Quantitative single-molecule localization microscopy (qSMLM) of membrane proteins based on kinetic analysis of fluorophore blinking cycles
Photoswitchable or photoactivatable fluorophores are the key in single-molecule localization microscopy. Next to providing fluorescence images with subdiffraction spatial resolution, additional information is available from observing single fluorophores over time. This includes the characteristic ph...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Dokumenttyp: | Kapitel/Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
2017
|
| In: |
Super-resolution microscopy
Year: 2017, Pages: 115-126 |
| Online-Zugang: | Verlag, Volltext: https://link.springer.com/protocol/10.1007/978-1-4939-7265-4_10
|
| Verfasserangaben: | Franziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, Mike Heilemann |
MARC
| LEADER | 00000caa a22000002c 4500 | ||
|---|---|---|---|
| 001 | 1574266632 | ||
| 003 | DE-627 | ||
| 005 | 20230427042853.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180508s2017 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)1574266632 | ||
| 035 | |a (DE-576)504266632 | ||
| 035 | |a (DE-599)BSZ504266632 | ||
| 035 | |a (OCoLC)1341009408 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Fricke, Franziska |d 1987- |e VerfasserIn |0 (DE-588)1083268767 |0 (DE-627)847913546 |0 (DE-576)455653755 |4 aut | |
| 245 | 1 | 0 | |a Quantitative single-molecule localization microscopy (qSMLM) of membrane proteins based on kinetic analysis of fluorophore blinking cycles |c Franziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, Mike Heilemann |
| 264 | 1 | |c 2017 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.05.2018 | ||
| 520 | |a Photoswitchable or photoactivatable fluorophores are the key in single-molecule localization microscopy. Next to providing fluorescence images with subdiffraction spatial resolution, additional information is available from observing single fluorophores over time. This includes the characteristic photophysical phenomenon of “blinking” that is exhibited by single fluorescent proteins or fluorophores and follows well-defined kinetic laws. Analyzing the kinetics of “blinking” allows determining the number of fluorophores in a multi-molecular complex. As such, quantitative information at the molecular level can be extracted, representing a tremendously useful extension of single-molecule super-resolution microscopy. This concept is in particular useful to study homo- and heterooligomeric signaling protein complexes in the plasma membrane of an intact cell with molecular resolution. Here, we provide an experimental framework for deciphering the stoichiometry of membrane proteins on the basis of SMLM and photoswitching statistics. | ||
| 700 | 1 | |a Beaudouin, Joël |e VerfasserIn |0 (DE-588)1085146421 |0 (DE-627)848832736 |0 (DE-576)45688811X |4 aut | |
| 700 | 1 | |a Eils, Roland |d 1965- |e VerfasserIn |0 (DE-588)1020648287 |0 (DE-627)691291705 |0 (DE-576)361718195 |4 aut | |
| 700 | 1 | |a Heilemann, Mike |e VerfasserIn |0 (DE-588)1096549891 |0 (DE-627)856929999 |0 (DE-576)33572535X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Super-resolution microscopy |d New York, NY : Humana Press, 2017 |g (2017), Seite 115-126 |h Online-Ressource (XI, 262 p. 48 illus., 37 illus. in color, online resource) |w (DE-627)1656117215 |w (DE-576)494007494 |z 9781493972654 |7 nnam |a Quantitative single-molecule localization microscopy (qSMLM) of membrane proteins based on kinetic analysis of fluorophore blinking cycles |
| 773 | 1 | 8 | |g year:2017 |g pages:115-126 |g extent:12 |a Quantitative single-molecule localization microscopy (qSMLM) of membrane proteins based on kinetic analysis of fluorophore blinking cycles |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |a Fricke, Franziska, 1987 - |t Quantitative single-molecule localization microscopy (qSMLM) of membrane proteins based on kinetic analysis of fluorophore blinking cycles |d 2017 |w (DE-627)157426740X |w (DE-576)50426740X |
