Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation
Plasma membrane (PM) blebs are dynamic actin-rich cell protrusions that occur, e.g., during cytokinesis, amoeboid cell motility and cell attachment. Using a targeted siRNA screen against 21 actin nucleation factors, we identify a novel and essential role of the human diaphanous formin DIAPH3 in PM b...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2012
|
| In: |
Cell research
Year: 2011, Jahrgang: 22, Heft: 4, Pages: 728-745 |
| ISSN: | 1748-7838 |
| DOI: | 10.1038/cr.2011.202 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1038/cr.2011.202 Verlag, Volltext: https://www.nature.com/articles/cr2011202 
|
| Verfasserangaben: | Jana Stastna, Xiaoyu Pan, Haicui Wang, Alina Kollmannsperger, Stefan Kutscheidt, Volker Lohmann, Robert Grosse, Oliver T. Fackler |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1581129033 | ||
| 003 | DE-627 | ||
| 005 | 20220815013857.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180920r20122011xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/cr.2011.202 |2 doi | |
| 035 | |a (DE-627)1581129033 | ||
| 035 | |a (DE-576)511129033 | ||
| 035 | |a (DE-599)BSZ511129033 | ||
| 035 | |a (OCoLC)1341018841 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 19 |2 sdnb | ||
| 100 | 1 | |a Št̓astná, Jana |d 1982- |e VerfasserIn |0 (DE-588)1021669229 |0 (DE-627)71582600X |0 (DE-576)362765847 |4 aut | |
| 245 | 1 | 0 | |a Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation |c Jana Stastna, Xiaoyu Pan, Haicui Wang, Alina Kollmannsperger, Stefan Kutscheidt, Volker Lohmann, Robert Grosse, Oliver T. Fackler |
| 264 | 1 | |c 2012 | |
| 300 | |a 18 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Published: 20 December 2011 | ||
| 500 | |a Gesehen am 20.09.2018 | ||
| 520 | |a Plasma membrane (PM) blebs are dynamic actin-rich cell protrusions that occur, e.g., during cytokinesis, amoeboid cell motility and cell attachment. Using a targeted siRNA screen against 21 actin nucleation factors, we identify a novel and essential role of the human diaphanous formin DIAPH3 in PM blebbing during cell adhesion. Suppression of DIAPH3 inhibited blebbing to promote rapid cell spreading involving β1-integrin. Multiple isoforms of DIAPH3 were detected on the mRNA and protein level of which isoforms 3 and 7 were the largest and most abundant isoforms that however did not induce formation of actin-rich protrusions. Rather, PM blebbing specifically involved the low abundance isoform 1 of DIAPH3 and activation of isoform 7 by deletion of the diaphanous-autoregulatory domain caused the formation of filopodia. Dimerization and actin assembly activity were essential for induction of specific cell protrusions by DIAPH3 isoforms 1 and 7. Our data suggest that the N-terminal region comprising the GTPase-binding domain determined the subcellular localization of the formin as well as its protrusion activity between blebs and filopodia. We propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility. | ||
| 534 | |c 2011 | ||
| 700 | 1 | |a Pan, Xiaoyu |e VerfasserIn |0 (DE-588)102668188X |0 (DE-627)727056190 |0 (DE-576)37196766X |4 aut | |
| 700 | 1 | |a Kutscheidt, Stefan |d 1979- |e VerfasserIn |0 (DE-588)1054560269 |0 (DE-627)791604241 |0 (DE-576)41032003X |4 aut | |
| 700 | 1 | |a Lohmann, Volker |e VerfasserIn |0 (DE-588)1060228807 |0 (DE-627)799317136 |0 (DE-576)416317537 |4 aut | |
| 700 | 1 | |a Fackler, Oliver Till |e VerfasserIn |0 (DE-588)1026682444 |0 (DE-627)727057391 |0 (DE-576)178094501 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cell research |d [London] : Macmillan Publishers Limited, part of Springer Nature, 1990 |g 22(2012), 4, Seite 728-745 |h Online-Ressource |w (DE-627)350260435 |w (DE-600)2082402-6 |w (DE-576)306830132 |x 1748-7838 |7 nnas |a Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation |
