Superresolution imaging of transcription units on newt lampbrush chromosomes
We have examined transcription loops on lampbrush chromosomes of the newt Notophthalmus by superresolution microscopy. Because of the favorable, essentially two-dimensional morphology of these loops, an average optical resolution in the x-y plane of about 50 nm was achieved. We analyzed the distribu...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
15 August 2012
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| In: |
Chromosome research
Year: 2012, Volume: 20, Issue: 8, Pages: 1009-1015 |
| ISSN: | 1573-6849 |
| DOI: | 10.1007/s10577-012-9306-z |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1007/s10577-012-9306-z Verlag, Volltext: https://link.springer.com/article/10.1007/s10577-012-9306-z 
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| Author Notes: | Rainer Kaufmann, Christoph Cremer, Joseph G. Gall |
| Summary: | We have examined transcription loops on lampbrush chromosomes of the newt Notophthalmus by superresolution microscopy. Because of the favorable, essentially two-dimensional morphology of these loops, an average optical resolution in the x-y plane of about 50 nm was achieved. We analyzed the distribution of the multifunctional RNA-binding protein CELF1 on specific loops. CELF1 distribution is consistent with a model in which individual transcripts are tightly folded and hence closely packed against the loop axis. |
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| Item Description: | Gesehen am 19.07.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1573-6849 |
| DOI: | 10.1007/s10577-012-9306-z |