Efficient single-copy HDR by 5' modified long dsDNA donors
CRISPR/Cas9 efficiently induces targeted mutations via non-homologous-end-joining but for genome editing, precise, homology-directed repair (HDR) of endogenous DNA stretches is a prerequisite. To favor HDR, many approaches interfere with the repair machinery or manipulate Cas9 itself. Using Medaka w...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
Aug 29, 2018
|
| In: |
eLife
Year: 2018, Jahrgang: 7 |
| ISSN: | 2050-084X |
| DOI: | 10.7554/eLife.39468 |
| Online-Zugang: | Resolving-System, kostenfrei, Volltext: http://dx.doi.org/10.7554/eLife.39468 Verlag, kostenfrei, Volltext: https://elifesciences.org/articles/39468 
|
| Verfasserangaben: | Jose Arturo Gutierrez-Triana, Tinatini Tavhelidse, Thomas Thumberger, Isabelle Thomas, Beate Wittbrodt, Tanja Kellner, Kerim Anlas, Erika Tsingos and Joachim Wittbrodt |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1580668895 | ||
| 003 | DE-627 | ||
| 005 | 20220815001812.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180904s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.7554/eLife.39468 |2 doi | |
| 035 | |a (DE-627)1580668895 | ||
| 035 | |a (DE-576)510668895 | ||
| 035 | |a (DE-599)BSZ510668895 | ||
| 035 | |a (OCoLC)1341018171 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Gutiérrez Triana, José Arturo |d 1971- |e VerfasserIn |0 (DE-588)130013536 |0 (DE-627)487571851 |0 (DE-576)297951017 |4 aut | |
| 245 | 1 | 0 | |a Efficient single-copy HDR by 5' modified long dsDNA donors |c Jose Arturo Gutierrez-Triana, Tinatini Tavhelidse, Thomas Thumberger, Isabelle Thomas, Beate Wittbrodt, Tanja Kellner, Kerim Anlas, Erika Tsingos and Joachim Wittbrodt |
| 264 | 1 | |c Aug 29, 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 04.09.2018 | ||
| 520 | |a CRISPR/Cas9 efficiently induces targeted mutations via non-homologous-end-joining but for genome editing, precise, homology-directed repair (HDR) of endogenous DNA stretches is a prerequisite. To favor HDR, many approaches interfere with the repair machinery or manipulate Cas9 itself. Using Medaka we show that the modification of 5’ ends of long dsDNA donors strongly enhances HDR, favors efficient single-copy integration by retaining a monomeric donor conformation thus facilitating successful gene replacement or tagging. | ||
| 700 | 1 | |a Tavhelidse-Suck, Tinatini |e VerfasserIn |0 (DE-588)1124483764 |0 (DE-627)878199837 |0 (DE-576)482786531 |4 aut | |
| 700 | 1 | |a Thumberger, Thomas |e VerfasserIn |0 (DE-588)1026677068 |0 (DE-627)727045091 |0 (DE-576)354676075 |4 aut | |
| 700 | 1 | |a Thomas, Isabelle |e VerfasserIn |0 (DE-588)1166184307 |0 (DE-627)1030132194 |0 (DE-576)510670431 |4 aut | |
| 700 | 1 | |a Wittbrodt, Beate |e VerfasserIn |0 (DE-588)1124418253 |0 (DE-627)878099751 |0 (DE-576)482504056 |4 aut | |
| 700 | 1 | |a Kellner, Tanja |e VerfasserIn |0 (DE-588)1124863559 |0 (DE-627)879196548 |0 (DE-576)483202142 |4 aut | |
| 700 | 1 | |a Anlas, Kerim |e VerfasserIn |0 (DE-588)1166185850 |0 (DE-627)1030133271 |0 (DE-576)510671438 |4 aut | |
| 700 | 1 | |a Tsingos, Erika |d 1990- |e VerfasserIn |0 (DE-588)1161195165 |0 (DE-627)1024610616 |0 (DE-576)506401243 |4 aut | |
| 700 | 1 | |a Wittbrodt, Joachim |e VerfasserIn |0 (DE-588)1038250919 |0 (DE-627)756835356 |0 (DE-576)167753401 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t eLife |d Cambridge : eLife Sciences Publications, 2012 |g 7(2018) Artikel-Nummer e39468, 41 Seiten |h Online-Ressource |w (DE-627)728518384 |w (DE-600)2687154-3 |w (DE-576)372567576 |x 2050-084X |7 nnas |a Efficient single-copy HDR by 5' modified long dsDNA donors |
| 773 | 1 | 8 | |g volume:7 |g year:2018 |a Efficient single-copy HDR by 5' modified long dsDNA donors |
