Expression of the RNA-binding protein RBP10 promotes the bloodstream-form differentiation state in Trypanosoma brucei
In nearly all eukaryotes, cellular differentiation is governed by changes in transcription, and stabilized by chromatin and DNA modification. Gene expression control in the pathogen Trypanosoma brucei, in contrast, relies almost exclusively on post-transcriptional mechanisms, so RNA binding proteins...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
August 11, 2017
|
| In: |
PLoS pathogens
Year: 2017, Jahrgang: 13, Heft: 8 |
| ISSN: | 1553-7374 |
| DOI: | 10.1371/journal.ppat.1006560 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1371/journal.ppat.1006560 Verlag, kostenfrei, Volltext: http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006560 
|
| Verfasserangaben: | Elisha Mugo, Christine Clayton |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1580268927 | ||
| 003 | DE-627 | ||
| 005 | 20220814225821.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180822s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.ppat.1006560 |2 doi | |
| 035 | |a (DE-627)1580268927 | ||
| 035 | |a (DE-576)510268927 | ||
| 035 | |a (DE-599)BSZ510268927 | ||
| 035 | |a (OCoLC)1341017436 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Mugo, Elisha Muchunga |e VerfasserIn |0 (DE-588)1138933651 |0 (DE-627)89677354X |0 (DE-576)492767831 |4 aut | |
| 245 | 1 | 0 | |a Expression of the RNA-binding protein RBP10 promotes the bloodstream-form differentiation state in Trypanosoma brucei |c Elisha Mugo, Christine Clayton |
| 264 | 1 | |c August 11, 2017 | |
| 300 | |a 37 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 22.08.2018 | ||
| 520 | |a In nearly all eukaryotes, cellular differentiation is governed by changes in transcription, and stabilized by chromatin and DNA modification. Gene expression control in the pathogen Trypanosoma brucei, in contrast, relies almost exclusively on post-transcriptional mechanisms, so RNA binding proteins must assume the burden that is usually borne by transcription factors. T. brucei multiply in the blood of mammals as bloodstream forms, and in the midgut of Tsetse flies as procyclic forms. We show here that a single RNA-binding protein, RBP10, promotes the bloodstream-form trypanosome differentiation state. Depletion of RBP10 from bloodstream-form trypanosomes gives cells that can grow only as procyclic forms; conversely, expression of RBP10 in procyclic forms converts them to bloodstream forms. RBP10 binds to procyclic-specific mRNAs containing an UAUUUUUU motif, targeting them for translation repression and destruction. Products of RBP10 target mRNAs include not only the major procyclic surface protein and enzymes of energy metabolism, but also protein kinases and stage-specific RNA-binding proteins: this suggests that alterations in RBP10 trigger a regulatory cascade. | ||
| 650 | 4 | |a Cell differentiation | |
| 650 | 4 | |a Epimastigotes | |
| 650 | 4 | |a Messenger RNA | |
| 650 | 4 | |a Protein translation | |
| 650 | 4 | |a RNA interference | |
| 650 | 4 | |a RNA-binding proteins | |
| 650 | 4 | |a Sequence motif analysis | |
| 650 | 4 | |a Trypanosoma | |
| 700 | 1 | |a Clayton, Christine |e VerfasserIn |0 (DE-588)1098447158 |0 (DE-627)85763335X |0 (DE-576)469125624 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a Public Library of Science |t PLoS pathogens |d Lawrence, Kan. : PLoS, 2005 |g 13(2017,8) Artikel-Nummer e1006560, 37 Seiten |h Online-Ressource |w (DE-627)501074422 |w (DE-600)2205412-1 |w (DE-576)273890530 |x 1553-7374 |7 nnas |
| 773 | 1 | 8 | |g volume:13 |g year:2017 |g number:8 |g extent:37 |a Expression of the RNA-binding protein RBP10 promotes the bloodstream-form differentiation state in Trypanosoma brucei |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1371/journal.ppat.1006560 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006560 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180822 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 1098447158 |a Clayton, Christine |m 1098447158:Clayton, Christine |d 700000 |d 706000 |d 700000 |d 718000 |e 700000PC1098447158 |e 706000PC1098447158 |e 700000PC1098447158 |e 718000PC1098447158 |k 0/700000/ |k 1/700000/706000/ |k 0/700000/ |k 1/700000/718000/ |p 2 |y j | ||
| 998 | |g 1138933651 |a Mugo, Elisha Muchunga |m 1138933651:Mugo, Elisha Muchunga |d 700000 |d 706000 |e 700000PM1138933651 |e 706000PM1138933651 |k 0/700000/ |k 1/700000/706000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1580268927 |e 3022918143 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedKey":"2017","dateIssuedDisp":"August 11, 2017"}],"id":{"doi":["10.1371/journal.ppat.1006560"],"eki":["1580268927"]},"name":{"displayForm":["Elisha Mugo, Christine Clayton"]},"physDesc":[{"extent":"37 S."}],"relHost":[{"id":{"zdb":["2205412-1"],"eki":["501074422"],"issn":["1553-7374"]},"origin":[{"publisherPlace":"Lawrence, Kan.","dateIssuedKey":"2005","publisher":"PLoS","dateIssuedDisp":"2005-"}],"name":{"displayForm":["Public Library of Science"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"PLoS pathogens","title_sort":"PLoS pathogens"}],"titleAlt":[{"title":"Pathogens"}],"part":{"year":"2017","issue":"8","text":"13(2017,8) Artikel-Nummer e1006560, 37 Seiten","volume":"13","extent":"37"},"pubHistory":["1.2005,Sept. -"],"recId":"501074422","corporate":[{"display":"Public Library of Science","roleDisplay":"VerfasserIn","role":"aut"}],"language":["eng"],"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Public Library of SciencePLoS pathogens","note":["Gesehen am 20.11.2020"]}],"title":[{"title_sort":"Expression of the RNA-binding protein RBP10 promotes the bloodstream-form differentiation state in Trypanosoma brucei","title":"Expression of the RNA-binding protein RBP10 promotes the bloodstream-form differentiation state in Trypanosoma brucei"}],"person":[{"given":"Elisha Muchunga","family":"Mugo","role":"aut","roleDisplay":"VerfasserIn","display":"Mugo, Elisha Muchunga"},{"family":"Clayton","given":"Christine","display":"Clayton, Christine","roleDisplay":"VerfasserIn","role":"aut"}],"note":["Gesehen am 22.08.2018"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"recId":"1580268927","language":["eng"]} | ||
| SRT | |a MUGOELISHAEXPRESSION1120 | ||