Reconstitution of the complete pathway of ITS2 processing at the pre-ribosome
Excision of internal transcribed spacer 2 (ITS2) within eukaryotic pre-ribosomal RNA is essential for ribosome function. Here, the authors reconstitute the entire cycle of ITS2 processing in vitro using purified components, providing insights into the cleavage process and demonstrating that 26S pre-...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
27 November 2017
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| In: |
Nature Communications
Year: 2017, Volume: 8 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-017-01786-9 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1038/s41467-017-01786-9 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-017-01786-9 
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| Author Notes: | Lisa Fromm, Sebastian Falk, Dirk Flemming, Jan Michael Schuller, Matthias Thoms, Elena Conti & Ed Hurt |
| Summary: | Excision of internal transcribed spacer 2 (ITS2) within eukaryotic pre-ribosomal RNA is essential for ribosome function. Here, the authors reconstitute the entire cycle of ITS2 processing in vitro using purified components, providing insights into the cleavage process and demonstrating that 26S pre-rRNA processing necessarily precedes 7S pre-rRNA processing. |
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| Item Description: | Gesehen am 28.05.2018 |
| Physical Description: | Online Resource |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-017-01786-9 |