Quantitative single-molecule localization microscopy (qSMLM) of membrane proteins based on kinetic analysis of fluorophore blinking cycles
Photoswitchable or photoactivatable fluorophores are the key in single-molecule localization microscopy. Next to providing fluorescence images with subdiffraction spatial resolution, additional information is available from observing single fluorophores over time. This includes the characteristic ph...
Gespeichert in:
| Hauptverfasser: | , , , |
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| Dokumenttyp: | Kapitel/Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
2017
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| In: |
Super-resolution microscopy
Year: 2017, Pages: 115-126 |
| Online-Zugang: | Verlag, Volltext: https://link.springer.com/protocol/10.1007/978-1-4939-7265-4_10
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| Verfasserangaben: | Franziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, Mike Heilemann |