| 856 | 4 | 0 | |u https://link.springer.com/protocol/10.1007/978-1-4939-7265-4_10 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180508 | ||
| 993 | |a BookComponentPart | ||
| 994 | |a 2017 | ||
| 998 | |g 1096549891 |a Heilemann, Mike |m 1096549891:Heilemann, Mike |d 700000 |d 716000 |d 700000 |d 718000 |e 700000PH1096549891 |e 716000PH1096549891 |e 700000PH1096549891 |e 718000PH1096549891 |k 0/700000/ |k 1/700000/716000/ |k 0/700000/ |k 1/700000/718000/ |p 5 |y j | ||
| 998 | |g 1020648287 |a Eils, Roland |m 1020648287:Eils, Roland |d 160000 |d 160100 |d 700000 |d 716000 |d 700000 |d 718000 |e 160000PE1020648287 |e 160100PE1020648287 |e 700000PE1020648287 |e 716000PE1020648287 |e 700000PE1020648287 |e 718000PE1020648287 |k 0/160000/ |k 1/160000/160100/ |k 0/700000/ |k 1/700000/716000/ |k 0/700000/ |k 1/700000/718000/ |p 4 | ||
| 998 | |g 1085146421 |a Beaudouin, Joël |m 1085146421:Beaudouin, Joël |p 2 | ||
| 999 | |a KXP-PPN1574266632 |e 3008526845 | ||
| BIB | |a Y | ||
| JSO | |a {"person":[{"family":"Fricke","given":"Franziska","roleDisplay":"VerfasserIn","display":"Fricke, Franziska","role":"aut"},{"role":"aut","display":"Beaudouin, Joël","roleDisplay":"VerfasserIn","given":"Joël","family":"Beaudouin"},{"role":"aut","display":"Eils, Roland","roleDisplay":"VerfasserIn","given":"Roland","family":"Eils"},{"family":"Heilemann","given":"Mike","roleDisplay":"VerfasserIn","display":"Heilemann, Mike","role":"aut"}],"title":[{"title_sort":"Quantitative single-molecule localization microscopy (qSMLM) of membrane proteins based on kinetic analysis of fluorophore blinking cycles","title":"Quantitative single-molecule localization microscopy (qSMLM) of membrane proteins based on kinetic analysis of fluorophore blinking cycles"}],"note":["Gesehen am 08.05.2018"],"type":{"bibl":"chapter","media":"Online-Ressource"},"recId":"1574266632","language":["eng"],"name":{"displayForm":["Franziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, Mike Heilemann"]},"origin":[{"dateIssuedKey":"2017","dateIssuedDisp":"2017"}],"id":{"eki":["1574266632"]},"physDesc":[{"extent":"12 S."}],"relHost":[{"disp":"Quantitative single-molecule localization microscopy (qSMLM) of membrane proteins based on kinetic analysis of fluorophore blinking cyclesSuper-resolution microscopy","type":{"media":"Online-Ressource","bibl":"edited-book"},"language":["eng"],"recId":"1656117215","part":{"year":"2017","pages":"115-126","text":"(2017), Seite 115-126","extent":"12"},"person":[{"role":"edt","roleDisplay":"Hrsg.","display":"Erfle, Holger","given":"Holger","family":"Erfle"}],"title":[{"title_sort":"Super-resolution microscopy","subtitle":"methods and protocols","title":"Super-resolution microscopy"}],"physDesc":[{"extent":"Online-Ressource (XI, 262 p. 48 illus., 37 illus. in color, online resource)"}],"relMultPart":[{"id":{"zdb":["2493551-7"],"eki":["599671882"],"issn":["1940-6029"]},"origin":[{"publisherPlace":"Totowa, NJ ; [Heidelberg]","dateIssuedDisp":"1984-","publisher":"Humana Press ; [Springer]","dateIssuedKey":"1984"}],"dispAlt":"Methods in molecular biology","physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Methods in molecular biology","title":"Methods in molecular biology"}],"part":{"number":["1663"],"number_sort":["1663"]},"pubHistory":["1.1984 -"],"language":["eng"],"recId":"599671882","type":{"media":"Online-Ressource","bibl":"serial"},"note":["Gesehen am 05.11.12"],"disp":"Methods in Molecular Biology"}],"name":{"displayForm":["edited by Holger Erfle"]},"origin":[{"dateIssuedDisp":"2017","publisher":"Humana Press","dateIssuedKey":"2017","publisherPlace":"New York, NY"}],"id":{"isbn":["9781493972654"],"eki":["1656117215"],"doi":["10.1007/978-1-4939-7265-4"]}}]} | ||
| SRT | |a FRICKEFRANQUANTITATI2017 | ||