Time-lapse imaging of neuroblastoma cells to determine cell fate upon gene knockdown
Neuroblastoma is the most common extra-cranial solid tumor of early childhood. Standard therapies are not effective in case of poor prognosis and chemotherapy resistance. To improve drug therapy, it is imperative to discover new targets that play a substantial role in tumorigenesis of neuroblastoma....
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
December 12, 2012
|
| In: |
PLOS ONE
Year: 2012, Jahrgang: 7, Heft: 12) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0050988 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1371/journal.pone.0050988 Verlag, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0050988 
|
| Verfasserangaben: | Richa Batra, Nathalie Harder, Sina Gogolin, Nicolle Diessl, Zita Soons, Christina Jäger-Schmidt, Christian Lawerenz, Roland Eils, Karl Rohr, Frank Westermann, Rainer König |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1580888208 | ||
| 003 | DE-627 | ||
| 005 | 20220815005721.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180912s2012 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.pone.0050988 |2 doi | |
| 035 | |a (DE-627)1580888208 | ||
| 035 | |a (DE-576)510888208 | ||
| 035 | |a (DE-599)BSZ510888208 | ||
| 035 | |a (OCoLC)1341018529 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Batra, Richa |e VerfasserIn |0 (DE-588)1035418657 |0 (DE-627)747808090 |0 (DE-576)382997166 |4 aut | |
| 245 | 1 | 0 | |a Time-lapse imaging of neuroblastoma cells to determine cell fate upon gene knockdown |c Richa Batra, Nathalie Harder, Sina Gogolin, Nicolle Diessl, Zita Soons, Christina Jäger-Schmidt, Christian Lawerenz, Roland Eils, Karl Rohr, Frank Westermann, Rainer König |
| 264 | 1 | |c December 12, 2012 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 12.09.2018 | ||
| 520 | |a Neuroblastoma is the most common extra-cranial solid tumor of early childhood. Standard therapies are not effective in case of poor prognosis and chemotherapy resistance. To improve drug therapy, it is imperative to discover new targets that play a substantial role in tumorigenesis of neuroblastoma. The mitotic machinery is an attractive target for therapeutic interventions and inhibitors can be developed to target mitotic entry, spindle apparatus, spindle activation checkpoint, and mitotic exit. We present an elaborate analysis pipeline to determine cancer specific therapeutic targets by first performing a focused gene expression analysis to select genes followed by a gene knockdown screening assay of live cells. We interrogated gene expression studies of neuroblastoma tumors and selected 240 genes relevant for tumorigenesis and cell cycle. With these genes we performed time-lapse screening of gene knockdowns in neuroblastoma cells. We classified cellular phenotypes and used the temporal context of the perturbation effect to determine the sequence of events, particularly the mitotic entry preceding cell death. Based upon this phenotype kinetics from the gene knockdown screening, we inferred dynamic gene functions in mitosis and cell proliferation. We identified six genes (DLGAP5, DSCC1, SMO, SNRPD1, SSBP1, and UBE2C) with a vital role in mitosis and these are promising therapeutic targets for neuroblastoma. Images and movies of every time point of all screened genes are available at https://ichip.bioquant.uni-heidelberg.de. | ||
