A comprehensive high-resolution targeted workflow for the deep profiling of sphingolipids
Sphingolipids make up a highly diverse group of biomolecules that not only are membrane components but also are involved in various cellular functions such as signaling and protein sorting. To obtain a quantitative view of the sphingolipidome, sensitive, accurate, and comprehensive methods are neede...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
October 17, 2017
|
| In: |
Analytical chemistry
Year: 2017, Jahrgang: 89, Heft: 22, Pages: 12480-12487 |
| ISSN: | 1520-6882 |
| DOI: | 10.1021/acs.analchem.7b03576 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1021/acs.analchem.7b03576 Verlag, Volltext: https://doi.org/10.1021/acs.analchem.7b03576 
|
| Verfasserangaben: | Bing Peng, Susan T. Weintraub, Cristina Coman, Srigayatri Ponnaiyan, Rakesh Sharma, Björn Tews, Dominic Winter, and Robert Ahrends |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1577521293 | ||
| 003 | DE-627 | ||
| 005 | 20230426230634.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180711s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1021/acs.analchem.7b03576 |2 doi | |
| 035 | |a (DE-627)1577521293 | ||
| 035 | |a (DE-576)507521293 | ||
| 035 | |a (DE-599)BSZ507521293 | ||
| 035 | |a (OCoLC)1341013451 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 30 |2 sdnb | ||
| 100 | 1 | |a Peng, Bing |e VerfasserIn |0 (DE-588)1162594047 |0 (DE-627)1026715164 |0 (DE-576)507520793 |4 aut | |
| 245 | 1 | 2 | |a A comprehensive high-resolution targeted workflow for the deep profiling of sphingolipids |c Bing Peng, Susan T. Weintraub, Cristina Coman, Srigayatri Ponnaiyan, Rakesh Sharma, Björn Tews, Dominic Winter, and Robert Ahrends |
| 264 | 1 | |c October 17, 2017 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 11.07.2018 | ||
| 520 | |a Sphingolipids make up a highly diverse group of biomolecules that not only are membrane components but also are involved in various cellular functions such as signaling and protein sorting. To obtain a quantitative view of the sphingolipidome, sensitive, accurate, and comprehensive methods are needed. Here, we present a targeted reversed-phase liquid chromatography-high-resolution mass spectrometry-based workflow that significantly increases the accuracy of measured sphingolipids by resolving nearly isobaric and isobaric species; this is accomplished by a use of (i) an optimized extraction procedure, (ii) a segmented gradient, and (iii) parallel reaction monitoring of a sphingolipid specific fragmentation pattern. The workflow was benchmarked against an accepted sphingolipid model system, the RAW 264.7 cell line, and 61 sphingolipids were quantified over a dynamic range of 7 orders of magnitude, with detection limits in the low femtomole per milligram of protein level, making this workflow an extremely versatile tool for high-throughput sphingolipidomics. | ||
| 700 | 1 | |a Sharma, Rakesh |e VerfasserIn |0 (DE-588)1162596473 |0 (DE-627)1026717639 |0 (DE-576)507522184 |4 aut | |
| 700 | 1 | |a Tews, Björn |d 1976- |e VerfasserIn |0 (DE-588)105866655X |0 (DE-627)797388052 |0 (DE-576)34674234X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Analytical chemistry |d Columbus, Ohio : American Chemical Society, 1947 |g 89(2017), 22, Seite 12480-12487 |h Online-Ressource |w (DE-627)300896530 |w (DE-600)1483443-1 |w (DE-576)088704564 |x 1520-6882 |7 nnas |a A comprehensive high-resolution targeted workflow for the deep profiling of sphingolipids |
| 773 | 1 | 8 | |g volume:89 |g year:2017 |g number:22 |g pages:12480-12487 |g extent:8 |a A comprehensive high-resolution targeted workflow for the deep profiling of sphingolipids |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1021/acs.analchem.7b03576 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://doi.org/10.1021/acs.analchem.7b03576 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180711 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 105866655X |a Tews, Björn |m 105866655X:Tews, Björn |d 50000 |e 50000PT105866655X |k 0/50000/ |p 6 | ||
| 998 | |g 1162596473 |a Sharma, Rakesh |m 1162596473:Sharma, Rakesh |d 700000 |d 718000 |e 700000PS1162596473 |e 718000PS1162596473 |k 0/700000/ |k 1/700000/718000/ |p 5 | ||
| 999 | |a KXP-PPN1577521293 |e 3017337820 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedKey":"2017","dateIssuedDisp":"October 17, 2017"}],"id":{"eki":["1577521293"],"doi":["10.1021/acs.analchem.7b03576"]},"name":{"displayForm":["Bing Peng, Susan T. Weintraub, Cristina Coman, Srigayatri Ponnaiyan, Rakesh Sharma, Björn Tews, Dominic Winter, and Robert Ahrends"]},"physDesc":[{"extent":"8 S."}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"name":{"displayForm":["American Chemical Society"]},"origin":[{"publisherPlace":"Columbus, Ohio","publisher":"American Chemical Society","dateIssuedKey":"1947","dateIssuedDisp":"1947-"}],"id":{"eki":["300896530"],"zdb":["1483443-1"],"issn":["1520-6882"]},"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"A comprehensive high-resolution targeted workflow for the deep profiling of sphingolipidsAnalytical chemistry","note":["Gesehen am 27.08.2020","Ungezählte Beil.: News & features"],"recId":"300896530","language":["eng"],"pubHistory":["19.1947 -"],"part":{"issue":"22","pages":"12480-12487","year":"2017","extent":"8","text":"89(2017), 22, Seite 12480-12487","volume":"89"},"title":[{"title_sort":"Analytical chemistry","title":"Analytical chemistry","subtitle":"the authoritative voice of the analytical community"}]}],"title":[{"title_sort":"comprehensive high-resolution targeted workflow for the deep profiling of sphingolipids","title":"A comprehensive high-resolution targeted workflow for the deep profiling of sphingolipids"}],"person":[{"display":"Peng, Bing","roleDisplay":"VerfasserIn","role":"aut","family":"Peng","given":"Bing"},{"given":"Rakesh","family":"Sharma","role":"aut","display":"Sharma, Rakesh","roleDisplay":"VerfasserIn"},{"given":"Björn","family":"Tews","role":"aut","roleDisplay":"VerfasserIn","display":"Tews, Björn"}],"note":["Gesehen am 11.07.2018"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"recId":"1577521293"} | ||
| SRT | |a PENGBINGSHCOMPREHENS1720 | ||