Concerted action of the ribosome and the associated chaperone trigger factor confines nascent polypeptide folding
Summary: How nascent polypeptides emerging from ribosomes fold into functional structures is poorly understood. Here, we monitor disulfide bond formation, protease resistance, and enzymatic activity in nascent polypeptides to show that in close proximity to the ribosome, conformational space and kin...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2012
|
| In: |
Molecular cell
Year: 2012, Volume: 48, Issue: 1, Pages: 63-74 |
| ISSN: | 1097-4164 |
| DOI: | 10.1016/j.molcel.2012.07.018 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1016/j.molcel.2012.07.018 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S1097276512006454 
|
| Author Notes: | Anja Hoffmann, Annemarie H. Becker, Beate Zachmann-Brand, Elke Deuerling, Bernd Bukau, and Günter Kramer |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1576738361 | ||
| 003 | DE-627 | ||
| 005 | 20250206095712.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180621s2012 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.molcel.2012.07.018 |2 doi | |
| 035 | |a (DE-627)1576738361 | ||
| 035 | |a (DE-576)506738361 | ||
| 035 | |a (DE-599)BSZ506738361 | ||
| 035 | |a (OCoLC)1341012141 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Hoffmann, Anja |e VerfasserIn |0 (DE-588)137474415 |0 (DE-627)593173155 |0 (DE-576)303788577 |4 aut | |
| 245 | 1 | 0 | |a Concerted action of the ribosome and the associated chaperone trigger factor confines nascent polypeptide folding |c Anja Hoffmann, Annemarie H. Becker, Beate Zachmann-Brand, Elke Deuerling, Bernd Bukau, and Günter Kramer |
| 264 | 1 | |c 2012 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Available online 23 August 2012 | ||
| 500 | |a Gesehen am 21.06.2018 | ||
| 520 | |a Summary: How nascent polypeptides emerging from ribosomes fold into functional structures is poorly understood. Here, we monitor disulfide bond formation, protease resistance, and enzymatic activity in nascent polypeptides to show that in close proximity to the ribosome, conformational space and kinetics of folding are restricted. Folding constraints decrease incrementally with distance from the ribosome surface. Upon ribosome binding, the chaperone Trigger Factor counters folding also of longer nascent chains, to extents varying between different chain segments. Trigger Factor even binds and unfolds pre-existing folded structures, the unfolding activity being limited by the thermodynamic stability of nascent chains. Folding retardation and unfolding activities are not shared by the DnaK chaperone assisting later folding steps. These ribosome- and Trigger Factor-specific activities together constitute an efficient mechanism to prevent or even revert premature folding, effectively limiting misfolded intermediates during protein synthesis. | ||
| 700 | 1 | |a Becker, Annemarie |e VerfasserIn |0 (DE-588)1029442436 |0 (DE-627)733557635 |0 (DE-576)377295647 |4 aut | |
| 700 | 1 | |a Zachmann-Brand, Beate |e VerfasserIn |0 (DE-588)1098172795 |0 (DE-627)857465244 |0 (DE-576)469003944 |4 aut | |
| 700 | 1 | |a Bukau, Bernd |d 1954- |e VerfasserIn |0 (DE-588)143209019 |0 (DE-627)643892575 |0 (DE-576)335649270 |4 aut | |
| 700 | 1 | |a Kramer, Günter |e VerfasserIn |0 (DE-588)1060239582 |0 (DE-627)799340294 |0 (DE-576)416341136 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular cell |d [Cambridge, Mass.] : Cell Press, 1997 |g 48(2012), 1, Seite 63-74 |h Online-Ressource |w (DE-627)320416097 |w (DE-600)2001948-8 |w (DE-576)090881362 |x 1097-4164 |7 nnas |a Concerted action of the ribosome and the associated chaperone trigger factor confines nascent polypeptide folding |
