One-way allosteric communication between the two disulfide bonds in tissue factor
Tissue factor (TF) is a transmembrane glycoprotein that plays distinct roles in the initiation of extrinsic coagulation cascade and thrombosis. TF contains two disulfide bonds, one each in the N-terminal and C-terminal extracellular domains. The C-domain disulfide, Cys186-Cys209, has a −RHStaple con...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
10 January 2017
|
| In: |
Biophysical journal
Year: 2017, Jahrgang: 112, Heft: 1, Pages: 78-86 |
| ISSN: | 1542-0086 |
| DOI: | 10.1016/j.bpj.2016.12.003 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.bpj.2016.12.003 Verlag, kostenfrei, Volltext: http://www.sciencedirect.com/science/article/pii/S0006349516342771 
|
| Verfasserangaben: | Beifei Zhou, Philip J. Hogg, and Frauke Gräter |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1578303079 | ||
| 003 | DE-627 | ||
| 005 | 20220814210719.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180806s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.bpj.2016.12.003 |2 doi | |
| 035 | |a (DE-627)1578303079 | ||
| 035 | |a (DE-576)508303079 | ||
| 035 | |a (DE-599)BSZ508303079 | ||
| 035 | |a (OCoLC)1341016407 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Zhou, Beifei |e VerfasserIn |0 (DE-588)1163974870 |0 (DE-627)1028369220 |0 (DE-576)508302145 |4 aut | |
| 245 | 1 | 0 | |a One-way allosteric communication between the two disulfide bonds in tissue factor |c Beifei Zhou, Philip J. Hogg, and Frauke Gräter |
| 264 | 1 | |c 10 January 2017 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 06.08.2018 | ||
| 520 | |a Tissue factor (TF) is a transmembrane glycoprotein that plays distinct roles in the initiation of extrinsic coagulation cascade and thrombosis. TF contains two disulfide bonds, one each in the N-terminal and C-terminal extracellular domains. The C-domain disulfide, Cys186-Cys209, has a −RHStaple configuration in crystal structures, suggesting that this disulfide carries high pre-stress. The redox state of this disulfide has been proposed to regulate TF encryption/decryption. Ablating the N-domain Cys49-Cys57 disulfide bond was found to increase the redox potential of the Cys186-Cys209 bond, implying an allosteric communication between the domains. Using molecular dynamics simulations, we observed that the Cys186-Cys209 disulfide bond retained the −RHStaple configuration, whereas the Cys49-Cys57 disulfide bond fluctuated widely. The Cys186-Cys209 bond featured the typical −RHStaple disulfide properties, such as a longer S-S bond length, larger C-S-S angles, and higher bonded prestress, in comparison to the Cys49-Cys57 bond. Force distribution analysis was used to sense the subtle structural changes upon ablating the disulfide bonds, and allowed us to identify a one-way allosteric communication mechanism from the N-terminal to the C-terminal domain. We propose a force propagation pathway using a shortest-pathway algorithm, which we suggest is a useful method for searching allosteric signal transduction pathways in proteins. As a possible explanation for the pathway being one-way, we identified a pronounced lower degree of conformational fluctuation, or effectively higher stiffness, in the N-terminal domain. Thus, the changes of the rigid domain (N-terminal domain) can induce mechanical force propagation to the soft domain (C-terminal domain), but not vice versa. | ||
| 700 | 1 | |a Hogg, Philip J. |e VerfasserIn |0 (DE-588)1163975419 |0 (DE-627)1028370431 |0 (DE-576)508303125 |4 aut | |
| 700 | 1 | |a Gräter, Frauke |e VerfasserIn |0 (DE-588)130664871 |0 (DE-627)505296764 |0 (DE-576)298331314 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biophysical journal |d Cambridge, Mass. : Cell Press, 1960 |g 112(2017), 1, Seite 78-86 |h Online-Ressource |w (DE-627)270425357 |w (DE-600)1477214-0 |w (DE-576)091017602 |x 1542-0086 |7 nnas |a One-way allosteric communication between the two disulfide bonds in tissue factor |
| 773 | 1 | 8 | |g volume:112 |g year:2017 |g number:1 |g pages:78-86 |g extent:9 |a One-way allosteric communication between the two disulfide bonds in tissue factor |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.bpj.2016.12.003 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0006349516342771 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180806 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 130664871 |a Gräter, Frauke |m 130664871:Gräter, Frauke |d 700000 |d 708000 |d 700000 |d 718000 |e 700000PG130664871 |e 708000PG130664871 |e 700000PG130664871 |e 718000PG130664871 |k 0/700000/ |k 1/700000/708000/ |k 0/700000/ |k 1/700000/718000/ |p 3 |y j | ||
| 998 | |g 1163974870 |a Zhou, Beifei |m 1163974870:Zhou, Beifei |p 1 |x j | ||
| 999 | |a KXP-PPN1578303079 |e 3020299101 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1578303079","relHost":[{"name":{"displayForm":["ed. by the Biophysical Society"]},"language":["eng"],"id":{"zdb":["1477214-0"],"eki":["270425357"],"issn":["1542-0086"]},"note":["Gesehen am 20.05.2020"],"origin":[{"dateIssuedKey":"1960","publisher":"Cell Press ; Soc. ; Rockefeller Univ. Press","dateIssuedDisp":"1960-","publisherPlace":"Cambridge, Mass. ; Bethesda, Md. ; New York, NY"}],"pubHistory":["1.1960/61 -"],"recId":"270425357","type":{"media":"Online-Ressource","bibl":"periodical"},"part":{"issue":"1","extent":"9","volume":"112","text":"112(2017), 1, Seite 78-86","pages":"78-86","year":"2017"},"title":[{"subtitle":"BJ","title":"Biophysical journal","title_sort":"Biophysical journal"}],"disp":"One-way allosteric communication between the two disulfide bonds in tissue factorBiophysical journal","physDesc":[{"extent":"Online-Ressource"}],"corporate":[{"display":"Biophysical Society","role":"isb"}],"titleAlt":[{"title":"BJ"}]}],"note":["Gesehen am 06.08.2018"],"origin":[{"dateIssuedKey":"2017","dateIssuedDisp":"10 January 2017"}],"person":[{"role":"aut","given":"Beifei","display":"Zhou, Beifei","family":"Zhou"},{"family":"Hogg","given":"Philip J.","display":"Hogg, Philip J.","role":"aut"},{"family":"Gräter","role":"aut","given":"Frauke","display":"Gräter, Frauke"}],"title":[{"title_sort":"One-way allosteric communication between the two disulfide bonds in tissue factor","title":"One-way allosteric communication between the two disulfide bonds in tissue factor"}],"id":{"eki":["1578303079"],"doi":["10.1016/j.bpj.2016.12.003"]},"physDesc":[{"extent":"9 S."}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"name":{"displayForm":["Beifei Zhou, Philip J. Hogg, and Frauke Gräter"]},"language":["eng"]} | ||
| SRT | |a ZHOUBEIFEIONEWAYALLO1020 | ||