miR-16 and miR-125b are involved in barrier function dysregulation through the modulation of claudin-2 and cingulin expression in the jejunum in IBS with diarrhoea
Objective Micro-RNAs (miRNAs) play a crucial role in controlling intestinal epithelial barrier function partly by modulating the expression of tight junction (TJ) proteins. We have previously shown differential messenger RNA (mRNA) expression correlated with ultrastructural abnormalities of the epit...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2017
|
| In: |
Gut
Year: 2017, Jahrgang: 66, Heft: 9, Pages: 1537-1538 |
| ISSN: | 1468-3288 |
| DOI: | 10.1136/gutjnl-2016-311477 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1136/gutjnl-2016-311477 Verlag, Volltext: http://gut.bmj.com/content/66/9/1537.1 
|
| Verfasserangaben: | Cristina Martínez, Bruno K. Rodiño-Janeiro, Beatriz Lobo, Megan L. Stanifer, Bernd Klaus, Martin Granzow, Ana M. González-Castro, Eloisa Salvo-Romero, Carmen Alonso-Cotoner, Marc Pigrau, Ralph Roeth, Gudrun Rappold, Wolfgang Huber, Rosa González-Silos, Justo Lorenzo, Inés de Torres, Fernando Azpiroz, Steeve Boulant, María Vicario, Beate Niesler, Javier Santos |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1576839966 | ||
| 003 | DE-627 | ||
| 005 | 20220814174116.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180626s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1136/gutjnl-2016-311477 |2 doi | |
| 035 | |a (DE-627)1576839966 | ||
| 035 | |a (DE-576)506839966 | ||
| 035 | |a (DE-599)BSZ506839966 | ||
| 035 | |a (OCoLC)1341012339 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Martinez, Cristina |e VerfasserIn |0 (DE-588)1161443738 |0 (DE-627)1024748219 |0 (DE-576)506536459 |4 aut | |
| 245 | 1 | 0 | |a miR-16 and miR-125b are involved in barrier function dysregulation through the modulation of claudin-2 and cingulin expression in the jejunum in IBS with diarrhoea |c Cristina Martínez, Bruno K. Rodiño-Janeiro, Beatriz Lobo, Megan L. Stanifer, Bernd Klaus, Martin Granzow, Ana M. González-Castro, Eloisa Salvo-Romero, Carmen Alonso-Cotoner, Marc Pigrau, Ralph Roeth, Gudrun Rappold, Wolfgang Huber, Rosa González-Silos, Justo Lorenzo, Inés de Torres, Fernando Azpiroz, Steeve Boulant, María Vicario, Beate Niesler, Javier Santos |
| 264 | 1 | |c 2017 | |
| 300 | |a 2 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Published online first 12 January 2017 | ||
| 500 | |a Gesehen am 26.06.2018 | ||
| 520 | |a Objective Micro-RNAs (miRNAs) play a crucial role in controlling intestinal epithelial barrier function partly by modulating the expression of tight junction (TJ) proteins. We have previously shown differential messenger RNA (mRNA) expression correlated with ultrastructural abnormalities of the epithelial barrier in patients with diarrhoea-predominant IBS (IBS-D). However, the participation of miRNAs in these differential mRNA-associated findings remains to be established. Our aims were (1) to identify miRNAs differentially expressed in the small bowel mucosa of patients with IBS-D and (2) to explore putative target genes specifically involved in epithelial barrier function that are controlled by specific dysregulated IBS-D miRNAs. Design Healthy controls and patients meeting Rome III IBS-D criteria were studied. Intestinal tissue samples were analysed to identify potential candidates by: (a) miRNA-mRNA profiling; (b) miRNA-mRNA pairing analysis to assess the co-expression profile of miRNA-mRNA pairs; (c) pathway analysis and upstream regulator identification; (d) miRNA and target mRNA validation. Candidate miRNA-mRNA pairs were functionally assessed in intestinal epithelial cells. Results IBS-D samples showed distinct miRNA and mRNA profiles compared with healthy controls. TJ signalling was associated with the IBS-D transcriptional profile. Further validation of selected genes showed consistent upregulation in 75% of