Genetic suppression of atrial fibrillation using a dominant-negative ether-a-go-go-related gene mutant
Background: Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia. Gene therapy-dependent modulation of atrial electrophysiology may provide a more specific alternative to pharmacological and ablative treatment strategies. Objective: We hypothesized that genetic inactivation of at...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2012
|
| In: |
Heart rhythm
Year: 2011, Volume: 9, Issue: 2, Pages: 265-272 |
| ISSN: | 1556-3871 |
| DOI: | 10.1016/j.hrthm.2011.09.008 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1016/j.hrthm.2011.09.008 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S1547527111010459 
|
| Author Notes: | Radim Soucek, MD, Dierk Thomas, MD, Kamilla Kelemen, MD, Olympia Bikou, MD, Claudia Seyler, PhD, Frederik Voss, MD, Rüdiger Becker, MD, Michael Koenen, PhD, Hugo A. Katus, MD, Alexander Bauer, MD |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1578206448 | ||
| 003 | DE-627 | ||
| 005 | 20230426230830.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180802r20122011xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.hrthm.2011.09.008 |2 doi | |
| 035 | |a (DE-627)1578206448 | ||
| 035 | |a (DE-576)508206448 | ||
| 035 | |a (DE-599)BSZ508206448 | ||
| 035 | |a (OCoLC)1341016153 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Soucek, Radim |d 1983- |e VerfasserIn |0 (DE-588)1020322519 |0 (DE-627)691180059 |0 (DE-576)357985729 |4 aut | |
| 245 | 1 | 0 | |a Genetic suppression of atrial fibrillation using a dominant-negative ether-a-go-go-related gene mutant |c Radim Soucek, MD, Dierk Thomas, MD, Kamilla Kelemen, MD, Olympia Bikou, MD, Claudia Seyler, PhD, Frederik Voss, MD, Rüdiger Becker, MD, Michael Koenen, PhD, Hugo A. Katus, MD, Alexander Bauer, MD |
| 264 | 1 | |c 2012 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Available online 8 September 2011 | ||
| 500 | |a Gesehen am 02.08.2018 | ||
| 520 | |a Background: Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia. Gene therapy-dependent modulation of atrial electrophysiology may provide a more specific alternative to pharmacological and ablative treatment strategies. Objective: We hypothesized that genetic inactivation of atrial repolarizing ether-a-go-go-related gene (ERG) K+ currents using a dominant-negative mutant would provide rhythm control in AF. Methods: Ten domestic swine underwent pacemaker implantation and were subjected to atrial burst pacing to induce persistent AF. Animals were then randomized to receive either AdCERG-G627S to suppress ERG/IKr currents or green fluorescent protein (AdGFP) as control. Adenoviruses were applied using a novel hybrid technique combining atrial virus injection and epicardial electroporation to increase transgene expression. Results: In pigs treated with AdCERG-G627S, the onset of persistent AF was prevented (n = 2) or significantly delayed compared with AdGFP controls (12 ± 2.1 vs. 6.2 ± 1.3 days; P < .001) during 14-day follow-up. Effective refractory periods were prolonged in the AdCERG-G627S group compared with AdGFP animals (221.5 ± 4.7 ms vs. 197.0 ± 4.7 ms; P < .006). Impairment of left ventricular ejection fraction (LVEF) during AF was prevented by AdCERG-G627S application (LVEFCERG-G627S = 62.1% ± 4.0% vs. LVEFGFP = 30.3% ± 9.1%; P < .001). Conclusion: Inhibition of ERG function using atrial AdCERG-G627S gene transfer suppresses or delays the onset of persistent AF by prolongation of atrial refractoriness in a porcine model. Targeted gene therapy represents an alternative to pharmacological or ablative treatment of AF. | ||
