Regulation of integrin adhesions by varying the density of substrate-bound epidermal growth factor
Substrates coated with specific bioactive ligands are important for tissue engineering, enabling the local presentation of extracellular stimulants at controlled positions and densities. In this study, we examined the cross-talk between integrin and epidermal growth factor (EGF) receptors following...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
3 March 2012
|
| In: |
Biointerphases
Year: 2012, Volume: 7, Issue: 1 |
| ISSN: | 1559-4106 |
| DOI: | 10.1007/s13758-012-0023-0 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1007/s13758-012-0023-0 Verlag, kostenfrei, Volltext: https://pubs.aip.org/avs/bip/article/7/1/23/134048/Regulation-of-Integrin-Adhesions-by-Varying-the 
|
| Author Notes: | Tamar Shahal, Benjamin Geiger, Iain E. Dunlop, Joachim P. Spatz |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1577828607 | ||
| 003 | DE-627 | ||
| 005 | 20241009115102.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180723s2012 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s13758-012-0023-0 |2 doi | |
| 035 | |a (DE-627)1577828607 | ||
| 035 | |a (DE-576)507828607 | ||
| 035 | |a (DE-599)BSZ507828607 | ||
| 035 | |a (OCoLC)1341014211 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 30 |2 sdnb | ||
| 100 | 1 | |a Shahal, Tamar |e VerfasserIn |0 (DE-588)1163211109 |0 (DE-627)1027434452 |0 (DE-576)507828186 |4 aut | |
| 245 | 1 | 0 | |a Regulation of integrin adhesions by varying the density of substrate-bound epidermal growth factor |c Tamar Shahal, Benjamin Geiger, Iain E. Dunlop, Joachim P. Spatz |
| 264 | 1 | |c 3 March 2012 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 23.07.2018 | ||
| 520 | |a Substrates coated with specific bioactive ligands are important for tissue engineering, enabling the local presentation of extracellular stimulants at controlled positions and densities. In this study, we examined the cross-talk between integrin and epidermal growth factor (EGF) receptors following their interaction with surface-immobilized Arg-Gly-Asp (RGD) and EGF ligands, respectively. Surfaces of glass coverslips, modified with biotinylated silane-polyethylene glycol, were functionalized by either biotinylated RGD or EGF (or both) via the biotin-NeutrAvidin interaction. Fluorescent labeling of the adhering A431 epidermoid carcinoma cells for zyxin or actin indicated that EGF had a dual effect on focal adhesions (FA) and stress fibers: at low concentrations (0.1; 1 ng/ml), it stimulated their growth; whereas at higher concentrations, on surfaces with low to intermediate RGD densities, it induced their disassembly, leading to cell detachment. The EGF- dependent dissociation of FAs was, however, attenuated on higher RGD density surfaces. Simultaneous stimulation by both immobilized RGD and EGF suggest a strong synergy between integrin and EGFR signaling, in FA induction and cell spreading. A critical threshold level of EGF was required to induce significant variation in cell adhesion; beyond this critical density, the immobilized molecule had a considerably stronger effect on cell adhesion than did soluble EGF. The mechanisms underlying this synergy between the adhesion ligand and EGF are discussed. | ||
| 700 | 1 | |a Spatz, Joachim P. |d 1969- |e VerfasserIn |0 (DE-588)122582675 |0 (DE-627)08203124X |0 (DE-576)173014771 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biointerphases |d Melville, NY : AIP, 2006 |g 7(2012,1) Artikel-Nummer 23, 11 Seiten |h Online-Ressource |w (DE-627)511913915 |w (DE-600)2234510-3 |w (DE-576)281308888 |x 1559-4106 |7 nnas |a Regulation of integrin adhesions by varying the density of substrate-bound epidermal growth factor |
| 773 | 1 | 8 | |g volume:7 |g year:2012 |g number:1 |g extent:11 |a Regulation of integrin adhesions by varying the density of substrate-bound epidermal growth factor |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s13758-012-0023-0 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://pubs.aip.org/avs/bip/article/7/1/23/134048/Regulation-of-Integrin-Adhesions-by-Varying-the |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180723 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 122582675 |a Spatz, Joachim P. |m 122582675:Spatz, Joachim P. |d 120000 |d 120300 |e 120000PS122582675 |e 120300PS122582675 |k 0/120000/ |k 1/120000/120300/ |p 4 |y j | ||
| 999 | |a KXP-PPN1577828607 |e 3019336864 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title_sort":"Regulation of integrin adhesions by varying the density of substrate-bound epidermal growth factor","title":"Regulation of integrin adhesions by varying the density of substrate-bound epidermal growth factor"}],"person":[{"family":"Shahal","given":"Tamar","roleDisplay":"VerfasserIn","display":"Shahal, Tamar","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Spatz, Joachim P.","role":"aut","family":"Spatz","given":"Joachim P."}],"note":["Gesehen am 23.07.2018"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"recId":"1577828607","origin":[{"dateIssuedDisp":"3 March 2012","dateIssuedKey":"2012"}],"id":{"doi":["10.1007/s13758-012-0023-0"],"eki":["1577828607"]},"name":{"displayForm":["Tamar Shahal, Benjamin Geiger, Iain E. Dunlop, Joachim P. Spatz"]},"physDesc":[{"extent":"11 S."}],"relHost":[{"corporate":[{"role":"isb","display":"American Vacuum Society","roleDisplay":"Herausgebendes Organ"}],"language":["eng"],"recId":"511913915","type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Regulation of integrin adhesions by varying the density of substrate-bound epidermal growth factorBiointerphases","note":["Titelzusatz früher: an open access journal for the Biomaterials Interface community","Gesehen am 25.04.2023"],"part":{"volume":"7","text":"7(2012,1) Artikel-Nummer 23, 11 Seiten","extent":"11","year":"2012","issue":"1"},"pubHistory":["1.2006 -"],"title":[{"title_sort":"Biointerphases","title":"Biointerphases"}],"physDesc":[{"extent":"Online-Ressource"}],"name":{"displayForm":["American Vacuum Society"]},"id":{"zdb":["2234510-3"],"eki":["511913915"],"issn":["1559-4106"]},"origin":[{"dateIssuedDisp":"2006-","dateIssuedKey":"2006","publisher":"AIP ; AVS ; Springer","publisherPlace":"Melville, NY ; New York, NY ; Heidelberg ; Berlin"}]}]} | ||
| SRT | |a SHAHALTAMAREGULATION3201 | ||