Quantification of femtomolar concentrations of the CYP3A substrate midazolam and its main metabolite 1′-hydroxymidazolam in human plasma using ultra performance liquid chromatography coupled to tandem mass spectrometry

The benzodiazepine midazolam is a probe drug used to phenotype cytochrome P450 3A activity. In this situation, effective sedative concentrations are neither needed nor desired, and in fact the use of very low doses is advantageous. We therefore developed and validated an assay for the femtomolar qua...

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Main Authors:	Burhenne, Jürgen (Author) , Halama, Birte (Author) , Maurer, Monika (Author) , Riedel, Klaus-Dieter (Author) , Hohmann, Nicolas (Author) , Mikus, Gerd (Author) , Haefeli, Walter E. (Author)
Format:	Article (Journal)
Language:	English
Published:	15 January 2012
In:	Analytical and bioanalytical chemistry Year: 2012, Volume: 402, Issue: 7, Pages: 2439-2450
ISSN:	1618-2650
DOI:	10.1007/s00216-011-5675-y
Online Access:	Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s00216-011-5675-y Verlag, Volltext: https://link.springer.com/article/10.1007/s00216-011-5675-y
Author Notes:	Jürgen Burhenne, Birte Halama, Monika Maurer, Klaus-Dieter Riedel, Nicolas Hohmann, Gerd Mikus, Walter E. Haefeli