In vivo protein crystallization opens new routes in structural biology
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of fre...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2012 Jan 29
|
| In: |
Nature methods
Year: 2012, Jahrgang: 9, Heft: 3, Pages: 259-262 |
| ISSN: | 1548-7105 |
| DOI: | 10.1038/nmeth.1859 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1038/nmeth.1859 Verlag, Volltext: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429599/ 
|
| Verfasserangaben: | Rudolf Koopmann, Karolina Cupelli, Lars Redecke, Karol Nass, Daniel P DePonte, Thomas A White, Francesco Stellato, Dirk Rehders, Mengning Liang, Jakob Andreasson, Andrew Aquila, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J Bogan, Christoph Bostedt, Sébastien Boutet, John D Bozek, Carl Caleman, Nicola Coppola, Jan Davidsson, R Bruce Doak, Tomas Ekeberg, Sascha W Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y Hampton, Andreas Hartmann, Robert Hartmann, Günter Hauser, Helmut Hirsemann, Peter Holl, Mark S Hunter, Stephan Kassemeyer, Richard A Kirian, Lukas Lomb, Filipe RNC Maia, Nils Kimmel, Andrew V Martin, Marc Messerschmidt, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M Marvin Seibert, Robert L Shoeman, Raymond G Sierra, Heike Soltau, Stephan Stern, Lothar Strüder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Georg Weidenspointner, Uwe Weierstall, Garth J Williams, Cornelia B Wunderer, Petra Fromme, John CH Spence, Thilo Stehle, Henry N Chapman, Christian Betzel, and Michael Duszenko |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1581066252 | ||
| 003 | DE-627 | ||
| 005 | 20220815012849.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180918s2012 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/nmeth.1859 |2 doi | |
| 035 | |a (DE-627)1581066252 | ||
| 035 | |a (DE-576)511066252 | ||
| 035 | |a (DE-599)BSZ511066252 | ||
| 035 | |a (OCoLC)1341018892 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 29 |2 sdnb | ||
| 100 | 1 | |a Koopmann, Rudolf |d 1978- |e VerfasserIn |0 (DE-588)141971711 |0 (DE-627)632851465 |0 (DE-576)327137789 |4 aut | |
| 245 | 1 | 0 | |a In vivo protein crystallization opens new routes in structural biology |c Rudolf Koopmann, Karolina Cupelli, Lars Redecke, Karol Nass, Daniel P DePonte, Thomas A White, Francesco Stellato, Dirk Rehders, Mengning Liang, Jakob Andreasson, Andrew Aquila, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J Bogan, Christoph Bostedt, Sébastien Boutet, John D Bozek, Carl Caleman, Nicola Coppola, Jan Davidsson, R Bruce Doak, Tomas Ekeberg, Sascha W Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y Hampton, Andreas Hartmann, Robert Hartmann, Günter Hauser, Helmut Hirsemann, Peter Holl, Mark S Hunter, Stephan Kassemeyer, Richard A Kirian, Lukas Lomb, Filipe RNC Maia, Nils Kimmel, Andrew V Martin, Marc Messerschmidt, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M Marvin Seibert, Robert L Shoeman, Raymond G Sierra, Heike Soltau, Stephan Stern, Lothar Strüder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Georg Weidenspointner, Uwe Weierstall, Garth J Williams, Cornelia B Wunderer, Petra Fromme, John CH Spence, Thilo Stehle, Henry N Chapman, Christian Betzel, and Michael Duszenko |
| 246 | 3 | 0 | |a Im Titel ist in vivo kursiv geschrieben |
| 264 | 1 | |c 2012 Jan 29 | |
| 300 | |a 4 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 18.09.2018 | ||
| 520 | |a Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology. | ||
