FoxO3a suppression and VPS34 activity are essential to anti-atrophic effects of leucine in skeletal muscle
Our aim is to gain insight into the mechanisms underlying the anti-atrophic effects of leucine, namely, the way that this amino acid can restrain the up-regulation of MuRF1 and Mafbx/Atrogin-1 in muscle atrophy. Male rats received dietary leucine supplementation for 1-3 days, during which time their...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
24 April 2017
|
| In: |
Cell & tissue research
Year: 2017, Jahrgang: 369, Heft: 2, Pages: 381-394 |
| ISSN: | 1432-0878 |
| DOI: | 10.1007/s00441-017-2614-z |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1007/s00441-017-2614-z Verlag, Volltext: https://doi.org/10.1007/s00441-017-2614-z 
|
| Verfasserangaben: | Igor L. Baptista, João G. Silvestre, William J. Silva, Siegfried Labeit, Anselmo S. Moriscot |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1581095724 | ||
| 003 | DE-627 | ||
| 005 | 20220815013347.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180919s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00441-017-2614-z |2 doi | |
| 035 | |a (DE-627)1581095724 | ||
| 035 | |a (DE-576)511095724 | ||
| 035 | |a (DE-599)BSZ511095724 | ||
| 035 | |a (OCoLC)1341018911 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Baptista, Igor L. |e VerfasserIn |0 (DE-588)1167336410 |0 (DE-627)1031003851 |0 (DE-576)511110324 |4 aut | |
| 245 | 1 | 0 | |a FoxO3a suppression and VPS34 activity are essential to anti-atrophic effects of leucine in skeletal muscle |c Igor L. Baptista, João G. Silvestre, William J. Silva, Siegfried Labeit, Anselmo S. Moriscot |
| 246 | 3 | 3 | |a FoxO 3 a suppression and VPS 34 activity are essential to anti-atrophic effects of leucine in skeletal muscle |
| 264 | 1 | |c 24 April 2017 | |
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Im Titel sind die Ziffern „3“ und "34" tiefgestellt | ||
| 500 | |a Gesehen am 19.09.2018 | ||
| 520 | |a Our aim is to gain insight into the mechanisms underlying the anti-atrophic effects of leucine, namely, the way that this amino acid can restrain the up-regulation of MuRF1 and Mafbx/Atrogin-1 in muscle atrophy. Male rats received dietary leucine supplementation for 1-3 days, during which time their hind limbs were immobilized. Our results showed that leucine inhibited Forkhead Box O3 (FoxO3a) translocation to cell nuclei. In addition, leucine was able to reverse the expected reduction of FoXO3a ubiquitination caused by immobilization. Unexpectedly, leucine promoted these effects independently of the Class I PI3K/Akt pathway. Vacuolar protein sorting 34 (VPS34; a Class III PI3K) was strongly localized in nuclei after immobilization and leucine supplementation was able to prevent this effect. In experiments on cultured primary myotubes, dexamethasone led to the localization of VPS34 in the nucleus. In addition, the pharmacological inhibition of VPS34 blocked VPS34 nuclear localization and impaired the protective effect of leucine upon myotube trophicity. Finally, the pharmacological inhibition of VPS34 in primary myotubes prevented the protective effects of leucine upon MuRF1 and Mafbx/Atrogin-1 gene expression. Autophagy-related target genes were not responsive to leucine. Thus, we demonstrate that the anti-atrophic effect of leucine is dependent upon FoxO3a suppression and VPS34 activity. | ||
| 650 | 4 | |a Atrogene expression | |
| 650 | 4 | |a Atrophy | |
| 650 | 4 | |a Class III PI3K | |
| 650 | 4 | |a FoxO3a | |
| 650 | 4 | |a Skeletal muscle | |
| 700 | 1 | |a Labeit, Siegfried |d 1960- |e VerfasserIn |0 (DE-588)1033566837 |0 (DE-627)741562375 |0 (DE-576)381187314 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cell & tissue research |d Berlin : Springer, 1974 |g 369(2017), 2, Seite 381-394 |h Online-Ressource |w (DE-627)253390516 |w (DE-600)1458496-7 |w (DE-576)07228353X |x 1432-0878 |7 nnas |a FoxO3a suppression and VPS34 activity are essential to anti-atrophic effects of leucine in skeletal muscle |
| 773 | 1 | 8 | |g volume:369 |g year:2017 |g number:2 |g pages:381-394 |g extent:14 |a FoxO3a suppression and VPS34 activity are essential to anti-atrophic effects of leucine in skeletal muscle |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00441-017-2614-z |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00441-017-2614-z |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180919 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 1033566837 |a Labeit, Siegfried |m 1033566837:Labeit, Siegfried |d 60000 |d 61600 |e 60000PL1033566837 |e 61600PL1033566837 |k 0/60000/ |k 1/60000/61600/ |p 4 | ||
| 999 | |a KXP-PPN1581095724 |e 3025824212 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"title":[{"title":"FoxO3a suppression and VPS34 activity are essential to anti-atrophic effects of leucine in skeletal muscle","title_sort":"FoxO3a suppression and VPS34 activity are essential to anti-atrophic effects of leucine in skeletal muscle"}],"relHost":[{"pubHistory":["Volume 148, issue 1 (March 1974)-"],"part":{"issue":"2","extent":"14","pages":"381-394","volume":"369","text":"369(2017), 2, Seite 381-394","year":"2017"},"language":["eng"],"title":[{"title_sort":"Cell & tissue research","title":"Cell & tissue research"}],"origin":[{"publisherPlace":"Berlin ; Heidelberg","dateIssuedDisp":"1974-","publisher":"Springer","dateIssuedKey":"1974"}],"recId":"253390516","type":{"bibl":"periodical","media":"Online-Ressource"},"titleAlt":[{"title":"Cell and tissue research"}],"note":["Gesehen am 24.04.25"],"disp":"FoxO3a suppression and VPS34 activity are essential to anti-atrophic effects of leucine in skeletal muscleCell & tissue research","physDesc":[{"extent":"Online-Ressource"}],"id":{"zdb":["1458496-7"],"issn":["1432-0878"],"eki":["253390516"]}}],"note":["Im Titel sind die Ziffern „3“ und \"34\" tiefgestellt","Gesehen am 19.09.2018"],"physDesc":[{"extent":"14 S."}],"id":{"doi":["10.1007/s00441-017-2614-z"],"eki":["1581095724"]},"titleAlt":[{"title":"FoxO 3 a suppression and VPS 34 activity are essential to anti-atrophic effects of leucine in skeletal muscle"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"origin":[{"dateIssuedKey":"2017","dateIssuedDisp":"24 April 2017"}],"recId":"1581095724","person":[{"family":"Baptista","display":"Baptista, Igor L.","role":"aut","given":"Igor L."},{"display":"Labeit, Siegfried","role":"aut","given":"Siegfried","family":"Labeit"}],"name":{"displayForm":["Igor L. Baptista, João G. Silvestre, William J. Silva, Siegfried Labeit, Anselmo S. Moriscot"]}} | ||
| SRT | |a BAPTISTAIGFOXO3ASUPP2420 | ||