Isolation of peroxisomes from rat liver and cultured hepatoma cells by density gradient centrifugation
Subcellular fractionation is still a valuable technique to unravel organelle-specific proteomes, validate the location of uncharacterized proteins, or to functionally analyze import and metabolism in individual subcellular compartments. In this respect, density gradient centrifugation still represen...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Kapitel/Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
14 April 2017
|
| In: |
Peroxisomes
Year: 2017, Pages: 1-11 |
| DOI: | 10.1007/978-1-4939-6937-1_1 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1007/978-1-4939-6937-1_1
|
| Verfasserangaben: | Andreas Manner, Markus Islinger |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1581029578 | ||
| 003 | DE-627 | ||
| 005 | 20220815012136.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180917s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/978-1-4939-6937-1_1 |2 doi | |
| 035 | |a (DE-627)1581029578 | ||
| 035 | |a (DE-576)511029578 | ||
| 035 | |a (DE-599)BSZ511029578 | ||
| 035 | |a (OCoLC)1341018773 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Manner, Andreas |d 1989- |e VerfasserIn |0 (DE-588)1167193261 |0 (DE-627)1030881863 |0 (DE-576)511029349 |4 aut | |
| 245 | 1 | 0 | |a Isolation of peroxisomes from rat liver and cultured hepatoma cells by density gradient centrifugation |c Andreas Manner, Markus Islinger |
| 264 | 1 | |c 14 April 2017 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 17.09.2018 | ||
| 520 | |a Subcellular fractionation is still a valuable technique to unravel organelle-specific proteomes, validate the location of uncharacterized proteins, or to functionally analyze import and metabolism in individual subcellular compartments. In this respect, density gradient centrifugation still represents a very classic, indispensable technique to isolate and analyze peroxisomes. Here, we present two independent protocols for the purification of peroxisomes from either liver tissue or the HepG2 hepatoma cell line. While the former permits the isolation of highly pure peroxisomes suitable for, e.g., subcellular proteomics experiments, the latter protocol yields peroxisomal fractions from considerably less purity but allows to easily modify metabolic conditions in the culture medium or to genetically manipulate the peroxisomal compartment. In this respect, both purification methods represent alternative tools to be applied in experiments investigating peroxisome physiology. | ||
| 650 | 4 | |a ACAD11 | |
| 650 | 4 | |a Density gradient centrifugation | |
| 650 | 4 | |a Liver | |
| 650 | 4 | |a Organelle purification | |
| 650 | 4 | |a Peroxisomes | |
| 700 | 1 | |a Islinger, Markus |d 1968- |e VerfasserIn |0 (DE-588)123008166 |0 (DE-627)706126262 |0 (DE-576)293518904 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Peroxisomes |d New York, NY : Humana Press, 2017 |g (2017), Seite 1-11 |h Online-Ressource (XVII, 347 p. 63 illus., 36 illus. in color, online resource) |w (DE-627)1658175719 |w (DE-576)48887064X |z 9781493969371 |7 nnam |a Isolation of peroxisomes from rat liver and cultured hepatoma cells by density gradient centrifugation |
| 773 | 1 | 8 | |g year:2017 |g pages:1-11 |g extent:11 |a Isolation of peroxisomes from rat liver and cultured hepatoma cells by density gradient centrifugation |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-4939-6937-1_1 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180917 | ||
| 993 | |a BookComponentPart | ||
| 994 | |a 2017 | ||
| 998 | |g 123008166 |a Islinger, Markus |m 123008166:Islinger, Markus |d 60000 |d 999810 |d 50000 |e 60000PI123008166 |e 999810PI123008166 |e 50000PI123008166 |k 0/60000/ |k 1/60000/999810/ |k 0/50000/ |p 2 |y j | ||
| 998 | |g 1167193261 |a Manner, Andreas |m 1167193261:Manner, Andreas |d 60000 |d 999810 |e 60000PM1167193261 |e 999810PM1167193261 |k 0/60000/ |k 1/60000/999810/ |p 1 |x j | ||
| 999 | |a KXP-PPN1581029578 |e 3025712414 | ||
| BIB | |a Y | ||
| JSO | |a {"name":{"displayForm":["Andreas Manner, Markus Islinger"]},"id":{"eki":["1581029578"],"doi":["10.1007/978-1-4939-6937-1_1"]},"origin":[{"dateIssuedKey":"2017","dateIssuedDisp":"14 April 2017"}],"relHost":[{"origin":[{"dateIssuedDisp":"2017","publisher":"Humana Press","dateIssuedKey":"2017","publisherPlace":"New York, NY"}],"id":{"isbn":["9781493969371"],"doi":["10.1007/978-1-4939-6937-1"],"eki":["1658175719"]},"name":{"displayForm":["edited by Michael Schrader"]},"relMultPart":[{"id":{"eki":["599671882"],"zdb":["2493551-7"],"issn":["1940-6029"]},"origin":[{"publisher":"Humana Press ; [Springer]","dateIssuedKey":"1984","dateIssuedDisp":"1984-","publisherPlace":"Totowa, NJ ; [Heidelberg]"}],"dispAlt":"Methods in molecular biology","physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Methods in molecular biology","title":"Methods in molecular biology"}],"part":{"number":["1595"],"number_sort":["1595"]},"pubHistory":["1.1984 -"],"language":["eng"],"recId":"599671882","note":["Gesehen am 05.11.12"],"type":{"media":"Online-Ressource","bibl":"serial"},"disp":"Methods in Molecular Biology"}],"physDesc":[{"extent":"Online-Ressource (XVII, 347 p. 63 illus., 36 illus. in color, online resource)"}],"title":[{"title_sort":"Peroxisomes","title":"Peroxisomes","subtitle":"Methods and Protocols"}],"person":[{"roleDisplay":"Hrsg.","display":"Schrader, Michael","role":"edt","family":"Schrader","given":"Michael"}],"part":{"extent":"11","text":"(2017), Seite 1-11","pages":"1-11","year":"2017"},"type":{"bibl":"edited-book","media":"Online-Ressource"},"disp":"Isolation of peroxisomes from rat liver and cultured hepatoma cells by density gradient centrifugationPeroxisomes","language":["eng"],"recId":"1658175719"}],"physDesc":[{"extent":"11 S."}],"person":[{"role":"aut","roleDisplay":"VerfasserIn","display":"Manner, Andreas","given":"Andreas","family":"Manner"},{"family":"Islinger","given":"Markus","display":"Islinger, Markus","roleDisplay":"VerfasserIn","role":"aut"}],"title":[{"title":"Isolation of peroxisomes from rat liver and cultured hepatoma cells by density gradient centrifugation","title_sort":"Isolation of peroxisomes from rat liver and cultured hepatoma cells by density gradient centrifugation"}],"recId":"1581029578","language":["eng"],"type":{"bibl":"chapter","media":"Online-Ressource"},"note":["Gesehen am 17.09.2018"]} | ||
| SRT | |a MANNERANDRISOLATIONO1420 | ||