Mitotic lamin disassembly is triggered by lipid-mediated signaling
Disassembly of the nuclear lamina is a key step during open mitosis in higher eukaryotes. The activity of several kinases, including CDK1 (cyclin-dependent kinase 1) and protein kinase C (PKC), has been shown to trigger mitotic lamin disassembly, yet their precise contributions are unclear. In this...
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| Hauptverfasser: | , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
17 September 2012
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| In: |
The journal of cell biology
Year: 2012, Jahrgang: 198, Heft: 6, Pages: 981-990 |
| ISSN: | 1540-8140 |
| DOI: | 10.1083/jcb.201205103 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1083/jcb.201205103 Verlag, kostenfrei, Volltext: http://jcb.rupress.org/content/198/6/981 
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| Verfasserangaben: | Moritz Mall, Thomas Walter, Mátyás Gorjánácz, Iain F. Davidson, Thi Bach Nga Ly-Hartig, Jan Ellenberg and Iain W. Mattaj |
MARC
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| 520 | |a Disassembly of the nuclear lamina is a key step during open mitosis in higher eukaryotes. The activity of several kinases, including CDK1 (cyclin-dependent kinase 1) and protein kinase C (PKC), has been shown to trigger mitotic lamin disassembly, yet their precise contributions are unclear. In this study, we develop a quantitative imaging assay to study mitotic lamin B1 disassembly in living cells. We find that CDK1 and PKC act in concert to mediate phosphorylation-dependent lamin B1 disassembly during mitosis. Using ribonucleic acid interference (RNAi), we showed that diacylglycerol (DAG)-dependent PKCs triggered rate-limiting steps of lamin disassembly. RNAi-mediated depletion or chemical inhibition of lipins, enzymes that produce DAG, delayed lamin disassembly to a similar extent as does PKC inhibition/depletion. Furthermore, the delay of lamin B1 disassembly after lipin depletion could be rescued by the addition of DAG. These findings suggest that lipins activate a PKC-dependent pathway during mitotic lamin disassembly and provide evidence for a lipid-mediated mitotic signaling event. | ||
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