| 650 | 4 | |a Cell cycle and cell division | |
| 650 | 4 | |a Cell death | |
| 650 | 4 | |a Gene expression | |
| 650 | 4 | |a Genetic screens | |
| 650 | 4 | |a Mitosis | |
| 650 | 4 | |a Neuroblastoma | |
| 650 | 4 | |a Neuroblastoma cells | |
| 650 | 4 | |a Small interfering RNAs | |
| 700 | 1 | |a Harder, Nathalie |e VerfasserIn |0 (DE-588)1073949370 |0 (DE-627)830053417 |0 (DE-576)327502266 |4 aut | |
| 700 | 1 | |a Soons, Zita |e VerfasserIn |0 (DE-588)1162716339 |0 (DE-627)1026889766 |0 (DE-576)507592778 |4 aut | |
| 700 | 1 | |a Jäger-Schmidt, Christina |e VerfasserIn |0 (DE-588)1227603142 |0 (DE-627)1748594028 |4 aut | |
| 700 | 1 | |a Lawerenz, Christian |e VerfasserIn |0 (DE-588)1182053327 |0 (DE-627)1662547374 |4 aut | |
| 700 | 1 | |a Eils, Roland |d 1965- |e VerfasserIn |0 (DE-588)1020648287 |0 (DE-627)691291705 |0 (DE-576)361718195 |4 aut | |
| 700 | 1 | |a Rohr, Karl |e VerfasserIn |0 (DE-588)137474466 |0 (DE-627)695829440 |0 (DE-576)303788593 |4 aut | |
| 700 | 1 | |a Westermann, Frank |d 1968- |e VerfasserIn |0 (DE-588)118119990 |0 (DE-627)69468628X |0 (DE-576)291725570 |4 aut | |
| 700 | 1 | |a König, Rainer |d 1968- |e VerfasserIn |0 (DE-588)121507076 |0 (DE-627)705500535 |0 (DE-576)292745451 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PLOS ONE |d San Francisco, California, US : PLOS, 2006 |g 7(2012,12), Artikel-Nummer e50988, 10 Seiten |h Online-Ressource |w (DE-627)523574592 |w (DE-600)2267670-3 |w (DE-576)281331979 |x 1932-6203 |7 nnas |a Time-lapse imaging of neuroblastoma cells to determine cell fate upon gene knockdown |
| 773 | 1 | 8 | |g volume:7 |g year:2012 |g number:12) |g extent:10 |a Time-lapse imaging of neuroblastoma cells to determine cell fate upon gene knockdown |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1371/journal.pone.0050988 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0050988 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180912 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 121507076 |a König, Rainer |m 121507076:König, Rainer |d 160000 |d 160100 |d 700000 |d 716000 |e 160000PK121507076 |e 160100PK121507076 |e 700000PK121507076 |e 716000PK121507076 |k 0/160000/ |k 1/160000/160100/ |k 0/700000/ |k 1/700000/716000/ |p 11 |y j | ||
| 998 | |g 118119990 |a Westermann, Frank |m 118119990:Westermann, Frank |d 50000 |e 50000PW118119990 |k 0/50000/ |p 10 | ||
| 998 | |g 137474466 |a Rohr, Karl |m 137474466:Rohr, Karl |d 160000 |d 160100 |d 700000 |d 716000 |d 700000 |d 718000 |e 160000PR137474466 |e 160100PR137474466 |e 700000PR137474466 |e 716000PR137474466 |e 700000PR137474466 |e 718000PR137474466 |k 0/160000/ |k 1/160000/160100/ |k 0/700000/ |k 1/700000/716000/ |k 0/700000/ |k 1/700000/718000/ |p 9 | ||
| 998 | |g 1020648287 |a Eils, Roland |m 1020648287:Eils, Roland |d 160000 |d 160100 |d 700000 |d 716000 |d 700000 |d 718000 |e 160000PE1020648287 |e 160100PE1020648287 |e 700000PE1020648287 |e 716000PE1020648287 |e 700000PE1020648287 |e 718000PE1020648287 |k 0/160000/ |k 1/160000/160100/ |k 0/700000/ |k 1/700000/716000/ |k 0/700000/ |k 1/700000/718000/ |p 8 | ||
| 998 | |g 1182053327 |a Lawerenz, Christian |m 1182053327:Lawerenz, Christian |d 50000 |e 50000PL1182053327 |k 0/50000/ |p 7 | ||
