Cover Image

Super-resolution microscopy with very large working distance by means of distributed aperture illumination

The limits of conventional light microscopy (“Abbe-Limit“) depend critically on the numerical aperture (NA) of the objective lens. Imaging at large working distances or a large field-of-view typically requires low NA objectives, thereby reducing the optical resolution to the multi micrometer range....

Full description

Saved in:
Bibliographic Details
Main Authors: Birk, Udo J. (Author) , Hase, Johann von (Author) , Cremer, Christoph (Author)
Format: Article (Journal)
Language:English
Published: 16 June 2017
In: Scientific reports
Year: 2017, Volume: 7
ISSN:2045-2322
DOI:10.1038/s41598-017-03743-4
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1038/s41598-017-03743-4
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41598-017-03743-4
Get full text
Author Notes:Udo Birk, Johann v. Hase & Christoph Cremer
Description
Summary:The limits of conventional light microscopy (“Abbe-Limit“) depend critically on the numerical aperture (NA) of the objective lens. Imaging at large working distances or a large field-of-view typically requires low NA objectives, thereby reducing the optical resolution to the multi micrometer range. Based on numerical simulations of the intensity field distribution, we present an illumination concept for a super-resolution microscope which allows a three dimensional (3D) optical resolution around 150 nm for working distances up to the centimeter regime. In principle, the system allows great flexibility, because the illumination concept can be used to approximate the point-spread-function of conventional microscope optics, with the additional benefit of a customizable pupil function. Compared with the Abbe-limit using an objective lens with such a large working distance, a volume resolution enhancement potential in the order of 104 is estimated.
Item Description:Published online: 16 June 2017
Gesehen am 14.08.2018
Physical Description:Online Resource
ISSN:2045-2322
DOI:10.1038/s41598-017-03743-4

Similar Items