genes involved in epithelial barrier function. Bioinformatic analysis of putative miRNA binding sites identified hsa-miR-125b-5p and hsa-miR-16 as regulating expression of the TJ genes CGN (cingulin) and CLDN2 (claudin-2), respectively. Consistently, protein expression of CGN and CLDN2 was upregulated in IBS-D, while the respective targeting miRNAs were downregulated. In addition, bowel dysfunction, perceived stress and depression and number of mast cells correlated with the expression of hsa-miR-125b-5p and hsa-miR-16 and their respective target proteins. Conclusions Modulation of the intestinal epithelial barrier function in IBS-D involves both transcriptional and post-transcriptional mechanisms. These molecular mechanisms include miRNAs as master regulators in controlling the expression of TJ proteins and are associated with major clinical symptoms. | ||
| 650 | 4 | |a GENE EXPRESSION | |
| 650 | 4 | |a INTESTINAL BARRIER FUNCTION | |
| 650 | 4 | |a IRRITABLE BOWEL SYNDROME | |
| 650 | 4 | |a MOLECULAR BIOLOGY | |
| 650 | 4 | |a RNA EXPRESSION | |
| 700 | 1 | |a Stanifer, Megan |e VerfasserIn |0 (DE-588)1130687643 |0 (DE-627)884888835 |0 (DE-576)486922367 |4 aut | |
| 700 | 1 | |a Granzow, Martin |d 1970- |e VerfasserIn |0 (DE-588)121585298 |0 (DE-627)705555712 |0 (DE-576)292784929 |4 aut | |
| 700 | 1 | |a Röth, Ralph |e VerfasserIn |0 (DE-588)1068410221 |0 (DE-627)820235563 |0 (DE-576)427767059 |4 aut | |
| 700 | 1 | |a Rappold, Gudrun |d 1954- |e VerfasserIn |0 (DE-588)102028319X |0 (DE-627)691169594 |0 (DE-576)359985947 |4 aut | |
| 700 | 1 | |a Huber, Wolfgang |e VerfasserIn |0 (DE-588)1115277685 |0 (DE-627)870097997 |0 (DE-576)477724442 |4 aut | |
| 700 | 1 | |a González Silos, Rosa |e VerfasserIn |0 (DE-588)1121144195 |0 (DE-627)874011930 |0 (DE-576)480745471 |4 aut | |
| 700 | 1 | |a Lorenzo Bermejo, Justo |d 1972- |e VerfasserIn |0 (DE-588)124754619 |0 (DE-627)706705572 |0 (DE-576)294483632 |4 aut | |
| 700 | 1 | |a Boulant, Steeve |e VerfasserIn |0 (DE-588)1130687430 |0 (DE-627)884888509 |0 (DE-576)48692243X |4 aut | |
| 700 | 1 | |a Niesler, Beate |e VerfasserIn |0 (DE-588)1066800693 |0 (DE-627)817949615 |0 (DE-576)426148452 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Gut |d London : BMJ Publishing Group, 1960 |g 66(2017), 9, Seite 1537-1538 |h Online-Ressource |w (DE-627)302725628 |w (DE-600)1492637-4 |w (DE-576)082435405 |x 1468-3288 |7 nnas |a miR-16 and miR-125b are involved in barrier function dysregulation through the modulation of claudin-2 and cingulin expression in the jejunum in IBS with diarrhoea |
| 773 | 1 | 8 | |g volume:66 |g year:2017 |g number:9 |g pages:1537-1538 |g extent:2 |a miR-16 and miR-125b are involved in barrier function dysregulation through the modulation of claudin-2 and cingulin expression in the jejunum in IBS with diarrhoea |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1136/gutjnl-2016-311477 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://gut.bmj.com/content/66/9/1537.1 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180626 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 1066800693 |a Niesler, Beate |m 1066800693:Niesler, Beate |d 910000 |d 911500 |e 910000PN1066800693 |e 911500PN1066800693 |k 0/910000/ |k 1/910000/911500/ |p 20 | ||
| 998 | |g 1130687430 |a Boulant, Steeve |m 1130687430:Boulant, Steeve |d 700000 |d 718000 |e 700000PB1130687430 |e 718000PB1130687430 |k 0/700000/ |k 1/700000/718000/ |p 18 | ||
| 998 | |g 124754619 |a Lorenzo Bermejo, Justo |m 124754619:Lorenzo Bermejo, Justo |d 910000 |d 999701 |e 910000PL124754619 |e 999701PL124754619 |k 0/910000/ |k 1/910000/999701/ |p 15 | ||
| 998 | |g 1121144195 |a González Silos, Rosa |m 1121144195:González Silos, Rosa |d 910000 |d 999701 |e 910000PG1121144195 |e 999701PG1121144195 |k 0/910000/ |k 1/910000/999701/ |p 14 | ||
