Association of peripheral NK cell counts with Helios+IFN-γ- T regs in patients with good long-term renal allograft function
Little is known about a possible interaction of natural killer (NK) cells with regulatory T cells (Treg) in long-term stable kidney transplant recipients. Absolute counts of lymphocyte and Treg subsets were studied in whole blood samples of 136 long-term stable renal transplant recipients and 52 hea...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
14 February 2017
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| In: |
Clinical & experimental immunology
Year: 2017, Jahrgang: 188, Heft: 3, Pages: 467-479 |
| ISSN: | 1365-2249 |
| DOI: | 10.1111/cei.12945 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1111/cei.12945 Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/cei.12945 
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| Verfasserangaben: | K. Trojan, L. Zhu, M. Aly, R. Weimer, N. Bulut, C. Morath, G. Opelz and V. Daniel |
| Zusammenfassung: | Little is known about a possible interaction of natural killer (NK) cells with regulatory T cells (Treg) in long-term stable kidney transplant recipients. Absolute counts of lymphocyte and Treg subsets were studied in whole blood samples of 136 long-term stable renal transplant recipients and 52 healthy controls using eight-colour fluorescence flow cytometry. Patients were 1946 ± 2201 days (153-10 268 days) post-transplant and showed a serum creatinine of 1·7 ± 0·7 mg/dl. Renal transplant recipients investigated > 1·5 years post-transplant showed higher total NK cell counts than recipients studied < 1·5 years after transplantation (P = 0·006). High NK cells were associated with high glomerular filtration rate (P = 0·002) and low serum creatinine (P = 0·005). Interestingly, high NK cells were associated with high CD4+CD25+CD127-forkhead box protein 3 (FoxP3+) Treg that co-express the phenotype Helios+interferon (IFN)-γ- and appear to have stable FoxP3 expression and originate from the thymus. Furthermore, high total NK cells were associated with Treg that co-express the phenotypes interleukin (IL)−10-transforming growth factor (TGF)-β+ (P = 0·013), CD183+CD62L- (P = 0·003), CD183+CD62+(P = 0·001), CD183-CD62L+ (P = 0·002), CD252-CD152+ (P < 0·001), CD28+human leucocyte antigen D-related (HLA-DR-) (P = 0·002), CD28+HLA-DR+ (P < 0·001), CD95+CD178- (P < 0·001) and CD279-CD152+ (P < 0·001), suggesting that these activated Treg home in peripheral tissues and suppress effector cells via TGF-β and cytotoxic T lymphocyte-associated protein 4 (CTLA-4). The higher numbers of NK and Treg cell counts in patients with long-term good allograft function and the statistical association of these two lymphocyte subsets with each other suggest a direct or indirect (via DC) interaction of these cell subpopulations that contributes to good long-term allograft acceptance. Moreover, we speculate that regulatory NK cells are formed late post-transplant that are able to inhibit graft-reactive effector cells. |
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| Beschreibung: | Gesehen am 03.09.2018 Im Titel sind die Zeichen "+" und "-" hoch und "regs" tief gestellt |
| Beschreibung: | Online Resource |
| ISSN: | 1365-2249 |
| DOI: | 10.1111/cei.12945 |