Protocol for efficient generation and characterization of adeno-associated viral vectors
Adeno-associated virus vectors are a powerful tool for gene transfer approaches. We have established a simple and fast plasmid-based production system for achieving high adeno-associated virus titers within 6 working days. The same procedure can be used for all serotypes and thus allows direct compa...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
1 Oct 2017
|
| In: |
Human gene therapy. Methods
Year: 2017, Jahrgang: 28, Heft: 5, Pages: 235-246 |
| ISSN: | 1946-6544 |
| DOI: | 10.1089/hgtb.2017.192 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1089/hgtb.2017.192 Verlag, Volltext: https://www.liebertpub.com/doi/10.1089/hgtb.2017.192 
|
| Verfasserangaben: | Andreas Jungmann, Barbara Leuchs, Jean Rommelaere, Hugo A. Katus, and Oliver J. Müller |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1578484480 | ||
| 003 | DE-627 | ||
| 005 | 20231019134940.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180810s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1089/hgtb.2017.192 |2 doi | |
| 035 | |a (DE-627)1578484480 | ||
| 035 | |a (DE-576)508484480 | ||
| 035 | |a (DE-599)BSZ508484480 | ||
| 035 | |a (OCoLC)1341016785 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Jungmann, Andreas |d 1976- |e VerfasserIn |0 (DE-588)135606454 |0 (DE-627)56859455X |0 (DE-576)300540884 |4 aut | |
| 245 | 1 | 0 | |a Protocol for efficient generation and characterization of adeno-associated viral vectors |c Andreas Jungmann, Barbara Leuchs, Jean Rommelaere, Hugo A. Katus, and Oliver J. Müller |
| 264 | 1 | |c 1 Oct 2017 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 10.08.2018 | ||
| 500 | |a Published Online:1 October 2017 | ||
| 520 | |a Adeno-associated virus vectors are a powerful tool for gene transfer approaches. We have established a simple and fast plasmid-based production system for achieving high adeno-associated virus titers within 6 working days. The same procedure can be used for all serotypes and thus allows direct comparability of different serotypes. In this protocol we describe a step-by-step procedure that results in well-characterized vectors suitable for both in vitro approaches and preclinical studies. | ||
| 700 | 1 | |a Leuchs, Barbara |e VerfasserIn |0 (DE-588)1190055112 |0 (DE-627)1668742330 |4 aut | |
| 700 | 1 | |a Rommelaere, Jean |d 1948- |e VerfasserIn |0 (DE-588)1201673909 |0 (DE-627)1685754767 |4 aut | |
| 700 | 1 | |a Katus, Hugo |d 1951- |e VerfasserIn |0 (DE-588)108916618 |0 (DE-627)577155040 |0 (DE-576)289625076 |4 aut | |
| 700 | 1 | |a Müller, Oliver J. |d 1971- |e VerfasserIn |0 (DE-588)121693449 |0 (DE-627)705619621 |0 (DE-576)292833504 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Human gene therapy. Methods |d New York, NY : Liebert, 2012 |g 28(2017), 5, Seite 235-246 |h Online-Ressource |w (DE-627)686943589 |w (DE-600)2650969-6 |w (DE-576)406861935 |x 1946-6544 |7 nnas |a Protocol for efficient generation and characterization of adeno-associated viral vectors |
| 773 | 1 | 8 | |g volume:28 |g year:2017 |g number:5 |g pages:235-246 |g extent:12 |a Protocol for efficient generation and characterization of adeno-associated viral vectors |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1089/hgtb.2017.192 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://www.liebertpub.com/doi/10.1089/hgtb.2017.192 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180810 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 121693449 |a Müller, Oliver J. |m 121693449:Müller, Oliver J. |d 910000 |d 910100 |d 50000 |e 910000PM121693449 |e 910100PM121693449 |e 50000PM121693449 |k 0/910000/ |k 1/910000/910100/ |k 0/50000/ |p 5 |y j | ||
| 998 | |g 108916618 |a Katus, Hugo |m 108916618:Katus, Hugo |d 910000 |d 910100 |e 910000PK108916618 |e 910100PK108916618 |k 0/910000/ |k 1/910000/910100/ |p 4 | ||
| 998 | |g 1201673909 |a Rommelaere, Jean |m 1201673909:Rommelaere, Jean |d 50000 |e 50000PR1201673909 |k 0/50000/ |p 3 | ||
| 998 | |g 1190055112 |a Leuchs, Barbara |m 1190055112:Leuchs, Barbara |p 2 | ||
| 998 | |g 135606454 |a Jungmann, Andreas |m 135606454:Jungmann, Andreas |d 910000 |d 910100 |e 910000PJ135606454 |e 910100PJ135606454 |k 0/910000/ |k 1/910000/910100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1578484480 |e 302059202X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"extent":"12 S."}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"doi":["10.1089/hgtb.2017.192"],"eki":["1578484480"]},"relHost":[{"disp":"Protocol for efficient generation and characterization of adeno-associated viral vectorsHuman gene therapy. Methods","recId":"686943589","titleAlt":[{"title":"Human gene therapy / Methods"},{"title":"HGT methods"}],"language":["eng"],"pubHistory":["23.2012 -"],"type":{"media":"Online-Ressource","bibl":"periodical"},"origin":[{"dateIssuedKey":"2012","publisherPlace":"New York, NY","publisher":"Liebert","dateIssuedDisp":"2012-"}],"title":[{"title":"Human gene therapy","partname":"Methods","title_sort":"Human gene therapy"}],"id":{"issn":["1946-6544"],"zdb":["2650969-6"],"eki":["686943589"]},"part":{"pages":"235-246","year":"2017","volume":"28","text":"28(2017), 5, Seite 235-246","issue":"5","extent":"12"},"physDesc":[{"extent":"Online-Ressource"}],"note":["23.2012,1 als Pt. B bez","Gesehen am 14.11.18"]}],"note":["Gesehen am 10.08.2018","Published Online:1 October 2017"],"person":[{"family":"Jungmann","display":"Jungmann, Andreas","given":"Andreas","role":"aut"},{"role":"aut","display":"Leuchs, Barbara","given":"Barbara","family":"Leuchs"},{"role":"aut","display":"Rommelaere, Jean","given":"Jean","family":"Rommelaere"},{"family":"Katus","given":"Hugo","display":"Katus, Hugo","role":"aut"},{"family":"Müller","given":"Oliver J.","display":"Müller, Oliver J.","role":"aut"}],"recId":"1578484480","name":{"displayForm":["Andreas Jungmann, Barbara Leuchs, Jean Rommelaere, Hugo A. Katus, and Oliver J. Müller"]},"origin":[{"dateIssuedDisp":"1 Oct 2017","dateIssuedKey":"2017"}],"language":["eng"],"title":[{"title_sort":"Protocol for efficient generation and characterization of adeno-associated viral vectors","title":"Protocol for efficient generation and characterization of adeno-associated viral vectors"}]} | ||
| SRT | |a JUNGMANNANPROTOCOLFO1201 | ||