| 998 | |g 1115277685 |a Huber, Wolfgang |m 1115277685:Huber, Wolfgang |d 700000 |d 718000 |e 700000PH1115277685 |e 718000PH1115277685 |k 0/700000/ |k 1/700000/718000/ |p 13 | ||
| 998 | |g 102028319X |a Rappold, Gudrun |m 102028319X:Rappold, Gudrun |d 910000 |d 911500 |d 700000 |d 718000 |e 910000PR102028319X |e 911500PR102028319X |e 700000PR102028319X |e 718000PR102028319X |k 0/910000/ |k 1/910000/911500/ |k 0/700000/ |k 1/700000/718000/ |p 12 | ||
| 998 | |g 1068410221 |a Röth, Ralph |m 1068410221:Röth, Ralph |d 50000 |e 50000PR1068410221 |k 0/50000/ |p 11 | ||
| 998 | |g 121585298 |a Granzow, Martin |m 121585298:Granzow, Martin |d 910000 |d 911500 |e 910000PG121585298 |e 911500PG121585298 |k 0/910000/ |k 1/910000/911500/ |p 6 | ||
| 998 | |g 1130687643 |a Stanifer, Megan |m 1130687643:Stanifer, Megan |d 910000 |d 911700 |e 910000PS1130687643 |e 911700PS1130687643 |k 0/910000/ |k 1/910000/911700/ |p 4 | ||
| 998 | |g 1161443738 |a Martinez, Cristina |m 1161443738:Martinez, Cristina |d 910000 |d 911500 |e 910000PM1161443738 |e 911500PM1161443738 |k 0/910000/ |k 1/910000/911500/ |p 1 |x j | ||
| 999 | |a KXP-PPN1576839966 |e 301373213X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Published online first 12 January 2017","Gesehen am 26.06.2018"],"recId":"1576839966","name":{"displayForm":["Cristina Martínez, Bruno K. Rodiño-Janeiro, Beatriz Lobo, Megan L. Stanifer, Bernd Klaus, Martin Granzow, Ana M. González-Castro, Eloisa Salvo-Romero, Carmen Alonso-Cotoner, Marc Pigrau, Ralph Roeth, Gudrun Rappold, Wolfgang Huber, Rosa González-Silos, Justo Lorenzo, Inés de Torres, Fernando Azpiroz, Steeve Boulant, María Vicario, Beate Niesler, Javier Santos"]},"relHost":[{"origin":[{"dateIssuedKey":"1960","publisher":"BMJ Publishing Group","publisherPlace":"London","dateIssuedDisp":"1960-"}],"id":{"zdb":["1492637-4"],"issn":["1468-3288"],"eki":["302725628"]},"type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"part":{"volume":"66","pages":"1537-1538","text":"66(2017), 9, Seite 1537-1538","year":"2017","extent":"2","issue":"9"},"titleAlt":[{"title":"Gut online"},{"title":"eGut"}],"title":[{"subtitle":"an international journal of gastroenterology and hepatology","title":"Gut","title_sort":"Gut"}],"physDesc":[{"extent":"Online-Ressource"}],"disp":"miR-16 and miR-125b are involved in barrier function dysregulation through the modulation of claudin-2 and cingulin expression in the jejunum in IBS with diarrhoeaGut","note":["Gesehen am 07.09.12"],"pubHistory":["1.1960 -"],"recId":"302725628"}],"person":[{"role":"aut","display":"Martinez, Cristina","family":"Martinez","given":"Cristina"},{"role":"aut","family":"Stanifer","display":"Stanifer, Megan","given":"Megan"},{"given":"Martin","display":"Granzow, Martin","family":"Granzow","role":"aut"},{"role":"aut","family":"Röth","display":"Röth, Ralph","given":"Ralph"},{"family":"Rappold","display":"Rappold, Gudrun","given":"Gudrun","role":"aut"},{"display":"Huber, Wolfgang","family":"Huber","given":"Wolfgang","role":"aut"},{"role":"aut","given":"Rosa","display":"González Silos, Rosa","family":"González Silos"},{"role":"aut","given":"Justo","display":"Lorenzo Bermejo, Justo","family":"Lorenzo Bermejo"},{"given":"Steeve","family":"Boulant","display":"Boulant, Steeve","role":"aut"},{"family":"Niesler","display":"Niesler, Beate","given":"Beate","role":"aut"}],"physDesc":[{"extent":"2 S."}],"title":[{"title":"miR-16 and miR-125b are involved in barrier function dysregulation through the modulation of claudin-2 and cingulin expression in the jejunum in IBS with diarrhoea","title_sort":"miR-16 and miR-125b are involved in barrier function dysregulation through the modulation of claudin-2 and cingulin expression in the jejunum in IBS with diarrhoea"}],"origin":[{"dateIssuedDisp":"2017","dateIssuedKey":"2017"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"id":{"doi":["10.1136/gutjnl-2016-311477"],"eki":["1576839966"]},"language":["eng"]} | ||
| SRT | |a MARTINEZCRMIR16ANDMI2017 | ||