Is it possible to detect PEth 16: 0/18:1 and PEth 18:1/18:1 in red blood cells after 20 years of storage in liquid nitrogen?
IntroductionPhosphatidylethanol (PEth), as measured in freshly drawn blood samples, could be a promising new biomarker for habitual alcohol consumption, but it is still unknown whether PEth can also be determined from blood samples having been stored frozen for a longer period.Materials and methodsP...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
24 June 2017
|
| In: |
International journal of legal medicine
Year: 2017, Volume: 131, Issue: 5, Pages: 1291-1297 |
| ISSN: | 1437-1596 |
| DOI: | 10.1007/s00414-017-1626-2 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1007/s00414-017-1626-2 Verlag, Volltext: https://doi.org/10.1007/s00414-017-1626-2 
|
| Author Notes: | Robert Boll, Theron Johnson, Rudolf Kaaks, Tilman Kühn, Gisela Skopp |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1580760325 | ||
| 003 | DE-627 | ||
| 005 | 20250206100122.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180907s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00414-017-1626-2 |2 doi | |
| 035 | |a (DE-627)1580760325 | ||
| 035 | |a (DE-576)510760325 | ||
| 035 | |a (DE-599)BSZ510760325 | ||
| 035 | |a (OCoLC)1341018297 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Boll, Robert |e VerfasserIn |0 (DE-588)1166415082 |0 (DE-627)1030410860 |0 (DE-576)510759882 |4 aut | |
| 245 | 1 | 0 | |a Is it possible to detect PEth 16 |b 0/18:1 and PEth 18:1/18:1 in red blood cells after 20 years of storage in liquid nitrogen? |c Robert Boll, Theron Johnson, Rudolf Kaaks, Tilman Kühn, Gisela Skopp |
| 264 | 1 | |c 24 June 2017 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 07.09.2018 | ||
| 520 | |a IntroductionPhosphatidylethanol (PEth), as measured in freshly drawn blood samples, could be a promising new biomarker for habitual alcohol consumption, but it is still unknown whether PEth can also be determined from blood samples having been stored frozen for a longer period.Materials and methodsPEth 16:0/18:1 and PEth 18:1/18:1 were determined by LC-MS/MS from red blood cells (RBC) derived from blood samples of (I) 20 healthy volunteers (after 1 month of storage at −80 °C) and (II) 232 participants of the population-based European Prospective Investigation into Cancer and Nutrition (EPIC)-Heidelberg study (after 20 years of storage at −196 °C). Analyses involved liquid-liquid extraction from 100 μl aliquots with phosphatidylpropanol (PProp 18:1/18:1) as the internal standard. Extracts were subjected to a 10-min LC gradient separation, using multiple reaction monitoring of deprotonated molecules for quantification.ResultsAfter 1 month of storage at −80 °C, PEth was detectable in all samples at mean concentrations of 393.6 ± 12.4 ng/ml (PEth 16:0/18:1) and 43.3 ± 1.1 ng/ml (PEth 18:1/18:1). In samples stored for 20 years at −196 °C, PEth was detectable in 23.7% of all samples at mean concentrations of 412.2 ± 655.5 ng/ml (PEth 16:0/18:1) and 38.0 ± 74.8 ng/ml (PEth 18:1/18:1).Discussion and conclusionPEth can be determined reliably from samples of moderate habitual alcohol consumers after 1 month of storage at −80 °C. Our data suggest that PEth is generally also detectable in samples after 20 years of storage at −196 °C. Further studies are needed to assess the still unknown impact of storage duration and temperature on different PEth specimen concentrations. | ||
| 650 | 4 | |a EPIC-Heidelberg study | |
| 650 | 4 | |a Habitual alcohol consumption | |
| 650 | 4 | |a Long-term storage | |
| 650 | 4 | |a Phosphatidylethanol (PEth) | |
| 700 | 1 | |a Johnson, Theron S. |d 1972- |e VerfasserIn |0 (DE-588)13177526X |0 (DE-627)513947442 |0 (DE-576)298736012 |4 aut | |
| 700 | 1 | |a Kaaks, Rudolf |d 1960- |e VerfasserIn |0 (DE-588)172809223 |0 (DE-627)697739562 |0 (DE-576)133665518 |4 aut | |
