The receptor for advanced glycation end products RAGE is involved in corneal healing
Impaired corneal healing is still a major cause of blindness. As RAGE (receptor for advanced glycation endproducts) is involved in inflammation and wound healing in other tissues, we here investigated its relevance for corneal wound healing. Corneal re-epithelialization after alkaline injury was ana...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
3 February 2017
|
| In: |
Annals of anatomy
Year: 2017, Jahrgang: 211, Pages: 13-20 |
| ISSN: | 1618-0402 |
| DOI: | 10.1016/j.aanat.2017.01.003 |
| Online-Zugang: | Resolving-System, Volltext: http://dx.doi.org/10.1016/j.aanat.2017.01.003 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0940960217300031 
|
| Verfasserangaben: | Norbert Nass, Stefanie Trau, Friedrich Paulsen, Delia Kaiser, Thomas Kalinski, Saadettin Sel |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1577916859 | ||
| 003 | DE-627 | ||
| 005 | 20220814200920.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180725s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.aanat.2017.01.003 |2 doi | |
| 035 | |a (DE-627)1577916859 | ||
| 035 | |a (DE-576)507916859 | ||
| 035 | |a (DE-599)BSZ507916859 | ||
| 035 | |a (OCoLC)1341014450 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Naß, Norbert |e VerfasserIn |0 (DE-588)1163325562 |0 (DE-627)1027623921 |0 (DE-576)507917235 |4 aut | |
| 245 | 1 | 4 | |a The receptor for advanced glycation end products RAGE is involved in corneal healing |c Norbert Nass, Stefanie Trau, Friedrich Paulsen, Delia Kaiser, Thomas Kalinski, Saadettin Sel |
| 264 | 1 | |c 3 February 2017 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Available online 3 February 2017 | ||
| 500 | |a Gesehen am 25.07.2018 | ||
| 520 | |a Impaired corneal healing is still a major cause of blindness. As RAGE (receptor for advanced glycation endproducts) is involved in inflammation and wound healing in other tissues, we here investigated its relevance for corneal wound healing. Corneal re-epithelialization after alkaline injury was analysed in an ex-vivo approach with cultured, enucleated eyes from mice either of the C57Bl/6 NChR genotype (RAGE+/+) and mice of the same strain lacking the RAGE gene (RAGE−/−). The wound area was determined time dependently by fluorescence imaging using fluorescein staining. The eyes of RAGE−/− mice showed a significantly slower re-epithelialization than eyes of the RAGE+/− and the RAGE+/+ genotype. In immunohistochemistry, RAGE expression was increased in wounded corneas whereas the abundance of the RAGE ligand HMGB1 was unaffected, but an increase in S100b-like proteins was revealed upon injury. However, neither the addition of the RAGE agonist HMGB1 or an HMGB1 antagonising antibody nor bovine S100b protein to the culture medium of the wounded eyes had an effect on corneal wound closure in ex-vivo. Further gene expression analysis by RT-PCR demonstrated an increase in RAGE expression on the mRNA level, no significant regulation of HMGB1 and a differential regulation of the S100 gene family after alkaline burn of the cornea. In conclusion, RAGE is clearly involved in corneal re-epithelialization most probably mediated by signalling via S100 proteins. | ||
| 650 | 4 | |a Advanced glycation end products | |
| 650 | 4 | |a Cornea | |
| 650 | 4 | |a HMGB-1 | |
| 650 | 4 | |a RAGE | |
| 650 | 4 | |a S100 proteins | |
| 650 | 4 | |a wound healing | |
| 700 | 1 | |a Kaiser, Delia |e VerfasserIn |0 (DE-588)1160217289 |0 (DE-627)1023352249 |0 (DE-576)505567679 |4 aut | |
| 700 | 1 | |a Sel, Saadettin |d 1968- |e VerfasserIn |0 (DE-588)122466926 |0 (DE-627)081961472 |0 (DE-576)293284474 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Annals of anatomy |d Jena : Elsevier, 1992 |g 211(2017), Seite 13-20 |h Online-Ressource |w (DE-627)483300594 |w (DE-600)2182566-X |w (DE-576)252074742 |x 1618-0402 |7 nnas |a The receptor for advanced glycation end products RAGE is involved in corneal healing |
| 773 | 1 | 8 | |g volume:211 |g year:2017 |g pages:13-20 |g extent:8 |a The receptor for advanced glycation end products RAGE is involved in corneal healing |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.aanat.2017.01.003 |x Resolving-System |x Verlag |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0940960217300031 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180725 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 122466926 |a Sel, Saadettin |m 122466926:Sel, Saadettin |d 910000 |d 910900 |e 910000PS122466926 |e 910900PS122466926 |k 0/910000/ |k 1/910000/910900/ |p 6 |y j | ||
| 998 | |g 1160217289 |a Kaiser, Delia |m 1160217289:Kaiser, Delia |d 910000 |d 910900 |e 910000PK1160217289 |e 910900PK1160217289 |k 0/910000/ |k 1/910000/910900/ |p 4 | ||
| 999 | |a KXP-PPN1577916859 |e 301947566X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedDisp":"3 February 2017","dateIssuedKey":"2017"}],"id":{"eki":["1577916859"],"doi":["10.1016/j.aanat.2017.01.003"]},"name":{"displayForm":["Norbert Nass, Stefanie Trau, Friedrich Paulsen, Delia Kaiser, Thomas Kalinski, Saadettin Sel"]},"physDesc":[{"extent":"8 S."}],"relHost":[{"title":[{"title_sort":"Annals of anatomy","subtitle":"official organ of the Anatomische Gesellschaft = Anatomischer Anzeiger","title":"Annals of anatomy"}],"part":{"volume":"211","text":"211(2017), Seite 13-20","extent":"8","year":"2017","pages":"13-20"},"pubHistory":["174.1992 -"],"titleTranslated":[{"translated":"Anatomischer Anzeiger"}],"recId":"483300594","language":["eng"],"type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 09.11.2020"],"disp":"The receptor for advanced glycation end products RAGE is involved in corneal healingAnnals of anatomy","id":{"issn":["1618-0402"],"zdb":["2182566-X"],"eki":["483300594"]},"origin":[{"publisherPlace":"Jena","dateIssuedDisp":"1992-","publisher":"Elsevier","dateIssuedKey":"1992"}],"physDesc":[{"extent":"Online-Ressource"}]}],"title":[{"title":"The receptor for advanced glycation end products RAGE is involved in corneal healing","title_sort":"receptor for advanced glycation end products RAGE is involved in corneal healing"}],"person":[{"family":"Naß","given":"Norbert","display":"Naß, Norbert","roleDisplay":"VerfasserIn","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Kaiser, Delia","role":"aut","family":"Kaiser","given":"Delia"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Sel, Saadettin","given":"Saadettin","family":"Sel"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Available online 3 February 2017","Gesehen am 25.07.2018"],"recId":"1577916859","language":["eng"]} | ||
| SRT | |a NASSNORBERRECEPTORFO3201 | ||