Cover Image

Mutual recognition of sphingolipid molecular species in membranes

In membranes liquid disordered (ld) and liquid ordered (lo) domains can exist that differ in fluidity and function. Lo areas are predominantly composed of cholesterol and sphingomyelin (SM). Study of the formation of such domains is hampered by a lack of methods to analyze specific lipid-lipid inter...

Full description

Saved in:
Bibliographic Details
Main Authors: Ernst, Andreas M. (Author) , Contreras, F. Xabier (Author) , Wieland, Felix T. (Author) , Brügger, Britta (Author)
Format: Article (Journal)
Language:English
Published: 12 June 2012
In: Biochimica et biophysica acta. Biomembranes
Year: 2012, Volume: 1818, Issue: 11, Pages: 2616-2622
ISSN:1879-2642
DOI:10.1016/j.bbamem.2012.06.004
Online Access:Verlag, Volltext: http://dx.doi.org/10.1016/j.bbamem.2012.06.004
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0005273612001927
Get full text
Author Notes:Andreas Max Ernst, Francesc-Xabier Contreras, Christoph Thiele, Felix Wieland, Britta Brügger

MARC

LEADER 00000caa a2200000 c 4500
001 1580640265
003 DE-627
005 20220815001208.0
007 cr uuu---uuuuu
008 180903s2012 xx |||||o 00| ||eng c
024 7 |a 10.1016/j.bbamem.2012.06.004  |2 doi 
035 |a (DE-627)1580640265 
035 |a (DE-576)510640265 
035 |a (DE-599)BSZ510640265 
035 |a (OCoLC)1341017991 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Ernst, Andreas M.  |e VerfasserIn  |0 (DE-588)1035799960  |0 (DE-627)749589884  |0 (DE-576)383562503  |4 aut 
245 1 0 |a Mutual recognition of sphingolipid molecular species in membranes  |c Andreas Max Ernst, Francesc-Xabier Contreras, Christoph Thiele, Felix Wieland, Britta Brügger 
264 1 |c 12 June 2012 
300 |a 7 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 03.09.2018 
520 |a In membranes liquid disordered (ld) and liquid ordered (lo) domains can exist that differ in fluidity and function. Lo areas are predominantly composed of cholesterol and sphingomyelin (SM). Study of the formation of such domains is hampered by a lack of methods to analyze specific lipid-lipid interactions at low concentrations of individual molecular lipid species in membranes. Here, we developed a simple biophysical method to experimentally assess the affinity of various molecular species of SM for cholesterol, and for their endogenous counterparts (kin) at physiological concentrations. Fluorescent SM (flc SM) molecular species with a conjugated pentaene system in their fatty acids are employed to monitor their affinity to either cholesterol or their kin by fluorescence unquenching. With this novel method we show that specific interactions of individual SMs with cholesterol or their kin exist, indicating the presence of SM nano‐domains in ld-phases, strictly based on kin/cholesterol recognition. 
650 4 |a Cholesterol 
650 4 |a Fluorescent polyene-lipid 
650 4 |a Kin recognition 
650 4 |a Lipid-Lipid Interaction 
650 4 |a Nano-domain 
650 4 |a Sphingomyelin 
700 1 |a Contreras, F. Xabier  |e VerfasserIn  |0 (DE-588)1156239907  |0 (DE-627)1018704310  |0 (DE-576)502053208  |4 aut 
700 1 |a Wieland, Felix T.  |d 1948-  |e VerfasserIn  |0 (DE-588)1020541881  |0 (DE-627)69125365X  |0 (DE-576)360684009  |4 aut 
700 1 |a Brügger, Britta  |d 1969-  |e VerfasserIn  |0 (DE-588)121046028  |0 (DE-627)081051743  |0 (DE-576)292514492  |4 aut 
