Bisazide cyanine dyes as fuorogenic probes for bis-cyclooctynylated peptide tags and as fluorogenic cross-linkers of cyclooctynylated proteins
Herein we present the synthesis and fluorogenic characterization of a series of double-quenched bisazide cyanine probes with emission maxima between 565 and 580 nm that can participate in covalent, two-point binding bioorthogonal tagging schemes in combination with bis-cyclooctynylated peptides. Com...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
April 25, 2017
|
| In: |
Bioconjugate chemistry
Year: 2017, Volume: 28, Issue: 5, Pages: 1552-1559 |
| ISSN: | 1520-4812 |
| DOI: | 10.1021/acs.bioconjchem.7b00178 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1021/acs.bioconjchem.7b00178 Verlag, Volltext: https://doi.org/10.1021/acs.bioconjchem.7b00178 
|
| Author Notes: | Orsolya Demeter, Attila Kormos, Christine Koehler, Gábor Mező, Krisztina Németh, Eszter Kozma, Levente B. Takács, Edward A. Lemke, and Péter Kele |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1581248059 | ||
| 003 | DE-627 | ||
| 005 | 20220815015711.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180924s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1021/acs.bioconjchem.7b00178 |2 doi | |
| 035 | |a (DE-627)1581248059 | ||
| 035 | |a (DE-576)511248059 | ||
| 035 | |a (DE-599)BSZ511248059 | ||
| 035 | |a (OCoLC)1341019223 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 30 |2 sdnb | ||
| 100 | 1 | |a Demeter, Orsolya |e VerfasserIn |0 (DE-588)1167735730 |0 (DE-627)1031254951 |0 (DE-576)511246773 |4 aut | |
| 245 | 1 | 0 | |a Bisazide cyanine dyes as fuorogenic probes for bis-cyclooctynylated peptide tags and as fluorogenic cross-linkers of cyclooctynylated proteins |c Orsolya Demeter, Attila Kormos, Christine Koehler, Gábor Mező, Krisztina Németh, Eszter Kozma, Levente B. Takács, Edward A. Lemke, and Péter Kele |
| 264 | 1 | |c April 25, 2017 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 24.09.2018 | ||
| 520 | |a Herein we present the synthesis and fluorogenic characterization of a series of double-quenched bisazide cyanine probes with emission maxima between 565 and 580 nm that can participate in covalent, two-point binding bioorthogonal tagging schemes in combination with bis-cyclooctynylated peptides. Compared to other fluorogenic cyanines, these double-quenched systems showed remarkable fluorescence intensity increase upon formation of cyclic dye-peptide conjugates. Furthermore, we also demonstrated that these bisazides are useful fluorogenic cross-linking platforms that are able to form a covalent linkage between monocyclooctynylated proteins. | ||
| 700 | 1 | |a Köhler, Christine |d 1981- |e VerfasserIn |0 (DE-588)11362803X |0 (DE-627)731770935 |0 (DE-576)376302798 |4 aut | |
| 700 | 1 | |a Lemke, Edward A. |d 1978- |e VerfasserIn |0 (DE-588)131470523 |0 (DE-627)707639824 |0 (DE-576)298499134 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Bioconjugate chemistry |d Columbus, Ohio : American Chemical Society, 1990 |g 28(2017), 5, Seite 1552-1559 |h Online-Ressource |w (DE-627)306655780 |w (DE-600)1500067-9 |w (DE-576)088732371 |x 1520-4812 |7 nnas |a Bisazide cyanine dyes as fuorogenic probes for bis-cyclooctynylated peptide tags and as fluorogenic cross-linkers of cyclooctynylated proteins |
| 773 | 1 | 8 | |g volume:28 |g year:2017 |g number:5 |g pages:1552-1559 |g extent:8 |a Bisazide cyanine dyes as fuorogenic probes for bis-cyclooctynylated peptide tags and as fluorogenic cross-linkers of cyclooctynylated proteins |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1021/acs.bioconjchem.7b00178 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://doi.org/10.1021/acs.bioconjchem.7b00178 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20180924 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 131470523 |a Lemke, Edward A. |m 131470523:Lemke, Edward A. |d 700000 |d 718000 |e 700000PL131470523 |e 718000PL131470523 |k 0/700000/ |k 1/700000/718000/ |p 8 | ||
| 998 | |g 11362803X |a Köhler, Christine |m 11362803X:Köhler, Christine |p 3 | ||
| 999 | |a KXP-PPN1581248059 |e 3026438200 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Orsolya Demeter, Attila Kormos, Christine Koehler, Gábor Mező, Krisztina Németh, Eszter Kozma, Levente B. Takács, Edward A. Lemke, and Péter Kele"]},"person":[{"family":"Demeter","display":"Demeter, Orsolya","role":"aut","given":"Orsolya"},{"display":"Köhler, Christine","role":"aut","given":"Christine","family":"Köhler"},{"display":"Lemke, Edward A.","given":"Edward A.","role":"aut","family":"Lemke"}],"origin":[{"dateIssuedDisp":"April 25, 2017","dateIssuedKey":"2017"}],"recId":"1581248059","type":{"media":"Online-Ressource","bibl":"article-journal"},"id":{"doi":["10.1021/acs.bioconjchem.7b00178"],"eki":["1581248059"]},"physDesc":[{"extent":"8 S."}],"note":["Gesehen am 24.09.2018"],"relHost":[{"pubHistory":["1.1990 -"],"title":[{"title":"Bioconjugate chemistry","title_sort":"Bioconjugate chemistry"}],"part":{"volume":"28","text":"28(2017), 5, Seite 1552-1559","year":"2017","pages":"1552-1559","extent":"8","issue":"5"},"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"disp":"Bisazide cyanine dyes as fuorogenic probes for bis-cyclooctynylated peptide tags and as fluorogenic cross-linkers of cyclooctynylated proteinsBioconjugate chemistry","id":{"zdb":["1500067-9"],"issn":["1520-4812"],"eki":["306655780"]},"note":["Gesehen am 18.11.08"],"type":{"bibl":"periodical","media":"Online-Ressource"},"recId":"306655780","origin":[{"dateIssuedDisp":"1990-","publisher":"American Chemical Society","dateIssuedKey":"1990","publisherPlace":"Columbus, Ohio"}],"name":{"displayForm":["American Chemical Society"]}}],"title":[{"title":"Bisazide cyanine dyes as fuorogenic probes for bis-cyclooctynylated peptide tags and as fluorogenic cross-linkers of cyclooctynylated proteins","title_sort":"Bisazide cyanine dyes as fuorogenic probes for bis-cyclooctynylated peptide tags and as fluorogenic cross-linkers of cyclooctynylated proteins"}],"language":["eng"]} | ||
| SRT | |a DEMETERORSBISAZIDECY2520 | ||