PEG-tethered guanosine acetal conjugates for the enzymatic synthesis of modified RNA
A short and efficient synthetic route to guanosine monophosphate conjugates connected to a protected aldehyde group via a flexible polydisperse polyethylene glycol linker is described. These conjugates are enzymatically incorporated into RNA transcripts with 52% yield. The resulting aldehyde-modifie...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2012
|
| In: |
Biochemical and biophysical research communications
Year: 2012, Jahrgang: 417, Heft: 4, Pages: 1224-1226 |
| ISSN: | 1090-2104 |
| DOI: | 10.1016/j.bbrc.2011.12.114 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1016/j.bbrc.2011.12.114 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0006291X11023230 
|
| Verfasserangaben: | Florentine Wahl, Andres Jäschke |
| Zusammenfassung: | A short and efficient synthetic route to guanosine monophosphate conjugates connected to a protected aldehyde group via a flexible polydisperse polyethylene glycol linker is described. These conjugates are enzymatically incorporated into RNA transcripts with 52% yield. The resulting aldehyde-modified RNA gave full conversion in the reaction with biotin hydrazide, demonstrating the utility of the method. |
|---|---|
| Beschreibung: | Available online 2 January 2012 Gesehen am 28.06.2018 |
| Beschreibung: | Online Resource |
| ISSN: | 1090-2104 |
| DOI: | 10.1016/j.bbrc.2011.12.114 |