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Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range

Single molecule observation of fluorescence resonance energy transfer can be used to provide insights into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions wit...

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Hauptverfasser: Milles, Sigrid (VerfasserIn) , Köhler, Christine (VerfasserIn) , Lemke, Edward A. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 13 Jun 2012
In: Molecular BioSystems
Year: 2012, Jahrgang: 8, Heft: 10, Pages: 2531-2534
ISSN:1742-2051
DOI:10.1039/c2mb25135c
Online-Zugang:Verlag, Volltext: http://dx.doi.org/10.1039/c2mb25135c
Verlag, Volltext: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441834/
Volltext
Verfasserangaben:Sigrid Milles, Christine Koehler, Yann Gambin, Ashok A Deniz, and Edward A Lemke
Beschreibung
Zusammenfassung:Single molecule observation of fluorescence resonance energy transfer can be used to provide insights into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsically disordered proteins.
Beschreibung:Gesehen am 21.09.2018
Beschreibung:Online Resource
ISSN:1742-2051
DOI:10.1039/c2mb25135c

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