773 0 8 |i Enthalten in  |t Biochimica et biophysica acta. Biomembranes  |d Amsterdam : Elsevier, 1967  |g 1818(2012), 11, Seite 2616-2622  |w (DE-627)502923369  |w (DE-600)2209384-9  |w (DE-576)251822656  |x 1879-2642  |7 nnas  |a Mutual recognition of sphingolipid molecular species in membranes 
773 1 8 |g volume:1818  |g year:2012  |g number:11  |g pages:2616-2622  |g extent:7  |a Mutual recognition of sphingolipid molecular species in membranes 
856 4 0 |u http://dx.doi.org/10.1016/j.bbamem.2012.06.004  |x Verlag  |x Resolving-System  |3 Volltext 
856 4 0 |u http://www.sciencedirect.com/science/article/pii/S0005273612001927  |x Verlag  |3 Volltext 
951 |a AR 
992 |a 20180903 
993 |a Article 
994 |a 2012 
998 |g 121046028  |a Brügger, Britta  |m 121046028:Brügger, Britta  |d 700000  |d 711000  |d 700000  |d 718000  |e 700000PB121046028  |e 711000PB121046028  |e 700000PB121046028  |e 718000PB121046028  |k 0/700000/  |k 1/700000/711000/  |k 0/700000/  |k 1/700000/718000/  |p 5  |y j 
998 |g 1020541881  |a Wieland, Felix T.  |m 1020541881:Wieland, Felix T.  |d 700000  |d 711000  |d 700000  |d 718000  |e 700000PW1020541881  |e 711000PW1020541881  |e 700000PW1020541881  |e 718000PW1020541881  |k 0/700000/  |k 1/700000/711000/  |k 0/700000/  |k 1/700000/718000/  |p 4 
998 |g 1156239907  |a Contreras, F. Xabier  |m 1156239907:Contreras, F. Xabier  |d 700000  |d 711000  |e 700000PC1156239907  |e 711000PC1156239907  |k 0/700000/  |k 1/700000/711000/  |p 2 
998 |g 1035799960  |a Ernst, Andreas M.  |m 1035799960:Ernst, Andreas M.  |p 1  |x j 
999 |a KXP-PPN1580640265  |e 3024668829 
BIB |a Y 
SER |a journal 
JSO |a {"title":[{"title_sort":"Mutual recognition of sphingolipid molecular species in membranes","title":"Mutual recognition of sphingolipid molecular species in membranes"}],"language":["eng"],"id":{"eki":["1580640265"],"doi":["10.1016/j.bbamem.2012.06.004"]},"physDesc":[{"extent":"7 S."}],"relHost":[{"origin":[{"publisherPlace":"Amsterdam","dateIssuedDisp":"1967-","publisher":"Elsevier","dateIssuedKey":"1967"}],"recId":"502923369","titleAlt":[{"title":"Biochimica et biophysica acta / Biomembranes"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 08.01.2020","Bis 2016 Bände zugleich Bände von: Biochimica et biophysica acta"],"disp":"Mutual recognition of sphingolipid molecular species in membranesBiochimica et biophysica acta. Biomembranes","id":{"eki":["502923369"],"zdb":["2209384-9"],"issn":["1879-2642"]},"pubHistory":["135.1967 -"],"language":["eng"],"part":{"year":"2012","text":"1818(2012), 11, Seite 2616-2622","volume":"1818","issue":"11","extent":"7","pages":"2616-2622"},"title":[{"partname":"Biomembranes","title":"Biochimica et biophysica acta","subtitle":"BBA","title_sort":"Biochimica et biophysica acta"}]}],"note":["Gesehen am 03.09.2018"],"origin":[{"dateIssuedKey":"2012","dateIssuedDisp":"12 June 2012"}],"recId":"1580640265","type":{"media":"Online-Ressource","bibl":"article-journal"},"person":[{"display":"Ernst, Andreas M.","given":"Andreas M.","role":"aut","family":"Ernst"},{"family":"Contreras","display":"Contreras, F. Xabier","given":"F. Xabier","role":"aut"},{"display":"Wieland, Felix T.","given":"Felix T.","role":"aut","family":"Wieland"},{"family":"Brügger","role":"aut","given":"Britta","display":"Brügger, Britta"}],"name":{"displayForm":["Andreas Max Ernst, Francesc-Xabier Contreras, Christoph Thiele, Felix Wieland, Britta Brügger"]}} 
SRT |a ERNSTANDREMUTUALRECO1220 

Similar Items