| 534 | |c 2011 | ||
| 650 | 4 | |a Atrial fibrillation | |
| 650 | 4 | |a Gene therapy | |
| 650 | 4 | |a HERG | |
| 650 | 4 | |a Ion channel | |
| 650 | 4 | |a Rhythm control | |
| 700 | 1 | |a Thomas, Dierk |d 1974- |e VerfasserIn |0 (DE-588)123896258 |0 (DE-627)706465601 |0 (DE-576)29393164X |4 aut | |
| 700 | 1 | |a Kelemen, Kamilla |d 1976- |e VerfasserIn |0 (DE-588)129031933 |0 (DE-627)387884386 |0 (DE-576)29745742X |4 aut | |
| 700 | 1 | |a Bikou, Olympia |e VerfasserIn |0 (DE-588)1024753360 |0 (DE-627)720453569 |0 (DE-576)36944096X |4 aut | |
| 700 | 1 | |a Seyler, Claudia |d 1976- |e VerfasserIn |0 (DE-588)13306476X |0 (DE-627)533316871 |0 (DE-576)299599523 |4 aut | |
| 700 | 1 | |a Voss, Frederik |d 1968- |e VerfasserIn |0 (DE-588)122527364 |0 (DE-627)705940152 |0 (DE-576)293308845 |4 aut | |
| 700 | 1 | |a Becker, Rüdiger |e VerfasserIn |0 (DE-588)1032502541 |0 (DE-627)738501409 |0 (DE-576)380071061 |4 aut | |
| 700 | 1 | |a Koenen, Michael |e VerfasserIn |0 (DE-588)1059101904 |0 (DE-627)797963774 |0 (DE-576)415127726 |4 aut | |
| 700 | 1 | |a Katus, Hugo |d 1951- |e VerfasserIn |0 (DE-588)108916618 |0 (DE-627)577155040 |0 (DE-576)289625076 |4 aut | |
| 700 | 1 | |a Bauer, Alexander |d 1966- |e VerfasserIn |0 (DE-588)115589082 |0 (DE-627)691434549 |0 (DE-576)289965233 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Heart rhythm |d New York, NY [u.a.] : Elsevier, 2004 |g 9(2012), 2, Seite 265-272 |h Online-Ressource |w (DE-627)391333739 |w (DE-600)2152161-X |w (DE-576)309205557 |x 1556-3871 |7 nnas |a Genetic suppression of atrial fibrillation using a dominant-negative ether-a-go-go-related gene mutant |
| 773 | 1 | 8 | |g volume:9 |g year:2012 |g number:2 |g pages:265-272 |g extent:8 |a Genetic suppression of atrial fibrillation using a dominant-negative ether-a-go-go-related gene mutant |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.hrthm.2011.09.008 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S1547527111010459 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180802 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 115589082 |a Bauer, Alexander |m 115589082:Bauer, Alexander |d 50000 |e 50000PB115589082 |k 0/50000/ |p 10 |y j | ||
| 998 | |g 108916618 |a Katus, Hugo |m 108916618:Katus, Hugo |d 910000 |d 910100 |e 910000PK108916618 |e 910100PK108916618 |k 0/910000/ |k 1/910000/910100/ |p 9 | ||
| 998 | |g 1059101904 |a Koenen, Michael |m 1059101904:Koenen, Michael |p 8 | ||
| 998 | |g 1032502541 |a Becker, Rüdiger |m 1032502541:Becker, Rüdiger |d 910000 |d 910100 |e 910000PB1032502541 |e 910100PB1032502541 |k 0/910000/ |k 1/910000/910100/ |p 7 | ||
| 998 | |g 122527364 |a Voss, Frederik |m 122527364:Voss, Frederik |d 910000 |d 910100 |e 910000PV122527364 |e 910100PV122527364 |k 0/910000/ |k 1/910000/910100/ |p 6 | ||
