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Mitotic lamin disassembly is triggered by lipid-mediated signaling

Disassembly of the nuclear lamina is a key step during open mitosis in higher eukaryotes. The activity of several kinases, including CDK1 (cyclin-dependent kinase 1) and protein kinase C (PKC), has been shown to trigger mitotic lamin disassembly, yet their precise contributions are unclear. In this...

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Main Authors: Mall, Moritz (Author) , Walter, Thomas (Author) , Gorjánácz, Mátyás (Author) , Davidson, Iain F. (Author) , Ly-Hartig, Thi Bach Nga (Author) , Ellenberg, Jan (Author) , Mattaj, Iain W. (Author)
Format: Article (Journal)
Language:English
Published: 17 September 2012
In: The journal of cell biology
Year: 2012, Volume: 198, Issue: 6, Pages: 981-990
ISSN:1540-8140
DOI:10.1083/jcb.201205103
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1083/jcb.201205103
Verlag, kostenfrei, Volltext: http://jcb.rupress.org/content/198/6/981
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Author Notes:Moritz Mall, Thomas Walter, Mátyás Gorjánácz, Iain F. Davidson, Thi Bach Nga Ly-Hartig, Jan Ellenberg and Iain W. Mattaj
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Summary:Disassembly of the nuclear lamina is a key step during open mitosis in higher eukaryotes. The activity of several kinases, including CDK1 (cyclin-dependent kinase 1) and protein kinase C (PKC), has been shown to trigger mitotic lamin disassembly, yet their precise contributions are unclear. In this study, we develop a quantitative imaging assay to study mitotic lamin B1 disassembly in living cells. We find that CDK1 and PKC act in concert to mediate phosphorylation-dependent lamin B1 disassembly during mitosis. Using ribonucleic acid interference (RNAi), we showed that diacylglycerol (DAG)-dependent PKCs triggered rate-limiting steps of lamin disassembly. RNAi-mediated depletion or chemical inhibition of lipins, enzymes that produce DAG, delayed lamin disassembly to a similar extent as does PKC inhibition/depletion. Furthermore, the delay of lamin B1 disassembly after lipin depletion could be rescued by the addition of DAG. These findings suggest that lipins activate a PKC-dependent pathway during mitotic lamin disassembly and provide evidence for a lipid-mediated mitotic signaling event.
Item Description:Gesehen am 02.10.2018
Physical Description:Online Resource
ISSN:1540-8140
DOI:10.1083/jcb.201205103

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