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Evaluation of Alere i RSV for rapid detection of respiratory syncytial virus in children hospitalized with acute respiratory tract infection

Alere™ i RSV is a novel rapid test which applies a nicking enzyme amplification reaction to detect respiratory syncytial virus in point-of-care settings. In this study, we evaluated the Alere™ i RSV assay by using frozen nasopharyngeal swab samples that were collected in viral transport medium from...

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Bibliographische Detailangaben
Hauptverfasser: Peters, Rebecca (VerfasserIn) , Schnee, Sarah Valerie (VerfasserIn) , Tabatabai, Julia (VerfasserIn) , Schnitzler, Paul (VerfasserIn) , Pfeil, Johannes (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 11 January 2017
In: Journal of clinical microbiology
Year: 2017, Jahrgang: 55, Heft: 4, Pages: 1032-1036
ISSN:1098-660X
DOI:10.1128/JCM.02433-16
Online-Zugang:Verlag, Volltext: http://dx.doi.org/10.1128/JCM.02433-16
Verlag, Volltext: http://jcm.asm.org/content/early/2017/01/05/JCM.02433-16
Volltext
Verfasserangaben:Rebecca Marie Peters, Sarah Valerie Schnee, Julia Tabatabai, Paul Schnitzler, Johannes Pfeil
Beschreibung
Zusammenfassung:Alere™ i RSV is a novel rapid test which applies a nicking enzyme amplification reaction to detect respiratory syncytial virus in point-of-care settings. In this study, we evaluated the Alere™ i RSV assay by using frozen nasopharyngeal swab samples that were collected in viral transport medium from children hospitalized with acute respiratory tract infection during winter season 2015/2016. Alere™ i RSV assay test results were compared to Altona® RealStar RSV real-time reverse transcription PCR (RT-PCR). We found that the overall sensitivity and specificity of the Alere™ i RSV test assay was 100% (CI95 93% - 100%) and 97% (CI95 89% - 100%), respectively. Positive samples were identified within 5-7 minutes from sample collection. Overall, the Alere™ i RSV test assay performed well in comparison to the RT-PCR assay and has the potential to facilitate the detection of RSV in point-of-care settings.
Beschreibung:Accepted manuscript posted online11January 2017
Gesehen am 31.07.2018
Beschreibung:Online Resource
ISSN:1098-660X
DOI:10.1128/JCM.02433-16

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