Long-term in vitro culture of hamster pancreatic β-cells and induction of adenocarcinoma by treatment with N-nitrosobis(2-oxopropyl)amine
Objectives Earlier studies indicated that hamster pancreatic ductal adenocarcinoma not only derives from ductal/ductular structures but also from cells within the islet. So far unidentified cells within the islet are responsive to the carcinogenic effect of N-nitrosobis (2-oxopropyl) amine (BOP) for...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
11 May 2012
|
| In: |
Pancreatology
Year: 2012, Jahrgang: 12, Heft: 4, Pages: 380-387 |
| ISSN: | 1424-3911 |
| DOI: | 10.1016/j.pan.2012.05.004 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1016/j.pan.2012.05.004 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S1424390312001305 
|
| Verfasserangaben: | S.A. Müller, K. Bläuer, G. Ergin, F. Bergmann, K. Z'graggen, B.M. Schmied, A. Ulrich |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1578512875 | ||
| 003 | DE-627 | ||
| 005 | 20250206095946.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180813s2012 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.pan.2012.05.004 |2 doi | |
| 035 | |a (DE-627)1578512875 | ||
| 035 | |a (DE-576)508512875 | ||
| 035 | |a (DE-599)BSZ508512875 | ||
| 035 | |a (OCoLC)1341016511 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Müller, Sascha A. |d 1976- |e VerfasserIn |0 (DE-588)1021104795 |0 (DE-627)691459525 |0 (DE-576)358875757 |4 aut | |
| 245 | 1 | 0 | |a Long-term in vitro culture of hamster pancreatic β-cells and induction of adenocarcinoma by treatment with N-nitrosobis(2-oxopropyl)amine |c S.A. Müller, K. Bläuer, G. Ergin, F. Bergmann, K. Z'graggen, B.M. Schmied, A. Ulrich |
| 246 | 3 | 3 | |a Long-term in vitro culture of hamster pancreatic beta-cells and induction of adenocarcinoma by treatment with N-nitrosobis(2-oxopropyl)amine |
| 264 | 1 | |c 11 May 2012 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 13.08.2018 | ||
| 520 | |a Objectives Earlier studies indicated that hamster pancreatic ductal adenocarcinoma not only derives from ductal/ductular structures but also from cells within the islet. So far unidentified cells within the islet are responsive to the carcinogenic effect of N-nitrosobis (2-oxopropyl) amine (BOP) forming poorly differentiated ductal adenocarcinoma. However, studies indicated a major role of β-cells during carcinogenesis. To find out, if β-cells are the primary target cells of BOP and if they are capable to form ductal adenocarcinoma after malignant transformation, we established a long-term culture of undifferentiated cells deriving from isolated β-cells and treated them with BOP. Methods Langerhans' islets from pancreata of Syrian golden hamsters were isolated and dispersed into single cells by dispase digestion. Cells were labeled with a highly specific β-cell surface antibody (K14D10) and these K14D10+ cells were extracted from the suspension by paramagnetic Dynabeads. Cells were cultured in vitro and treated with BOP. Untreated cells served as control. Results K14D10+ cells formed a monolayer and produced insulin over a period of 28 days in culture. However, with time in culture they became undifferentiated with a higher proliferation rate and after about 60 days in culture BOP treated cells showed anchorage independent growth. These cells autotransplanted s.c. formed a well-differentiated ductal adenocarcinoma. Conclusions Pancreatic β-cells are the primary target of BOP without necessarily being embedded in the compound of the Langerhans' islet. With time in culture, they give rise to undifferentiated cells and after malignant transformation they are able to form ductal adenocarcinoma. | ||
| 650 | 4 | |a -nitrosobis(2-oxopropyl)amine | |
| 650 | 4 | |a culture | |
| 650 | 4 | |a Hamster pancreatic β-cells | |
| 700 | 1 | |a Bläuer, Karin |e VerfasserIn |0 (DE-588)1164400312 |0 (DE-627)1028848099 |0 (DE-576)508511674 |4 aut | |
| 700 | 1 | |a Ergin, Gökhan |e VerfasserIn |0 (DE-588)1164400991 |0 (DE-627)102884879X |0 (DE-576)508512441 |4 aut | |
| 700 | 1 | |a Bergmann, Frank |d 1972- |e VerfasserIn |0 (DE-588)124407900 |0 (DE-627)363326081 |0 (DE-576)294157840 |4 aut | |
| 700 | 1 | |a Z’graggen, Kaspar |e VerfasserIn |0 (DE-588)1084359405 |0 (DE-627)848798708 |0 (DE-576)456306285 |4 aut | |
