Amino acids for Diels-Alder reactions in living cells
Under tension: A set of genetically encoded unnatural amino acids can be used for biocompatible site-specific labeling of proteins with fluorogenic dyes. The new compounds have norbornene and trans-cyclooctene units that react with tetrazine derivatives in an inverse-electron-demand Diels-Alder cycl...
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| Hauptverfasser: | , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2012 Mar 30
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| In: |
Angewandte Chemie. International edition
Year: 2012, Jahrgang: 51, Heft: 17, Pages: 4166-4170 |
| ISSN: | 1521-3773 |
| DOI: | 10.1002/anie.201108231 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1002/anie.201108231 Verlag, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201108231 
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| Verfasserangaben: | Tilman Plass, Sigrid Milles, Christine Koehler, Jędrzej Szymański, Rainer Mueller, Manfred Wießler, Carsten Schultz, and Edward A. Lemke |
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| 520 | |a Under tension: A set of genetically encoded unnatural amino acids can be used for biocompatible site-specific labeling of proteins with fluorogenic dyes. The new compounds have norbornene and trans-cyclooctene units that react with tetrazine derivatives in an inverse-electron-demand Diels-Alder cycloaddition (left in picture). The technique offers fast labeling that is orthogonal to labeling through azide-cyclooctyne click reaction (right). | ||
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