Testing for antibodies to human aquaporin-4 by ELISA: sensitivity, specificity, and direct comparison with immunohistochemistry
Background Several assays have been developed to detect antibodies to aquaporin-4 (NMO-IgG/AQP4-Ab). However, many of these assays require sophisticated techniques and are thus only available at specialized laboratories. This is problematic since NMO-IgG/AQP4-Ab testing has important prognostic and...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
16 June 2012
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| In: |
Journal of the neurological sciences
Year: 2012, Volume: 320, Issue: 1, Pages: 32-37 |
| ISSN: | 1878-5883 |
| DOI: | 10.1016/j.jns.2012.06.002 |
| Online Access: | Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0022510X12002857 Verlag, Volltext: http://dx.doi.org/10.1016/j.jns.2012.06.002 
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| Author Notes: | S. Jarius, D. Franciotta, F. Paul, R. Bergamaschi, P. S. Rommer, K. Ruprecht, M. Ringelstein, O. Aktas, W. Kristoferitsch, B. Wildemann |
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| 520 | |a Background Several assays have been developed to detect antibodies to aquaporin-4 (NMO-IgG/AQP4-Ab). However, many of these assays require sophisticated techniques and are thus only available at specialized laboratories. This is problematic since NMO-IgG/AQP4-Ab testing has important prognostic and therapeutic implications. Objective To evaluate a newly developed, commercial, enzyme-linked immunosorbent assay (ELISA) for detecting NMO-IgG/AQP4-Ab. Methods Serum samples from 261 patients with NMO spectrum disorders (NMOSD; n=108) and controls (n=153) were tested for AQP4-Ab by using ELISA. Of these patients, 207 were tested in parallel using a standard immunohistochemical (IHC) assay. Results Fifty of 66 (75.8%) patients with NMO, 17/25 (68%) with LETM, 3/14 (21.4%) with ON, 2/3 (66.7%) with ON and non-extensive transverse myelitis, and 2/153 (1.3%) controls tested positive in the ELISA. Of those NMOSD patients tested by both ELISA and IHC, 10 were positive only in the ELISA and 3 exclusively in the IHC assay, suggesting that the overall sensitivity of the ELISA was higher than that of the standard IHC assay. The ELISA yielded very good intra- and inter-run reproducibility with regard to AQP4-Ab detection and good intrarun, but only moderate inter-run reproducibility with regard to AQP4-Ab quantification. Anti-AQP4 serum concentrations correlated with disease activity (p<0.00001), but did not differ between patients with NMO and patients with isolated LETM or ON. Conclusion The ELISA evaluated here provides a relatively sensitive and easy-to-use diagnostic tool for detecting antibodies to AQP4 and could make AQP4-Ab testing, which is of high clinical relevance, more widely available. | ||
| 650 | 4 | |a Antibody to aquaporin-4 | |
| 650 | 4 | |a Diagnosis | |
| 650 | 4 | |a Enzyme-linked immunosorbent assay (ELISA) | |
| 650 | 4 | |a Indirect immunofluorescence | |
| 650 | 4 | |a Longitudinally extensive transverse myelitis | |
| 650 | 4 | |a Multiple sclerosis | |
| 650 | 4 | |a Neuromyelitis optica (Devic's syndrome) | |
| 650 | 4 | |a NMO-IgG | |
| 650 | 4 | |a Optic neuritis | |
| 650 | 4 | |a Recombinant antigen | |
| 700 | 1 | |a Wildemann, Brigitte |e VerfasserIn |0 (DE-588)110203844 |0 (DE-627)510150004 |0 (DE-576)171831330 |4 aut | |
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