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Efficient single-copy HDR by 5' modified long dsDNA donors

CRISPR/Cas9 efficiently induces targeted mutations via non-homologous-end-joining but for genome editing, precise, homology-directed repair (HDR) of endogenous DNA stretches is a prerequisite. To favor HDR, many approaches interfere with the repair machinery or manipulate Cas9 itself. Using Medaka w...

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Main Authors: Gutiérrez Triana, José Arturo (Author) , Tavhelidse-Suck, Tinatini (Author) , Thumberger, Thomas (Author) , Thomas, Isabelle (Author) , Wittbrodt, Beate (Author) , Kellner, Tanja (Author) , Anlas, Kerim (Author) , Tsingos, Erika (Author) , Wittbrodt, Joachim (Author)
Format: Article (Journal)
Language:English
Published: Aug 29, 2018
In: eLife
Year: 2018, Volume: 7
ISSN:2050-084X
DOI:10.7554/eLife.39468
Online Access:Resolving-System, kostenfrei, Volltext: http://dx.doi.org/10.7554/eLife.39468
Verlag, kostenfrei, Volltext: https://elifesciences.org/articles/39468
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Author Notes:Jose Arturo Gutierrez-Triana, Tinatini Tavhelidse, Thomas Thumberger, Isabelle Thomas, Beate Wittbrodt, Tanja Kellner, Kerim Anlas, Erika Tsingos and Joachim Wittbrodt

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