| 773 | 1 | 8 | |g volume:48 |g year:2012 |g number:1 |g pages:63-74 |g extent:12 |a Concerted action of the ribosome and the associated chaperone trigger factor confines nascent polypeptide folding |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.molcel.2012.07.018 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S1097276512006454 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180621 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 1060239582 |a Kramer, Günter |m 1060239582:Kramer, Günter |d 700000 |d 706000 |e 700000PK1060239582 |e 706000PK1060239582 |k 0/700000/ |k 1/700000/706000/ |p 6 |y j | ||
| 998 | |g 143209019 |a Bukau, Bernd |m 143209019:Bukau, Bernd |d 700000 |d 706000 |d 700000 |d 718000 |e 700000PB143209019 |e 706000PB143209019 |e 700000PB143209019 |e 718000PB143209019 |k 0/700000/ |k 1/700000/706000/ |k 0/700000/ |k 1/700000/718000/ |p 5 | ||
| 998 | |g 1098172795 |a Zachmann-Brand, Beate |m 1098172795:Zachmann-Brand, Beate |d 700000 |d 706000 |e 700000PZ1098172795 |e 706000PZ1098172795 |k 0/700000/ |k 1/700000/706000/ |p 3 | ||
| 998 | |g 1029442436 |a Becker, Annemarie |m 1029442436:Becker, Annemarie |d 140000 |d 140001 |e 140000PB1029442436 |e 140001PB1029442436 |k 0/140000/ |k 1/140000/140001/ |p 2 | ||
| 998 | |g 137474415 |a Hoffmann, Anja |m 137474415:Hoffmann, Anja |d 700000 |d 706000 |e 700000PH137474415 |e 706000PH137474415 |k 0/700000/ |k 1/700000/706000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1576738361 |e 3013334193 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"type":{"media":"Online-Ressource","bibl":"article-journal"},"recId":"1576738361","id":{"eki":["1576738361"],"doi":["10.1016/j.molcel.2012.07.018"]},"name":{"displayForm":["Anja Hoffmann, Annemarie H. Becker, Beate Zachmann-Brand, Elke Deuerling, Bernd Bukau, and Günter Kramer"]},"origin":[{"dateIssuedDisp":"2012","dateIssuedKey":"2012"}],"language":["eng"],"note":["Available online 23 August 2012","Gesehen am 21.06.2018"],"person":[{"role":"aut","family":"Hoffmann","display":"Hoffmann, Anja","given":"Anja"},{"role":"aut","family":"Becker","display":"Becker, Annemarie","given":"Annemarie"},{"role":"aut","family":"Zachmann-Brand","display":"Zachmann-Brand, Beate","given":"Beate"},{"given":"Bernd","display":"Bukau, Bernd","family":"Bukau","role":"aut"},{"given":"Günter","display":"Kramer, Günter","role":"aut","family":"Kramer"}],"relHost":[{"title":[{"title":"Molecular cell","title_sort":"Molecular cell"}],"physDesc":[{"extent":"Online-Ressource"}],"part":{"extent":"12","issue":"1","pages":"63-74","year":"2012","volume":"48","text":"48(2012), 1, Seite 63-74"},"language":["eng"],"note":["Gesehen am 01.08.25"],"pubHistory":["1.1997/98 -"],"origin":[{"publisherPlace":"[Cambridge, Mass.] ; New York, NY","dateIssuedKey":"1997","dateIssuedDisp":"1997-","publisher":"Cell Press ; Elsevier"}],"disp":"Concerted action of the ribosome and the associated chaperone trigger factor confines nascent polypeptide foldingMolecular cell","type":{"media":"Online-Ressource","bibl":"periodical"},"id":{"issn":["1097-4164"],"eki":["320416097"],"zdb":["2001948-8"]},"recId":"320416097"}],"title":[{"title_sort":"Concerted action of the ribosome and the associated chaperone trigger factor confines nascent polypeptide folding","title":"Concerted action of the ribosome and the associated chaperone trigger factor confines nascent polypeptide folding"}],"physDesc":[{"extent":"12 S."}]} | ||
| SRT | |a HOFFMANNANCONCERTEDA2012 | ||