| 700 | 1 | |a Epp, Sascha W. |e VerfasserIn |0 (DE-588)133262448 |0 (DE-627)540508411 |0 (DE-576)29973367X |4 aut | |
| 700 | 1 | |a Erk, Benjamin Michael Dieter |e VerfasserIn |0 (DE-588)1030558949 |0 (DE-627)735490392 |0 (DE-576)37841853X |4 aut | |
| 700 | 1 | |a Foucar, Lutz Michael |d 1977- |e VerfasserIn |0 (DE-588)136389910 |0 (DE-627)582070864 |0 (DE-576)300995687 |4 aut | |
| 700 | 1 | |a Kassemeyer, Stephan |e VerfasserIn |0 (DE-588)1057879665 |0 (DE-627)795360495 |0 (DE-576)413885445 |4 aut | |
| 700 | 1 | |a Lomb, Lukas |e VerfasserIn |0 (DE-588)1024381234 |0 (DE-627)719798418 |0 (DE-576)368388794 |4 aut | |
| 700 | 1 | |a Rolles, Daniel |d 1976- |e VerfasserIn |0 (DE-588)130065242 |0 (DE-627)489612725 |0 (DE-576)297978020 |4 aut | |
| 700 | 1 | |a Rudek, Benedikt |e VerfasserIn |0 (DE-588)1023007266 |0 (DE-627)717344789 |0 (DE-576)366259563 |4 aut | |
| 700 | 1 | |a Rudenko, Artem |e VerfasserIn |0 (DE-588)1160486670 |0 (DE-627)1023720671 |0 (DE-576)505941341 |4 aut | |
| 700 | 1 | |a Schlichting, Ilme |d 1960- |e VerfasserIn |0 (DE-588)112690262 |0 (DE-627)69115743X |0 (DE-576)302808477 |4 aut | |
| 700 | 1 | |a Shoeman, Robert L. |e VerfasserIn |0 (DE-588)1160492727 |0 (DE-627)1023728796 |0 (DE-576)505947587 |4 aut | |
| 700 | 1 | |a Ullrich, Joachim |d 1956- |e VerfasserIn |0 (DE-588)142919640 |0 (DE-627)704342928 |0 (DE-576)334086973 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nature methods |d London [u.a.] : Nature Publishing Group, 2004 |g 9(2012), 3, Seite 259-262 |h Online-Ressource |w (DE-627)397615310 |w (DE-600)2163081-1 |w (DE-576)118489089 |x 1548-7105 |7 nnas |a In vivo protein crystallization opens new routes in structural biology |
| 773 | 1 | 8 | |g volume:9 |g year:2012 |g number:3 |g pages:259-262 |g extent:4 |a In vivo protein crystallization opens new routes in structural biology |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1038/nmeth.1859 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429599/ |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180918 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 142919640 |a Ullrich, Joachim |m 142919640:Ullrich, Joachim |d 130000 |e 130000PU142919640 |k 0/130000/ |p 58 | ||
| 998 | |g 1160492727 |a Shoeman, Robert L. |m 1160492727:Shoeman, Robert L. |p 52 | ||
| 998 | |g 112690262 |a Schlichting, Ilme |m 112690262:Schlichting, Ilme |d 120000 |d 120300 |d 700000 |d 718000 |e 120000PS112690262 |e 120300PS112690262 |e 700000PS112690262 |e 718000PS112690262 |k 0/120000/ |k 1/120000/120300/ |k 0/700000/ |k 1/700000/718000/ |p 49 | ||
| 998 | |g 1160486670 |a Rudenko, Artem |m 1160486670:Rudenko, Artem |p 48 | ||
| 998 | |g 1023007266 |a Rudek, Benedikt |m 1023007266:Rudek, Benedikt |p 47 | ||
| 998 | |g 130065242 |a Rolles, Daniel |m 130065242:Rolles, Daniel |p 46 | ||
| 998 | |g 1024381234 |a Lomb, Lukas |m 1024381234:Lomb, Lukas |p 40 | ||
| 998 | |g 1057879665 |a Kassemeyer, Stephan |m 1057879665:Kassemeyer, Stephan |p 38 | ||
| 998 | |g 136389910 |a Foucar, Lutz |m 136389910:Foucar, Lutz |p 27 | ||
| 998 | |g 1030558949 |a Erk, Benjamin Michael Dieter |m 1030558949:Erk, Benjamin Michael Dieter |p 25 | ||
| 998 | |g 133262448 |a Epp, Sascha W. |m 133262448:Epp, Sascha W. |p 24 | ||
