"given":"Nail","family":"Burnashev","role":"edt","display":"Burnashev, Nail","roleDisplay":"Hrsg."},{"family":"Szepetowski","given":"Pierre","display":"Szepetowski, Pierre","roleDisplay":"Hrsg.","role":"edt"}],"relMultPart":[{"recId":"599671882","language":["eng"],"type":{"bibl":"serial","media":"Online-Ressource"},"note":["Gesehen am 05.11.12"],"disp":"Methods in Molecular Biology","part":{"number_sort":["1677"],"number":["1677"]},"pubHistory":["1.1984 -"],"title":[{"title":"Methods in molecular biology","title_sort":"Methods in molecular biology"}],"physDesc":[{"extent":"Online-Ressource"}],"dispAlt":"Methods in molecular biology","id":{"issn":["1940-6029"],"zdb":["2493551-7"],"eki":["599671882"]},"origin":[{"dateIssuedDisp":"1984-","dateIssuedKey":"1984","publisher":"Humana Press ; [Springer]","publisherPlace":"Totowa, NJ ; [Heidelberg]"}]}],"physDesc":[{"extent":"Online-Ressource (XIII, 314 p. 77 illus., 46 illus. in color, online resource)"}],"id":{"doi":["10.1007/978-1-4939-7321-7"],"eki":["1656832429"],"isbn":["9781493973217"]},"origin":[{"publisherPlace":"New York, NY","dateIssuedDisp":"2017","dateIssuedKey":"2017","publisher":"Humana Press"}],"name":{"displayForm":["edited by Nail Burnashev, Pierre Szepetowski"]}}]} | ||
| SRT | |a ROZOVANDREFUNCTIONAL2017 | ||