Adipose derived stem cells protect skin flaps against ischemia-reperfusion injury
BackgroundAdvances in the treatment of ischemia- reperfusion injury have created an opportunity for plastic surgeons to apply these treatments to flaps and implanted tissues. We examined the capability of adipose derived stem cells (ADSCs) to protect tissue against IRI using an extended inferior epi...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
17 April 2012
|
| In: |
Stem cell reviews and reports
Year: 2012, Volume: 8, Issue: 3, Pages: 854-862 |
| ISSN: | 2629-3277 |
| DOI: | 10.1007/s12015-012-9368-5 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1007/s12015-012-9368-5 Verlag, Volltext: https://link.springer.com/article/10.1007/s12015-012-9368-5 
|
| Author Notes: | Matthias A. Reichenberger, Sina Heimer, Amelia Schaefer, Ulrike Lass, Martha Maria Gebhard, Günter Germann, Uwe Leimer, Eva Köllensperger, Wolf Mueller |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 157258016X | ||
| 003 | DE-627 | ||
| 005 | 20220814131041.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180503s2012 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s12015-012-9368-5 |2 doi | |
| 035 | |a (DE-627)157258016X | ||
| 035 | |a (DE-576)50258016X | ||
| 035 | |a (DE-599)BSZ50258016X | ||
| 035 | |a (OCoLC)1341008076 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Reichenberger, Matthias |e VerfasserIn |0 (DE-588)1070543772 |0 (DE-627)824002520 |0 (DE-576)430210531 |4 aut | |
| 245 | 1 | 0 | |a Adipose derived stem cells protect skin flaps against ischemia-reperfusion injury |c Matthias A. Reichenberger, Sina Heimer, Amelia Schaefer, Ulrike Lass, Martha Maria Gebhard, Günter Germann, Uwe Leimer, Eva Köllensperger, Wolf Mueller |
| 264 | 1 | |c 17 April 2012 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 03.05.2018 | ||
| 520 | |a BackgroundAdvances in the treatment of ischemia- reperfusion injury have created an opportunity for plastic surgeons to apply these treatments to flaps and implanted tissues. We examined the capability of adipose derived stem cells (ADSCs) to protect tissue against IRI using an extended inferior epigastric artery skin flap as a flap ischemia- reperfusion injury (IRI) model.MethodsADSCs were isolated from Lewis rats and cultured in vitro. Twenty- four rats were randomly divided into three groups. Group I was the sham group and did not undergo ischemic insult; rather, the flap was raised and immediately sutured back (non-ischemic control group). Group II (ischemia control) and group III (ADSCs treatment) underwent 3 h of ischemic insult. During reperfusion group III was treated by intravenous application of ADSCs and group II was left untreated. Five days postoperatively, flap survival and perfusion were assessed. Microvessel density was visualized by immunohistochemistry and semi- quantitative real-time polymerase chain reaction addressed differential gene expression.ResultsTreatment with ADSCs significantly increased flap survival (p < 0.001) and flap perfusion (p < 0.001) when compared to the control group II. Microvessel- density in ADSCs treated group was not significantly increased in any group. No significant differences showed the comparison of the experimental group III and the sham operated control group I. ADSCs treatment (Group III) was accompanied by a significantly enhanced expression of pro-angiogenic and pro-inflammatory genes.ConclusionOverall, our study demonstrates that ADSCs treatment significantly enhances skin flap survival in the aftermath of ischemia to an extent that almost equals surgical results without ischemia. This effect is accompanied with a pronounced and significant angiogenic response and an improved blood perfusion. | ||
| 700 | 1 | |a Heimer, Sina Franziska |d 1986- |e VerfasserIn |0 (DE-588)1029153876 |0 (DE-627)732530415 |0 (DE-576)376602872 |4 aut | |
| 700 | 1 | |a Schaefer, Amelia |d 1985- |e VerfasserIn |0 (DE-588)1063383595 |0 (DE-627)81234703X |0 (DE-576)422645915 |4 aut | |
| 700 | 1 | |a Gebhard, Martha-Maria |d 1948- |e VerfasserIn |0 (DE-588)1076763278 |0 (DE-627)835312879 |0 (DE-576)445612789 |4 aut | |
| 700 | 1 | |a Germann, Günter |d 1952- |e VerfasserIn |0 (DE-588)1145880673 |0 (DE-627)1006989218 |0 (DE-576)17218732X |4 aut | |
| 700 | 1 | |a Leimer, Uwe |d 1965- |e VerfasserIn |0 (DE-588)123653320 |0 (DE-627)706383494 |0 (DE-576)293809593 |4 aut | |
| 700 | 1 | |a Köllensperger, Eva |d 1977- |e VerfasserIn |0 (DE-588)134097548 |0 (DE-627)560468822 |0 (DE-576)300310544 |4 aut | |
