Rapid detection of ampicillin resistance in Escherichia coli by quantitative mass spectrometry
Early targeted antimicrobial therapy helps saving costs and prevents the spread of antimicrobial resistances including in Escherichia coli, the most frequent gram-negative bacterium causing sepsis. Therefore, rapid susceptibility testing represents the major prerequisite for knowledge-based successf...
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| Main Authors: | , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
May 2012
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| In: |
Journal of clinical microbiology
Year: 2012, Volume: 50, Issue: 5, Pages: 1727-1729 |
| ISSN: | 1098-660X |
| DOI: | 10.1128/JCM.00047-12 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1128/JCM.00047-12 Verlag, Volltext: http://jcm.asm.org/content/early/2012/02/02/JCM.00047-12 
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| Author Notes: | A. Grundt, P. Findeisen, T. Miethke, E. Jäger, P. Ahmad-Nejad and M. Neumaier |
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| 520 | |a Early targeted antimicrobial therapy helps saving costs and prevents the spread of antimicrobial resistances including in Escherichia coli, the most frequent gram-negative bacterium causing sepsis. Therefore, rapid susceptibility testing represents the major prerequisite for knowledge-based successful antimicrobial treatment. To accelerate testing for antibiotic susceptibility, we have developed a new mass spectrometry-based assay for antibiotic susceptibility testing (MAAST). For proof of principle we present an ampicillin susceptibility test for E.coli with a TAT of 90 minutes upon growth detection. | ||
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