Cover Image

Assessment of the multiplex PCR-based assay unyvero pneumonia application for detection of bacterial pathogens and antibiotic resistance genes in children and neonates

BackgroundPneumonia is a major healthcare problem. Rapid pathogen identification is critical, but often delayed due to the duration of culturing. Early, broad antibacterial therapy might lead to false-negative culture findings and eventually to the development of antibiotic resistances. We aimed to...

Full description

Saved in:
Bibliographic Details
Main Author: Papan, Cihan (Author)
Format: Article (Journal)
Language:English
Published: 2018
In: Infection
Year: 2017, Volume: 46, Issue: 2, Pages: 189-196
ISSN:1439-0973
DOI:10.1007/s15010-017-1088-y
Online Access:Verlag, Volltext: http://dx.doi.org/10.1007/s15010-017-1088-y
Verlag, Volltext: https://link.springer.com/article/10.1007/s15010-017-1088-y
Get full text
Author Notes:Cihan Papan, Melanie Meyer-Buehn, Gudrun Laniado, Thomas Nicolai, Matthias Griese, Johannes Huebner

MARC

LEADER 00000caa a2200000 c 4500
001 157613217X
003 DE-627
005 20220814154719.0
007 cr uuu---uuuuu
008 180607r20182017xx |||||o 00| ||eng c
024 7 |a 10.1007/s15010-017-1088-y  |2 doi 
035 |a (DE-627)157613217X 
035 |a (DE-576)50613217X 
035 |a (DE-599)BSZ50613217X 
035 |a (OCoLC)1341011106 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Papan, Cihan  |d 1984-  |e VerfasserIn  |0 (DE-588)1076370381  |0 (DE-627)834800187  |0 (DE-576)44524805X  |4 aut 
245 1 0 |a Assessment of the multiplex PCR-based assay unyvero pneumonia application for detection of bacterial pathogens and antibiotic resistance genes in children and neonates  |c Cihan Papan, Melanie Meyer-Buehn, Gudrun Laniado, Thomas Nicolai, Matthias Griese, Johannes Huebner 
264 1 |c 2018 
300 |a 8 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 07.06.2018 
500 |a Published online: 31 October 2017 
520 |a BackgroundPneumonia is a major healthcare problem. Rapid pathogen identification is critical, but often delayed due to the duration of culturing. Early, broad antibacterial therapy might lead to false-negative culture findings and eventually to the development of antibiotic resistances. We aimed to assess the accuracy of the new application Unyvero P50 based on multiplex PCR to detect bacterial pathogens in respiratory specimens from children and neonates.MethodsIn this prospective study, bronchoalveolar lavage fluids, tracheal aspirates, or pleural fluids from neonates and children were analyzed by both traditional culture methods and Unyvero multiplex PCR.ResultsWe analyzed specimens from 79 patients with a median age of 1.8 (range 0.01-20.1). Overall, Unyvero yielded a sensitivity of 73.1% and a specificity of 97.9% compared to culture methods. Best results were observed for non-fermenting bacteria, for which sensitivity of Unyvero was 90% and specificity 97.3%, while rates were lower for Gram-positive bacteria (46.2 and 93.9%, respectively). For resistance genes, we observed a concordance with antibiogram of 75% for those specimens in which there was a cultural correlate.ConclusionsUnyvero is a fast and easy-to-use tool that might provide additional information for clinical decision making, especially in neonates and in the setting of nosocomial pneumonia. Sensitivity of the PCR for Gram-positive bacteria and important resistance genes must be improved before this application can be widely recommended. 
534 |c 2017 
773 0 8 |i Enthalten in  |t Infection  |d München : Urban & Vogel, 1973  |g 46(2018), 2, Seite 189-196  |h Online-Ressource  |w (DE-627)31236945X  |w (DE-600)2006315-5  |w (DE-576)090889487  |x 1439-0973  |7 nnas  |a Assessment of the multiplex PCR-based assay unyvero pneumonia application for detection of bacterial pathogens and antibiotic resistance genes in children and neonates 
773 1 8 |g volume:46  |g year:2018  |g number:2  |g pages:189-196  |g extent:8  |a Assessment of the multiplex PCR-based assay unyvero pneumonia application for detection of bacterial pathogens and antibiotic resistance genes in children and neonates 
856 4 0 |u http://dx.doi.org/10.1007/s15010-017-1088-y  |x Verlag  |x Resolving-System  |3 Volltext 
856 4 0 |u https://link.springer.com/article/10.1007/s15010-017-1088-y  |x Verlag  |3 Volltext 
951 |a AR 
992 |a 20180607 
993 |a Article 
994 |a 2018 
998 |g 1076370381  |a Papan, Cihan  |m 1076370381:Papan, Cihan  |d 60000  |d 62300  |e 60000PP1076370381  |e 62300PP1076370381  |k 0/60000/  |k 1/60000/62300/  |p 1  |x j 
999 |a KXP-PPN157613217X  |e 301203403X 
BIB |a Y 
SER |a journal 
JSO |a {"note":["Gesehen am 07.06.2018","Published online: 31 October 2017"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"recId":"157613217X","language":["eng"],"title":[{"title":"Assessment of the multiplex PCR-based assay unyvero pneumonia application for detection of bacterial pathogens and antibiotic resistance genes in children and neonates","title_sort":"Assessment of the multiplex PCR-based assay unyvero pneumonia application for detection of bacterial pathogens and antibiotic resistance genes in children and neonates"}],"person":[{"role":"aut","display":"Papan, Cihan","roleDisplay":"VerfasserIn","given":"Cihan","family":"Papan"}],"physDesc":[{"extent":"8 S."}],"relHost":[{"title":[{"title":"Infection","title_sort":"Infection"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 05.12.05"],"disp":"Assessment of the multiplex PCR-based assay unyvero pneumonia application for detection of bacterial pathogens and antibiotic resistance genes in children and neonatesInfection","language":["eng"],"recId":"31236945X","pubHistory":["1.1973 -"],"part":{"issue":"2","pages":"189-196","year":"2018","extent":"8","text":"46(2018), 2, Seite 189-196","volume":"46"},"origin":[{"dateIssuedDisp":"1973-","dateIssuedKey":"1973","publisher":"Urban & Vogel ; Springer","publisherPlace":"München ; Berlin ; Heidelberg"}],"id":{"zdb":["2006315-5"],"eki":["31236945X"],"issn":["1439-0973"]},"physDesc":[{"extent":"Online-Ressource"}]}],"origin":[{"dateIssuedKey":"2018","dateIssuedDisp":"2018"}],"id":{"doi":["10.1007/s15010-017-1088-y"],"eki":["157613217X"]},"name":{"displayForm":["Cihan Papan, Melanie Meyer-Buehn, Gudrun Laniado, Thomas Nicolai, Matthias Griese, Johannes Huebner"]}} 
SRT |a PAPANCIHANASSESSMENT2018 

Similar Items