PEG-tethered guanosine acetal conjugates for the enzymatic synthesis of modified RNA
A short and efficient synthetic route to guanosine monophosphate conjugates connected to a protected aldehyde group via a flexible polydisperse polyethylene glycol linker is described. These conjugates are enzymatically incorporated into RNA transcripts with 52% yield. The resulting aldehyde-modifie...
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| Main Authors: | , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2012
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| In: |
Biochemical and biophysical research communications
Year: 2012, Volume: 417, Issue: 4, Pages: 1224-1226 |
| ISSN: | 1090-2104 |
| DOI: | 10.1016/j.bbrc.2011.12.114 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1016/j.bbrc.2011.12.114 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0006291X11023230 
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| Author Notes: | Florentine Wahl, Andres Jäschke |
MARC
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| 520 | |a A short and efficient synthetic route to guanosine monophosphate conjugates connected to a protected aldehyde group via a flexible polydisperse polyethylene glycol linker is described. These conjugates are enzymatically incorporated into RNA transcripts with 52% yield. The resulting aldehyde-modified RNA gave full conversion in the reaction with biotin hydrazide, demonstrating the utility of the method. | ||
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