| 856 | 4 | 0 | |u http://dx.doi.org/10.7554/eLife.39468 |x Resolving-System |x Verlag |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://elifesciences.org/articles/39468 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180904 | ||
| 993 | |a Article | ||
| 994 | |a 2018 | ||
| 998 | |g 1038250919 |a Wittbrodt, Joachim |m 1038250919:Wittbrodt, Joachim |d 700000 |d 721000 |e 700000PW1038250919 |e 721000PW1038250919 |k 0/700000/ |k 1/700000/721000/ |p 9 |y j | ||
| 998 | |g 1161195165 |a Tsingos, Erika |m 1161195165:Tsingos, Erika |d 700000 |d 721000 |e 700000PT1161195165 |e 721000PT1161195165 |k 0/700000/ |k 1/700000/721000/ |p 8 | ||
| 998 | |g 1166185850 |a Anlas, Kerim |m 1166185850:Anlas, Kerim |d 700000 |d 721000 |e 700000PA1166185850 |e 721000PA1166185850 |k 0/700000/ |k 1/700000/721000/ |p 7 | ||
| 998 | |g 1124863559 |a Kellner, Tanja |m 1124863559:Kellner, Tanja |d 700000 |d 721000 |e 700000PK1124863559 |e 721000PK1124863559 |k 0/700000/ |k 1/700000/721000/ |p 6 | ||
| 998 | |g 1124418253 |a Wittbrodt, Beate |m 1124418253:Wittbrodt, Beate |d 700000 |d 721000 |e 700000PW1124418253 |e 721000PW1124418253 |k 0/700000/ |k 1/700000/721000/ |p 5 | ||
| 998 | |g 1166184307 |a Thomas, Isabelle |m 1166184307:Thomas, Isabelle |d 700000 |d 721000 |e 700000PT1166184307 |e 721000PT1166184307 |k 0/700000/ |k 1/700000/721000/ |p 4 | ||
| 998 | |g 1026677068 |a Thumberger, Thomas |m 1026677068:Thumberger, Thomas |d 700000 |d 721000 |e 700000PT1026677068 |e 721000PT1026677068 |k 0/700000/ |k 1/700000/721000/ |p 3 | ||
| 998 | |g 1124483764 |a Tavhelidse-Suck, Tinatini |m 1124483764:Tavhelidse-Suck, Tinatini |d 700000 |d 721000 |e 700000PT1124483764 |e 721000PT1124483764 |k 0/700000/ |k 1/700000/721000/ |p 2 | ||
| 998 | |g 130013536 |a Gutiérrez Triana, José Arturo |m 130013536:Gutiérrez Triana, José Arturo |p 1 |x j | ||
| 999 | |a KXP-PPN1580668895 |e 302471216X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 04.09.2018"],"name":{"displayForm":["Jose Arturo Gutierrez-Triana, Tinatini Tavhelidse, Thomas Thumberger, Isabelle Thomas, Beate Wittbrodt, Tanja Kellner, Kerim Anlas, Erika Tsingos and Joachim Wittbrodt"]},"language":["eng"],"title":[{"title":"Efficient single-copy HDR by 5' modified long dsDNA donors","title_sort":"Efficient single-copy HDR by 5' modified long dsDNA donors"}],"relHost":[{"note":["Gesehen am 28.06.17"],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"eLife","title_sort":"eLife"}],"origin":[{"publisherPlace":"Cambridge","dateIssuedKey":"2012","dateIssuedDisp":"2012-","publisher":"eLife Sciences Publications"}],"id":{"eki":["728518384"],"zdb":["2687154-3"],"issn":["2050-084X"]},"part":{"volume":"7","text":"7(2018) Artikel-Nummer e39468, 41 Seiten","year":"2018"},"disp":"Efficient single-copy HDR by 5' modified long dsDNA donorseLife","pubHistory":["1.2012 -"],"titleAlt":[{"title":"eLife journal"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"recId":"728518384"}],"person":[{"given":"José Arturo","display":"Gutiérrez Triana, José Arturo","family":"Gutiérrez Triana","role":"aut"},{"given":"Tinatini","display":"Tavhelidse-Suck, Tinatini","family":"Tavhelidse-Suck","role":"aut"},{"given":"Thomas","family":"Thumberger","display":"Thumberger, Thomas","role":"aut"},{"given":"Isabelle","display":"Thomas, Isabelle","family":"Thomas","role":"aut"},{"given":"Beate","family":"Wittbrodt","display":"Wittbrodt, Beate","role":"aut"},{"given":"Tanja","display":"Kellner, Tanja","family":"Kellner","role":"aut"},{"given":"Kerim","family":"Anlas","display":"Anlas, Kerim","role":"aut"},{"display":"Tsingos, Erika","family":"Tsingos","role":"aut","given":"Erika"},{"given":"Joachim","role":"aut","display":"Wittbrodt, Joachim","family":"Wittbrodt"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"origin":[{"dateIssuedDisp":"Aug 29, 2018","dateIssuedKey":"2018"}],"recId":"1580668895","id":{"doi":["10.7554/eLife.39468"],"eki":["1580668895"]}} | ||
| SRT | |a GUTIERREZTEFFICIENTS2920 | ||