| 999 | |a KXP-PPN1581066252 |e 3025777834 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"roleDisplay":"VerfasserIn","display":"Koopmann, Rudolf","role":"aut","family":"Koopmann","given":"Rudolf"},{"roleDisplay":"VerfasserIn","display":"Epp, Sascha W.","role":"aut","family":"Epp","given":"Sascha W."},{"roleDisplay":"VerfasserIn","display":"Erk, Benjamin Michael Dieter","role":"aut","family":"Erk","given":"Benjamin Michael Dieter"},{"family":"Foucar","given":"Lutz Michael","roleDisplay":"VerfasserIn","display":"Foucar, Lutz Michael","role":"aut"},{"display":"Kassemeyer, Stephan","roleDisplay":"VerfasserIn","role":"aut","family":"Kassemeyer","given":"Stephan"},{"role":"aut","display":"Lomb, Lukas","roleDisplay":"VerfasserIn","given":"Lukas","family":"Lomb"},{"given":"Daniel","family":"Rolles","role":"aut","display":"Rolles, Daniel","roleDisplay":"VerfasserIn"},{"family":"Rudek","given":"Benedikt","display":"Rudek, Benedikt","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Rudenko","given":"Artem","roleDisplay":"VerfasserIn","display":"Rudenko, Artem","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Schlichting, Ilme","role":"aut","family":"Schlichting","given":"Ilme"},{"display":"Shoeman, Robert L.","roleDisplay":"VerfasserIn","role":"aut","family":"Shoeman","given":"Robert L."},{"given":"Joachim","family":"Ullrich","role":"aut","roleDisplay":"VerfasserIn","display":"Ullrich, Joachim"}],"title":[{"title":"In vivo protein crystallization opens new routes in structural biology","title_sort":"In vivo protein crystallization opens new routes in structural biology"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 18.09.2018"],"recId":"1581066252","language":["eng"],"name":{"displayForm":["Rudolf Koopmann, Karolina Cupelli, Lars Redecke, Karol Nass, Daniel P DePonte, Thomas A White, Francesco Stellato, Dirk Rehders, Mengning Liang, Jakob Andreasson, Andrew Aquila, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J Bogan, Christoph Bostedt, Sébastien Boutet, John D Bozek, Carl Caleman, Nicola Coppola, Jan Davidsson, R Bruce Doak, Tomas Ekeberg, Sascha W Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y Hampton, Andreas Hartmann, Robert Hartmann, Günter Hauser, Helmut Hirsemann, Peter Holl, Mark S Hunter, Stephan Kassemeyer, Richard A Kirian, Lukas Lomb, Filipe RNC Maia, Nils Kimmel, Andrew V Martin, Marc Messerschmidt, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M Marvin Seibert, Robert L Shoeman, Raymond G Sierra, Heike Soltau, Stephan Stern, Lothar Strüder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Georg Weidenspointner, Uwe Weierstall, Garth J Williams, Cornelia B Wunderer, Petra Fromme, John CH Spence, Thilo Stehle, Henry N Chapman, Christian Betzel, and Michael Duszenko"]},"origin":[{"dateIssuedDisp":"2012 Jan 29","dateIssuedKey":"2012"}],"id":{"eki":["1581066252"],"doi":["10.1038/nmeth.1859"]},"physDesc":[{"extent":"4 S."}],"relHost":[{"pubHistory":["1.2004 -"],"part":{"year":"2012","pages":"259-262","issue":"3","volume":"9","text":"9(2012), 3, Seite 259-262","extent":"4"},"note":["Gesehen am 14. August 2018"],"type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"In vivo protein crystallization opens new routes in structural biologyNature methods","language":["eng"],"recId":"397615310","title":[{"title_sort":"Nature methods","title":"Nature methods","subtitle":"techniques for life scientists and chemists"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"dateIssuedDisp":"2004-","publisher":"Nature Publishing Group","dateIssuedKey":"2004","publisherPlace":"London [u.a.]"}],"id":{"issn":["1548-7105"],"zdb":["2163081-1"],"eki":["397615310"]}}]} | ||
| SRT | |a KOOPMANNRUINVIVOPROT2012 | ||