Evaluation of Alere i RSV for rapid detection of respiratory syncytial virus in children hospitalized with acute respiratory tract infection
Alere™ i RSV is a novel rapid test which applies a nicking enzyme amplification reaction to detect respiratory syncytial virus in point-of-care settings. In this study, we evaluated the Alere™ i RSV assay by using frozen nasopharyngeal swab samples that were collected in viral transport medium from...
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| Main Authors: | , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
11 January 2017
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| In: |
Journal of clinical microbiology
Year: 2017, Volume: 55, Issue: 4, Pages: 1032-1036 |
| ISSN: | 1098-660X |
| DOI: | 10.1128/JCM.02433-16 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1128/JCM.02433-16 Verlag, Volltext: http://jcm.asm.org/content/early/2017/01/05/JCM.02433-16 
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| Author Notes: | Rebecca Marie Peters, Sarah Valerie Schnee, Julia Tabatabai, Paul Schnitzler, Johannes Pfeil |
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| 520 | |a Alere™ i RSV is a novel rapid test which applies a nicking enzyme amplification reaction to detect respiratory syncytial virus in point-of-care settings. In this study, we evaluated the Alere™ i RSV assay by using frozen nasopharyngeal swab samples that were collected in viral transport medium from children hospitalized with acute respiratory tract infection during winter season 2015/2016. Alere™ i RSV assay test results were compared to Altona® RealStar RSV real-time reverse transcription PCR (RT-PCR). We found that the overall sensitivity and specificity of the Alere™ i RSV test assay was 100% (CI95 93% - 100%) and 97% (CI95 89% - 100%), respectively. Positive samples were identified within 5-7 minutes from sample collection. Overall, the Alere™ i RSV test assay performed well in comparison to the RT-PCR assay and has the potential to facilitate the detection of RSV in point-of-care settings. | ||
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