| 998 | |g 1227603142 |a Jäger-Schmidt, Christina |m 1227603142:Jäger-Schmidt, Christina |d 50000 |e 50000PJ1227603142 |k 0/50000/ |p 6 | ||
| 998 | |g 1162716339 |a Soons, Zita |m 1162716339:Soons, Zita |d 160000 |d 160100 |d 700000 |d 716000 |e 160000PS1162716339 |e 160100PS1162716339 |e 700000PS1162716339 |e 716000PS1162716339 |k 0/160000/ |k 1/160000/160100/ |k 0/700000/ |k 1/700000/716000/ |p 5 | ||
| 998 | |g 1073949370 |a Harder, Nathalie |m 1073949370:Harder, Nathalie |d 160000 |d 160100 |d 700000 |d 716000 |e 160000PH1073949370 |e 160100PH1073949370 |e 700000PH1073949370 |e 716000PH1073949370 |k 0/160000/ |k 1/160000/160100/ |k 0/700000/ |k 1/700000/716000/ |p 2 | ||
| 998 | |g 1035418657 |a Batra, Richa |m 1035418657:Batra, Richa |d 140000 |d 700000 |d 716000 |e 140000PB1035418657 |e 700000PB1035418657 |e 716000PB1035418657 |k 0/140000/ |k 0/700000/ |k 1/700000/716000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1580888208 |e 3025321075 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 12.09.2018"],"relHost":[{"corporate":[{"display":"Public Library of Science","role":"isb"}],"origin":[{"dateIssuedDisp":"2006-","publisher":"PLOS ; PLoS","publisherPlace":"San Francisco, California, US ; Lawrence, Kan.","dateIssuedKey":"2006"}],"name":{"displayForm":["Public Library of Science"]},"title":[{"title_sort":"PLOS ONE","title":"PLOS ONE"}],"physDesc":[{"extent":"Online-Ressource"}],"part":{"volume":"7","text":"7(2012,12), Artikel-Nummer e50988, 10 Seiten","issue":"12)","year":"2012","extent":"10"},"id":{"zdb":["2267670-3"],"issn":["1932-6203"],"eki":["523574592"]},"note":["Schreibweise des Titels bis 2012: PLoS ONE","Gesehen am 20.03.19"],"recId":"523574592","disp":"Time-lapse imaging of neuroblastoma cells to determine cell fate upon gene knockdownPLOS ONE","language":["eng"],"type":{"media":"Online-Ressource","bibl":"periodical"},"pubHistory":["1.2006 -"]}],"id":{"doi":["10.1371/journal.pone.0050988"],"eki":["1580888208"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"physDesc":[{"extent":"10 S."}],"title":[{"title":"Time-lapse imaging of neuroblastoma cells to determine cell fate upon gene knockdown","title_sort":"Time-lapse imaging of neuroblastoma cells to determine cell fate upon gene knockdown"}],"language":["eng"],"name":{"displayForm":["Richa Batra, Nathalie Harder, Sina Gogolin, Nicolle Diessl, Zita Soons, Christina Jäger-Schmidt, Christian Lawerenz, Roland Eils, Karl Rohr, Frank Westermann, Rainer König"]},"origin":[{"dateIssuedDisp":"December 12, 2012","dateIssuedKey":"2012"}],"recId":"1580888208","person":[{"role":"aut","family":"Batra","given":"Richa","display":"Batra, Richa"},{"role":"aut","given":"Nathalie","display":"Harder, Nathalie","family":"Harder"},{"role":"aut","display":"Soons, Zita","given":"Zita","family":"Soons"},{"role":"aut","family":"Jäger-Schmidt","display":"Jäger-Schmidt, Christina","given":"Christina"},{"role":"aut","display":"Lawerenz, Christian","given":"Christian","family":"Lawerenz"},{"role":"aut","family":"Eils","given":"Roland","display":"Eils, Roland"},{"role":"aut","family":"Rohr","display":"Rohr, Karl","given":"Karl"},{"given":"Frank","display":"Westermann, Frank","family":"Westermann","role":"aut"},{"display":"König, Rainer","given":"Rainer","family":"König","role":"aut"}]} | ||
| SRT | |a BATRARICHATIMELAPSEI1220 | ||