In vivo RNAi in the drosophila follicular epithelium: analysis of stem cell maintenance, proliferation, and differentiation
In vivo RNAi in Drosophila facilitates simple and rapid analysis of gene functions in a cell- or tissue-specific manner. The versatility of the UAS-GAL4 system allows to control exactly where and when during development the function of a gene is depleted. The epithelium of the ovary is a particularl...
Gespeichert in:
| 1. Verfasser: | |
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| Dokumenttyp: | Kapitel/Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
4 July 2017
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| In: |
RNAi and Small Regulatory RNAs in Stem Cells
Year: 2017, Pages: 185-206 |
| Online-Zugang: | Verlag, Volltext: https://link.springer.com/protocol/10.1007/978-1-4939-7108-4_14 Verlag, Volltext: http://www.springerprotocols.com/Pdf/doi/10.1007/978-1-4939-7108-4_14?encCode=QkVDOjQxXzQtODAxNy05Mzk0LTEtODc5&tokenString=nWllE+cnvzLdjQtzRSuXOQ==&access=denied 
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| Verfasserangaben: | Veit Riechmann |
MARC
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| 520 | |a In vivo RNAi in Drosophila facilitates simple and rapid analysis of gene functions in a cell- or tissue-specific manner. The versatility of the UAS-GAL4 system allows to control exactly where and when during development the function of a gene is depleted. The epithelium of the ovary is a particularly good model to study in a living animal how stem cells are maintained and how their descendants proliferate and differentiate. Here I provide basic information about the publicly available reagents for in vivo RNAi, and I describe how the oogenesis system can be applied to analyze stem cells and epithelial development at a histological level. Moreover, I give helpful hints to optimize the use of the UAS-GAL4 system for RNAi induction in the follicular epithelium. Finally, I provide detailed step-by-step protocols for ovary dissection, antibody stainings, and ovary mounting for microscopic analysis. | ||
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