In vivo EROD assays with the zebrafish (Danio rerio) as rapid screening tools for the detection of dioxin-like activity
The present study compares two alternative in vivo approaches for the measurement of ethoxyresorufin-O-deethylase (EROD) activity in zebrafish (Danio rerio) following exposure to acetonic model sediment extracts: (1) the live-imaging EROD assay for the direct detection of EROD induction in individua...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
6 March 2017
|
| In: |
The science of the total environment
Year: 2017, Jahrgang: 590/591, Pages: 269-280 |
| ISSN: | 1879-1026 |
| DOI: | 10.1016/j.scitotenv.2017.02.236 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1016/j.scitotenv.2017.02.236 Verlag, Volltext: https://www.sciencedirect.com/science/article/pii/S0048969717305016?via%3Dihub 
|
| Verfasserangaben: | Britta Kais, Sabrina Schiwy, Henner Hollert, Steffen H. Keiter, Thomas Braunbeck |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1577638905 | ||
| 003 | DE-627 | ||
| 005 | 20220814192931.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180716s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.scitotenv.2017.02.236 |2 doi | |
| 035 | |a (DE-627)1577638905 | ||
| 035 | |a (DE-576)507638905 | ||
| 035 | |a (DE-599)BSZ507638905 | ||
| 035 | |a (OCoLC)1341013732 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Kais, Britta |e VerfasserIn |0 (DE-588)1069019496 |0 (DE-627)821169122 |0 (DE-576)428367216 |4 aut | |
| 245 | 1 | 0 | |a In vivo EROD assays with the zebrafish (Danio rerio) as rapid screening tools for the detection of dioxin-like activity |c Britta Kais, Sabrina Schiwy, Henner Hollert, Steffen H. Keiter, Thomas Braunbeck |
| 264 | 1 | |c 6 March 2017 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 16.07.2018 | ||
| 520 | |a The present study compares two alternative in vivo approaches for the measurement of ethoxyresorufin-O-deethylase (EROD) activity in zebrafish (Danio rerio) following exposure to acetonic model sediment extracts: (1) the live-imaging EROD assay for the direct detection of EROD induction in individual livers via epifluorescence, and (2) the fish embryo EROD assay in subcellular fractions derived from entire zebrafish embryos after in vivo exposure. For toxicity assessment, each sediment extract was tested with the standard fish embryo test (FET). Upon completion of a functioning liver after 72h, the embryos gave a distinct fluorescent signal in the liver, and a corresponding EROD activity could be detected in the fish embryo EROD assay. The exposure time in the live-imaging EROD assay was reduced to 3h, which resulted in a stronger, less variable and more sensitive EROD response. Overall, the live-imaging and the fish embryo EROD assays showed the same tendencies and gave comparable results, e.g. a concentration-dependent increase in EROD activity at concentrations one order of magnitude below concentrations producing macroscopically visible abnormalities. At higher concentrations, however, a decrease of EROD activity was observed in either test. Both tests ranked the three model sediment extracts in the same order. Results indicate that both test systems complement each other and together provide a rapid and reliable in vivo tool to investigate the presence of dioxin-like substances in environmental samples. | ||
| 650 | 4 | |a CYP 1A | |
| 650 | 4 | |a Embryo | |
| 650 | 4 | |a EROD induction | |
| 650 | 4 | |a In vivo | |
| 650 | 4 | |a Live-imaging | |
| 650 | 4 | |a Sediment | |
| 650 | 4 | |a Zebrafish | |
| 700 | 1 | |a Braunbeck, Thomas |e VerfasserIn |0 (DE-588)106835819X |0 (DE-627)82017839X |0 (DE-576)165906731 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The science of the total environment |d Amsterdam [u.a.] : Elsevier Science, 1972 |g 590/591(2017), Seite 269-280 |h Online-Ressource |w (DE-627)306591456 |w (DE-600)1498726-0 |w (DE-576)081953178 |x 1879-1026 |7 nnas |a In vivo EROD assays with the zebrafish (Danio rerio) as rapid screening tools for the detection of dioxin-like activity |
| 773 | 1 | 8 | |g volume:590/591 |g year:2017 |g pages:269-280 |g extent:12 |a In vivo EROD assays with the zebrafish (Danio rerio) as rapid screening tools for the detection of dioxin-like activity |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |a Kais, Britta |t In vivo EROD assays with the zebrafish (Danio rerio) as rapid screening tools for the detection of dioxin-like activity |d 2017 |w (DE-627)157764199X |w (DE-576)50764199X |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.scitotenv.2017.02.236 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0048969717305016?via%3Dihub |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180716 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 106835819X |a Braunbeck, Thomas |m 106835819X:Braunbeck, Thomas |d 700000 |d 721000 |e 700000PB106835819X |e 721000PB106835819X |k 0/700000/ |k 1/700000/721000/ |p 5 |y j | ||
| 998 | |g 1069019496 |a Kais, Britta |m 1069019496:Kais, Britta |d 700000 |d 721000 |e 700000PK1069019496 |e 721000PK1069019496 |k 0/700000/ |k 1/700000/721000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1577638905 |e 3017617629 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 16.07.2018"],"origin":[{"dateIssuedKey":"2017","dateIssuedDisp":"6 March 2017"}],"person":[{"role":"aut","display":"Kais, Britta","family":"Kais","given":"Britta"},{"given":"Thomas","family":"Braunbeck","display":"Braunbeck, Thomas","role":"aut"}],"title":[{"title":"In vivo EROD assays with the zebrafish (Danio rerio) as rapid screening tools for the detection of dioxin-like activity","title_sort":"In vivo EROD assays with the zebrafish (Danio rerio) as rapid screening tools for the detection of dioxin-like activity"}],"relHost":[{"title":[{"subtitle":"an international journal for scientific research into the environment and its relationship with man","title":"The science of the total environment","title_sort":"science of the total environment"}],"part":{"text":"590/591(2017), Seite 269-280","pages":"269-280","extent":"12","year":"2017","volume":"590/591"},"disp":"In vivo EROD assays with the zebrafish (Danio rerio) as rapid screening tools for the detection of dioxin-like activityThe science of the total environment","type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"note":["Gesehen am 11.04.2023"],"origin":[{"publisher":"Elsevier Science","dateIssuedDisp":"1972-","dateIssuedKey":"1972","publisherPlace":"Amsterdam [u.a.]"}],"physDesc":[{"extent":"Online-Ressource"}],"recId":"306591456","pubHistory":["1.1972/73 - 409.2010; Vol. 410/411.2011 -"],"id":{"issn":["1879-1026"],"eki":["306591456"],"zdb":["1498726-0"]}}],"physDesc":[{"extent":"12 S."}],"language":["eng"],"name":{"displayForm":["Britta Kais, Sabrina Schiwy, Henner Hollert, Steffen H. Keiter, Thomas Braunbeck"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"recId":"1577638905","id":{"doi":["10.1016/j.scitotenv.2017.02.236"],"eki":["1577638905"]}} | ||
| SRT | |a KAISBRITTAINVIVOEROD6201 | ||