| 773 | 1 | 8 | |g volume:22 |g year:2012 |g number:4 |g pages:728-745 |g extent:18 |a Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1038/cr.2011.202 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/cr2011202 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180920 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 1026682444 |a Fackler, Oliver Till |m 1026682444:Fackler, Oliver Till |d 910000 |d 911700 |d 700000 |d 718000 |e 910000PF1026682444 |e 911700PF1026682444 |e 700000PF1026682444 |e 718000PF1026682444 |k 0/910000/ |k 1/910000/911700/ |k 0/700000/ |k 1/700000/718000/ |p 8 |y j | ||
| 998 | |g 1060228807 |a Lohmann, Volker |m 1060228807:Lohmann, Volker |d 910000 |d 911700 |d 700000 |d 718000 |e 910000PL1060228807 |e 911700PL1060228807 |e 700000PL1060228807 |e 718000PL1060228807 |k 0/910000/ |k 1/910000/911700/ |k 0/700000/ |k 1/700000/718000/ |p 6 | ||
| 998 | |g 1054560269 |a Kutscheidt, Stefan |m 1054560269:Kutscheidt, Stefan |d 910000 |d 911700 |e 910000PK1054560269 |e 911700PK1054560269 |k 0/910000/ |k 1/910000/911700/ |p 5 | ||
| 998 | |g 102668188X |a Pan, Xiaoyu |m 102668188X:Pan, Xiaoyu |d 910000 |d 911700 |e 910000PP102668188X |e 911700PP102668188X |k 0/910000/ |k 1/910000/911700/ |p 2 | ||
| 998 | |g 1021669229 |a Št̕astná, Jana |m 1021669229:Št̕astná, Jana |d 910000 |d 911700 |e 910000PS1021669229 |e 911700PS1021669229 |k 0/910000/ |k 1/910000/911700/ |p 1 |x j | ||
| 999 | |a KXP-PPN1581129033 |e 3025882794 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"extent":"18 S."}],"title":[{"title":"Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation","title_sort":"Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation"}],"relHost":[{"language":["eng"],"pubHistory":["1.1990 -"],"note":["Gesehen am 21.11.25","China-Zeitschriften-Code: CN31-1568","CAJ control code: XXBY"],"type":{"bibl":"periodical","media":"Online-Ressource"},"recId":"350260435","id":{"eki":["350260435"],"zdb":["2082402-6"],"issn":["1748-7838"]},"name":{"displayForm":["Zhong guo ke xue yuan shang hai sheng ming ke xue yan jiu yuan sheng wu hua xue yu xi bao sheng wu xue yan jiu suo; Zhong guo xi bao sheng wu xue xue hui"]},"origin":[{"dateIssuedKey":"1990","publisherPlace":"[London] ; Shanghai","dateIssuedDisp":"1990-","publisher":"Macmillan Publishers Limited, part of Springer Nature ; Institute of Biochemistry and Cell Biology"}],"disp":"Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formationCell research","title":[{"title_sort":"Cell research","title":"Cell research"}],"physDesc":[{"extent":"Online-Ressource"}],"part":{"extent":"18","pages":"728-745","year":"2012","issue":"4","text":"22(2012), 4, Seite 728-745","volume":"22"}}],"name":{"displayForm":["Jana Stastna, Xiaoyu Pan, Haicui Wang, Alina Kollmannsperger, Stefan Kutscheidt, Volker Lohmann, Robert Grosse, Oliver T. Fackler"]},"origin":[{"dateIssuedDisp":"2012","dateIssuedKey":"2012"}],"recId":"1581129033","id":{"doi":["10.1038/cr.2011.202"],"eki":["1581129033"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"person":[{"display":"Št̓astná, Jana","role":"aut","family":"Št̓astná","given":"Jana"},{"role":"aut","family":"Pan","display":"Pan, Xiaoyu","given":"Xiaoyu"},{"display":"Kutscheidt, Stefan","family":"Kutscheidt","role":"aut","given":"Stefan"},{"role":"aut","family":"Lohmann","display":"Lohmann, Volker","given":"Volker"},{"given":"Oliver Till","display":"Fackler, Oliver Till","family":"Fackler","role":"aut"}],"note":["Published: 20 December 2011","Gesehen am 20.09.2018"],"language":["eng"]} | ||
| SRT | |a STASTNAJANDIFFERINGA2012 | ||