| 998 | |g 13306476X |a Seyler, Claudia |m 13306476X:Seyler, Claudia |d 910000 |d 910100 |e 910000PS13306476X |e 910100PS13306476X |k 0/910000/ |k 1/910000/910100/ |p 5 | ||
| 998 | |g 1024753360 |a Bikou, Olympia |m 1024753360:Bikou, Olympia |d 910000 |d 910100 |e 910000PB1024753360 |e 910100PB1024753360 |k 0/910000/ |k 1/910000/910100/ |p 4 | ||
| 998 | |g 129031933 |a Kelemen, Kamilla |m 129031933:Kelemen, Kamilla |d 910000 |d 910100 |e 910000PK129031933 |e 910100PK129031933 |k 0/910000/ |k 1/910000/910100/ |p 3 | ||
| 998 | |g 123896258 |a Thomas, Dierk |m 123896258:Thomas, Dierk |d 910000 |d 910100 |e 910000PT123896258 |e 910100PT123896258 |k 0/910000/ |k 1/910000/910100/ |p 2 | ||
| 998 | |g 1020322519 |a Soucek, Radim |m 1020322519:Soucek, Radim |d 910000 |d 910100 |e 910000PS1020322519 |e 910100PS1020322519 |k 0/910000/ |k 1/910000/910100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1578206448 |e 3020107253 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Available online 8 September 2011","Gesehen am 02.08.2018"],"name":{"displayForm":["Radim Soucek, MD, Dierk Thomas, MD, Kamilla Kelemen, MD, Olympia Bikou, MD, Claudia Seyler, PhD, Frederik Voss, MD, Rüdiger Becker, MD, Michael Koenen, PhD, Hugo A. Katus, MD, Alexander Bauer, MD"]},"language":["eng"],"title":[{"title":"Genetic suppression of atrial fibrillation using a dominant-negative ether-a-go-go-related gene mutant","title_sort":"Genetic suppression of atrial fibrillation using a dominant-negative ether-a-go-go-related gene mutant"}],"physDesc":[{"extent":"8 S."}],"relHost":[{"type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"391333739","disp":"Genetic suppression of atrial fibrillation using a dominant-negative ether-a-go-go-related gene mutantHeart rhythm","part":{"text":"9(2012), 2, Seite 265-272","year":"2012","extent":"8","issue":"2","volume":"9","pages":"265-272"},"pubHistory":["1.2004 -"],"origin":[{"publisherPlace":"New York, NY [u.a.]","dateIssuedKey":"2004","dateIssuedDisp":"2004-","publisher":"Elsevier"}],"id":{"issn":["1556-3871"],"eki":["391333739"],"zdb":["2152161-X"]},"note":["Gesehen am 01.11.11"],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Heart rhythm","title":"Heart rhythm"}]}],"person":[{"role":"aut","family":"Soucek","display":"Soucek, Radim","given":"Radim"},{"role":"aut","family":"Thomas","display":"Thomas, Dierk","given":"Dierk"},{"role":"aut","family":"Kelemen","display":"Kelemen, Kamilla","given":"Kamilla"},{"given":"Olympia","family":"Bikou","display":"Bikou, Olympia","role":"aut"},{"family":"Seyler","display":"Seyler, Claudia","role":"aut","given":"Claudia"},{"role":"aut","display":"Voss, Frederik","family":"Voss","given":"Frederik"},{"given":"Rüdiger","role":"aut","display":"Becker, Rüdiger","family":"Becker"},{"family":"Koenen","display":"Koenen, Michael","role":"aut","given":"Michael"},{"given":"Hugo","display":"Katus, Hugo","family":"Katus","role":"aut"},{"role":"aut","family":"Bauer","display":"Bauer, Alexander","given":"Alexander"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"origin":[{"dateIssuedKey":"2012","dateIssuedDisp":"2012"}],"recId":"1578206448","id":{"eki":["1578206448"],"doi":["10.1016/j.hrthm.2011.09.008"]}} | ||
| SRT | |a SOUCEKRADIGENETICSUP2012 | ||