| 700 | 1 | |a Schmied, Bruno |e VerfasserIn |0 (DE-588)1026855721 |0 (DE-627)727503944 |0 (DE-576)372188486 |4 aut | |
| 700 | 1 | |a Ulrich, Alexis |e VerfasserIn |0 (DE-588)1022952226 |0 (DE-627)717337847 |0 (DE-576)177315083 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Pancreatology |d Amsterdam : Elsevier, 2001 |g 12(2012), 4, Seite 380-387 |h Online-Ressource |w (DE-627)32764432X |w (DE-600)2043694-4 |w (DE-576)110513657 |x 1424-3911 |7 nnas |a Long-term in vitro culture of hamster pancreatic β-cells and induction of adenocarcinoma by treatment with N-nitrosobis(2-oxopropyl)amine |
| 773 | 1 | 8 | |g volume:12 |g year:2012 |g number:4 |g pages:380-387 |g extent:8 |a Long-term in vitro culture of hamster pancreatic β-cells and induction of adenocarcinoma by treatment with N-nitrosobis(2-oxopropyl)amine |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.pan.2012.05.004 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S1424390312001305 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180813 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 1022952226 |a Ulrich, Alexis |m 1022952226:Ulrich, Alexis |d 910000 |d 910200 |e 910000PU1022952226 |e 910200PU1022952226 |k 0/910000/ |k 1/910000/910200/ |p 7 |y j | ||
| 998 | |g 1026855721 |a Schmied, Bruno |m 1026855721:Schmied, Bruno |d 50000 |e 50000PS1026855721 |k 0/50000/ |p 6 | ||
| 998 | |g 124407900 |a Bergmann, Frank |m 124407900:Bergmann, Frank |d 910000 |d 912000 |e 910000PB124407900 |e 912000PB124407900 |k 0/910000/ |k 1/910000/912000/ |p 4 | ||
| 998 | |g 1164400991 |a Ergin, Gökhan |m 1164400991:Ergin, Gökhan |d 910000 |d 910200 |e 910000PE1164400991 |e 910200PE1164400991 |k 0/910000/ |k 1/910000/910200/ |p 3 | ||
| 998 | |g 1021104795 |a Müller, Sascha A. |m 1021104795:Müller, Sascha A. |p 1 |x j | ||
| 999 | |a KXP-PPN1578512875 |e 3020708206 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"doi":["10.1016/j.pan.2012.05.004"],"eki":["1578512875"]},"titleAlt":[{"title":"Long-term in vitro culture of hamster pancreatic beta-cells and induction of adenocarcinoma by treatment with N-nitrosobis(2-oxopropyl)amine"}],"name":{"displayForm":["S.A. Müller, K. Bläuer, G. Ergin, F. Bergmann, K. Z'graggen, B.M. Schmied, A. Ulrich"]},"recId":"1578512875","physDesc":[{"extent":"8 S."}],"title":[{"title_sort":"Long-term in vitro culture of hamster pancreatic β-cells and induction of adenocarcinoma by treatment with N-nitrosobis(2-oxopropyl)amine","title":"Long-term in vitro culture of hamster pancreatic β-cells and induction of adenocarcinoma by treatment with N-nitrosobis(2-oxopropyl)amine"}],"note":["Gesehen am 13.08.2018"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"relHost":[{"pubHistory":["1.2001 -"],"origin":[{"dateIssuedKey":"2001","dateIssuedDisp":"2001-","publisherPlace":"Amsterdam ; Basel","publisher":"Elsevier ; Karger"}],"note":["Gesehen am 09.01.2019"],"language":["eng"],"type":{"media":"Online-Ressource","bibl":"periodical"},"part":{"issue":"4","extent":"8","text":"12(2012), 4, Seite 380-387","pages":"380-387","volume":"12","year":"2012"},"title":[{"title":"Pancreatology","title_sort":"Pancreatology","subtitle":"official journal of the International Association of Pancreatology (IAP) and the European Pancreatic Club (EPC)"}],"id":{"issn":["1424-3911"],"eki":["32764432X"],"zdb":["2043694-4"]},"physDesc":[{"extent":"Online-Ressource"}],"recId":"32764432X","disp":"Long-term in vitro culture of hamster pancreatic β-cells and induction of adenocarcinoma by treatment with N-nitrosobis(2-oxopropyl)aminePancreatology"}],"person":[{"display":"Müller, Sascha A.","role":"aut","given":"Sascha A.","family":"Müller"},{"given":"Karin","role":"aut","family":"Bläuer","display":"Bläuer, Karin"},{"display":"Ergin, Gökhan","family":"Ergin","role":"aut","given":"Gökhan"},{"display":"Bergmann, Frank","family":"Bergmann","given":"Frank","role":"aut"},{"family":"Z’graggen","role":"aut","given":"Kaspar","display":"Z’graggen, Kaspar"},{"given":"Bruno","role":"aut","family":"Schmied","display":"Schmied, Bruno"},{"display":"Ulrich, Alexis","family":"Ulrich","given":"Alexis","role":"aut"}],"origin":[{"dateIssuedDisp":"11 May 2012","dateIssuedKey":"2012"}]} | ||
| SRT | |a MUELLERSASLONGTERMIN1120 | ||