| 700 | 1 | |a Müller, Wolf C. |d 1967- |e VerfasserIn |0 (DE-588)1075182743 |0 (DE-627)832987476 |0 (DE-576)34814184X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Stem cell reviews and reports |d New York, NY : Springer, 2009 |g 8(2012), 3, Seite 854-862 |h Online-Ressource |w (DE-627)600310051 |w (DE-600)2495579-6 |w (DE-576)33496783X |x 2629-3277 |7 nnas |a Adipose derived stem cells protect skin flaps against ischemia-reperfusion injury |
| 773 | 1 | 8 | |g volume:8 |g year:2012 |g number:3 |g pages:854-862 |g extent:9 |a Adipose derived stem cells protect skin flaps against ischemia-reperfusion injury |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s12015-012-9368-5 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://link.springer.com/article/10.1007/s12015-012-9368-5 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180503 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 1075182743 |a Müller, Wolf C. |m 1075182743:Müller, Wolf C. |d 910000 |d 912000 |e 910000PM1075182743 |e 912000PM1075182743 |k 0/910000/ |k 1/910000/912000/ |p 9 |y j | ||
| 998 | |g 134097548 |a Köllensperger, Eva |m 134097548:Köllensperger, Eva |p 8 | ||
| 998 | |g 123653320 |a Leimer, Uwe |m 123653320:Leimer, Uwe |p 7 | ||
| 998 | |g 1145880673 |a Germann, Günter |m 1145880673:Germann, Günter |d 910000 |d 910200 |e 910000PG1145880673 |e 910200PG1145880673 |k 0/910000/ |k 1/910000/910200/ |p 6 | ||
| 998 | |g 1076763278 |a Gebhard, Martha-Maria |m 1076763278:Gebhard, Martha-Maria |d 910000 |d 910200 |e 910000PG1076763278 |e 910200PG1076763278 |k 0/910000/ |k 1/910000/910200/ |p 5 | ||
| 998 | |g 1063383595 |a Schaefer, Amelia |m 1063383595:Schaefer, Amelia |d 910000 |d 910200 |e 910000PS1063383595 |e 910200PS1063383595 |k 0/910000/ |k 1/910000/910200/ |p 3 | ||
| 998 | |g 1029153876 |a Heimer, Sina Franziska |m 1029153876:Heimer, Sina Franziska |d 910000 |d 910200 |e 910000PH1029153876 |e 910200PH1029153876 |k 0/910000/ |k 1/910000/910200/ | ||
| 998 | |g 1070543772 |a Reichenberger, Matthias |m 1070543772:Reichenberger, Matthias |d 910000 |d 910200 |e 910000PR1070543772 |e 910200PR1070543772 |k 0/910000/ |k 1/910000/910200/ |p 1 |x j | ||
| 999 | |a KXP-PPN157258016X |e 3007825709 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 03.05.2018"],"language":["eng"],"recId":"157258016X","title":[{"title":"Adipose derived stem cells protect skin flaps against ischemia-reperfusion injury","title_sort":"Adipose derived stem cells protect skin flaps against ischemia-reperfusion injury"}],"person":[{"roleDisplay":"VerfasserIn","display":"Reichenberger, Matthias","role":"aut","family":"Reichenberger","given":"Matthias"},{"display":"Heimer, Sina Franziska","roleDisplay":"VerfasserIn","role":"aut","family":"Heimer","given":"Sina Franziska"},{"role":"aut","display":"Schaefer, Amelia","roleDisplay":"VerfasserIn","given":"Amelia","family":"Schaefer"},{"family":"Gebhard","given":"Martha-Maria","display":"Gebhard, Martha-Maria","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Germann","given":"Günter","display":"Germann, Günter","roleDisplay":"VerfasserIn","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Leimer, Uwe","role":"aut","family":"Leimer","given":"Uwe"},{"roleDisplay":"VerfasserIn","display":"Köllensperger, Eva","role":"aut","family":"Köllensperger","given":"Eva"},{"role":"aut","display":"Müller, Wolf C.","roleDisplay":"VerfasserIn","given":"Wolf C.","family":"Müller"}],"physDesc":[{"extent":"9 S."}],"relHost":[{"title":[{"title":"Stem cell reviews and reports","title_sort":"Stem cell reviews and reports"}],"pubHistory":["5.2009 -"],"part":{"year":"2012","issue":"3","pages":"854-862","volume":"8","text":"8(2012), 3, Seite 854-862","extent":"9"},"type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 24.01.14"],"disp":"Adipose derived stem cells protect skin flaps against ischemia-reperfusion injuryStem cell reviews and reports","language":["eng"],"recId":"600310051","origin":[{"dateIssuedKey":"2009","publisher":"Springer ; Humana Press","dateIssuedDisp":"2009-","publisherPlace":"New York, NY ; New York, NY"}],"id":{"eki":["600310051"],"zdb":["2495579-6"],"issn":["2629-3277"]},"physDesc":[{"extent":"Online-Ressource"}]}],"origin":[{"dateIssuedKey":"2012","dateIssuedDisp":"17 April 2012"}],"id":{"doi":["10.1007/s12015-012-9368-5"],"eki":["157258016X"]},"name":{"displayForm":["Matthias A. Reichenberger, Sina Heimer, Amelia Schaefer, Ulrike Lass, Martha Maria Gebhard, Günter Germann, Uwe Leimer, Eva Köllensperger, Wolf Mueller"]}} | ||
| SRT | |a REICHENBERADIPOSEDER1720 | ||