| 700 | 1 | |a Kühn, Tilman |d 1979- |e VerfasserIn |0 (DE-588)1059123908 |0 (DE-627)798013915 |0 (DE-576)415142644 |4 aut | |
| 700 | 1 | |a Skopp, Gisela |d 1951- |e VerfasserIn |0 (DE-588)1060738538 |0 (DE-627)800969413 |0 (DE-576)164452346 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of legal medicine |d Berlin : Springer, 1990 |g 131(2017), 5, Seite 1291-1297 |h Online-Ressource |w (DE-627)253724295 |w (DE-600)1459222-8 |w (DE-576)072373148 |x 1437-1596 |7 nnas |a Is it possible to detect PEth 16 0/18:1 and PEth 18:1/18:1 in red blood cells after 20 years of storage in liquid nitrogen? |
| 773 | 1 | 8 | |g volume:131 |g year:2017 |g number:5 |g pages:1291-1297 |g extent:7 |a Is it possible to detect PEth 16 0/18:1 and PEth 18:1/18:1 in red blood cells after 20 years of storage in liquid nitrogen? |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00414-017-1626-2 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00414-017-1626-2 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180907 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 1060738538 |a Skopp, Gisela |m 1060738538:Skopp, Gisela |d 910000 |d 912100 |e 910000PS1060738538 |e 912100PS1060738538 |k 0/910000/ |k 1/910000/912100/ |p 5 |y j | ||
| 998 | |g 1059123908 |a Kühn, Tilman |m 1059123908:Kühn, Tilman |d 50000 |e 50000PK1059123908 |k 0/50000/ |p 4 | ||
| 998 | |g 172809223 |a Kaaks, Rudolf |m 172809223:Kaaks, Rudolf |d 50000 |e 50000PK172809223 |k 0/50000/ |p 3 | ||
| 998 | |g 13177526X |a Johnson, Theron S. |m 13177526X:Johnson, Theron S. |d 910000 |d 911300 |e 910000PJ13177526X |e 911300PJ13177526X |k 0/910000/ |k 1/910000/911300/ |p 2 | ||
| 999 | |a KXP-PPN1580760325 |e 3024873244 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1580760325","note":["Gesehen am 07.09.2018"],"name":{"displayForm":["Robert Boll, Theron Johnson, Rudolf Kaaks, Tilman Kühn, Gisela Skopp"]},"relHost":[{"disp":"Is it possible to detect PEth 16 0/18:1 and PEth 18:1/18:1 in red blood cells after 20 years of storage in liquid nitrogen?International journal of legal medicine","pubHistory":["Volume 104, issue 1 (January 1990)-"],"note":["Gesehen am 06.03.25","Volltext auch als Teil einer Datenbank verfügbar"],"recId":"253724295","type":{"media":"Online-Ressource","bibl":"periodical"},"id":{"eki":["253724295"],"issn":["1437-1596"],"zdb":["1459222-8"]},"language":["eng","ger"],"origin":[{"dateIssuedKey":"1990","publisher":"Springer ; HeinOnline ; Springer","publisherPlace":"Berlin ; Heidelberg ; Getzville, NY ; Berlin ; Heidelberg ; New York, NY","dateIssuedDisp":"1990-"}],"titleAlt":[{"title":"Legal medicine"}],"title":[{"title_sort":"International journal of legal medicine","subtitle":"official publication of the International Academy of Legal Medicine","title":"International journal of legal medicine"}],"physDesc":[{"extent":"Online-Ressource"}],"part":{"text":"131(2017), 5, Seite 1291-1297","pages":"1291-1297","volume":"131","issue":"5","extent":"7","year":"2017"}}],"person":[{"display":"Boll, Robert","family":"Boll","given":"Robert","role":"aut"},{"given":"Theron S.","display":"Johnson, Theron S.","family":"Johnson","role":"aut"},{"family":"Kaaks","display":"Kaaks, Rudolf","given":"Rudolf","role":"aut"},{"role":"aut","family":"Kühn","display":"Kühn, Tilman","given":"Tilman"},{"given":"Gisela","display":"Skopp, Gisela","family":"Skopp","role":"aut"}],"title":[{"title_sort":"Is it possible to detect PEth 16","subtitle":"0/18:1 and PEth 18:1/18:1 in red blood cells after 20 years of storage in liquid nitrogen?","title":"Is it possible to detect PEth 16"}],"physDesc":[{"extent":"7 S."}],"origin":[{"dateIssuedKey":"2017","dateIssuedDisp":"24 June 2017"}],"id":{"doi":["10.1007/s00414-017-1626-2"],"eki":["1580760325"]},"language":["eng"],"type":{"media":"Online-Ressource","bibl":"article-journal"}} | ||
| SRT | |a BOLLROBERTISITPOSSIB2420 | ||