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STIL is required for centriole duplication in human cells

Centrioles are key structural elements of centrosomes and primary cilia. In mammals, only a few proteins including PLK4, CPAP (CENPJ), SAS6, CEP192, CEP152 and CEP135 have thus far been identified to be required for centriole duplication. STIL (SCL/TAL1 interrupting locus, also known as SIL) is a ce...

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Main Authors: Vulprecht, Julia (Author) , Tibelius, Alexandra (Author) , Konotop, Gleb (Author) , Liu, Fengying (Author) , Raab, Marc-Steffen (Author) , Krämer, Alwin (Author)
Format: Article (Journal)
Language:English
Published: April 6, 2012
In: Journal of cell science
Year: 2012, Volume: 125, Issue: 5, Pages: 1353-1362
ISSN:1477-9137
DOI:10.1242/jcs.104109
Online Access:Verlag, Volltext: http://dx.doi.org/10.1242/jcs.104109
Verlag, Volltext: http://jcs.biologists.org/content/125/5/1353
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Author Notes:Julia Vulprecht, Ahuvit David, Alexandra Tibelius, Asher Castiel, Gleb Konotop, Fengying Liu, Felix Bestvater, Marc S. Raab, Hanswalter Zentgraf, Shai Izraeli and Alwin Krämer
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Summary:Centrioles are key structural elements of centrosomes and primary cilia. In mammals, only a few proteins including PLK4, CPAP (CENPJ), SAS6, CEP192, CEP152 and CEP135 have thus far been identified to be required for centriole duplication. STIL (SCL/TAL1 interrupting locus, also known as SIL) is a centrosomal protein that is essential for mouse and zebrafish embryonic development and mutated in primary microcephaly. Here, we show that STIL localizes to the pericentriolar material surrounding parental centrioles. Its overexpression results in excess centriole formation. siRNA-mediated depletion of STIL leads to loss of centrioles and abrogates PLK4-induced centriole overduplication. Additionally, we show that STIL is necessary for SAS6 recruitment to centrioles, suggesting that it is essential for daughter centriole formation, interacts with the centromere protein CPAP and rapidly shuttles between the cytoplasm and centrioles. Consistent with the requirement of centrioles for cilia formation, Stil-/- mouse embryonic fibroblasts lack primary cilia - a phenotype that can be reverted by restoration of STIL expression. These findings demonstrate that STIL is an essential component of the centriole replication machinery in mammalian cells.
Item Description:Published online April 6, 2012
Gesehen am 16.07.2018
Physical Description:Online Resource
ISSN:1477-9137
DOI:10.1242/jcs.104109

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