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Quantitative live-cell imaging of human immunodeficiency virus (HIV-1) assembly

Advances in fluorescence methodologies make it possible to investigate biological systems in unprecedented detail. Over the last few years, quantitative live-cell imaging has increasingly been used to study the dynamic interactions of viruses with cells and is expected to become even more indispensa...

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Hauptverfasser: Baumgärtel, Viola (VerfasserIn) , Müller, Barbara (VerfasserIn) , Lamb, Don C. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 4 May 2012
In: Viruses
Year: 2012, Jahrgang: 4, Heft: 5, Pages: 777-799
ISSN:1999-4915
DOI:10.3390/v4050777
Online-Zugang:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.3390/v4050777
Verlag, kostenfrei, Volltext: http://www.mdpi.com/1999-4915/4/5/777
Volltext
Verfasserangaben:Viola Baumgärtel, Barbara Müller, Don C. Lamb, Viola Baumgärtel, Barbara Müller and Don C. Lamb

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520 |a Advances in fluorescence methodologies make it possible to investigate biological systems in unprecedented detail. Over the last few years, quantitative live-cell imaging has increasingly been used to study the dynamic interactions of viruses with cells and is expected to become even more indispensable in the future. Here, we describe different fluorescence labeling strategies that have been used to label HIV-1 for live cell imaging and the fluorescence based methods used to visualize individual aspects of virus-cell interactions. This review presents an overview of experimental methods and recent experiments that have employed quantitative microscopy in order to elucidate the dynamics of late stages in the HIV-1 replication cycle. This includes cytosolic interactions of the main structural protein, Gag, with itself and the viral RNA genome, the recruitment of Gag and RNA to the plasma membrane, virion assembly at the membrane and the recruitment of cellular proteins involved in HIV-1 release to the nascent budding site. 
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