Ligand-modulated folding of the full-length adenine riboswitch probed by NMR and single-molecule FRET spectroscopy
The full-length translation-regulating add adenine riboswitch (Asw) from Vibrio vulnificus has a more complex conformational space than its isolated aptamer domain. In addition to the predicted apo (apoA) and holo conformation that feature the conserved three-way junctional purine riboswitch aptamer...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2017 May 19
|
| In: |
Nucleic acids symposium series
Year: 2017, Jahrgang: 45, Heft: 9, Pages: 5512-5522 |
| ISSN: | 1746-8272 |
| DOI: | 10.1093/nar/gkx110 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1093/nar/gkx110 Verlag, kostenfrei, Volltext: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605240/ 
|
| Verfasserangaben: | Sven Warhaut, Klara Rebecca Mertinkus, Philipp Höllthaler, Boris Fürtig, Mike Heilemann, Martin Hengesbach and Harald Schwalbe |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 158161957X | ||
| 003 | DE-627 | ||
| 005 | 20220815024347.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 181008s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1093/nar/gkx110 |2 doi | |
| 035 | |a (DE-627)158161957X | ||
| 035 | |a (DE-576)51161957X | ||
| 035 | |a (DE-599)BSZ51161957X | ||
| 035 | |a (OCoLC)1341019535 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Warhaut, Sven |d 1989- |e VerfasserIn |0 (DE-588)1136330410 |0 (DE-627)892645636 |0 (DE-576)490435475 |4 aut | |
| 245 | 1 | 0 | |a Ligand-modulated folding of the full-length adenine riboswitch probed by NMR and single-molecule FRET spectroscopy |c Sven Warhaut, Klara Rebecca Mertinkus, Philipp Höllthaler, Boris Fürtig, Mike Heilemann, Martin Hengesbach and Harald Schwalbe |
| 264 | 1 | |c 2017 May 19 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.10.2018 | ||
| 500 | |a Published online: 15 February 2017 | ||
| 520 | |a The full-length translation-regulating add adenine riboswitch (Asw) from Vibrio vulnificus has a more complex conformational space than its isolated aptamer domain. In addition to the predicted apo (apoA) and holo conformation that feature the conserved three-way junctional purine riboswitch aptamer, it adopts a second apo (apoB) conformation with a fundamentally different secondary structure. Here, we characterized the ligand-dependent conformational dynamics of the full-length add Asw by NMR and by single-molecule FRET (smFRET) spectroscopy. Both methods revealed an adenine-induced secondary structure switch from the apoB-form to the apoA-form that involves no tertiary structural interactions between aptamer and expression platform. This strongly suggests that the add Asw triggers translation by capturing the apoA-form secondary structure in the holo state. Intriguingly, NMR indicated a homogenous, docked aptamer kissing loop fold for apoA and holo, while smFRET showed persistent aptamer kissing loop docking dynamics between comparably stable, undocked and docked substates of the apoA and the holo conformation. Unraveling the folding of large junctional riboswitches thus requires the integration of complementary solution structural techniques such as NMR and smFRET. | ||
| 700 | 1 | |a Heilemann, Mike |e VerfasserIn |0 (DE-588)1096549891 |0 (DE-627)856929999 |0 (DE-576)33572535X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nucleic acids symposium series |d Oxford : Oxford Univ. Press, 1999 |g 45(2017), 9, Seite 5512-5522 |h Online-Ressource |w (DE-627)501075720 |w (DE-600)2205588-5 |w (DE-576)306832615 |x 1746-8272 |7 nnas |a Ligand-modulated folding of the full-length adenine riboswitch probed by NMR and single-molecule FRET spectroscopy |
| 773 | 1 | 8 | |g volume:45 |g year:2017 |g number:9 |g pages:5512-5522 |g extent:11 |a Ligand-modulated folding of the full-length adenine riboswitch probed by NMR and single-molecule FRET spectroscopy |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1093/nar/gkx110 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605240/ |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20181008 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 1096549891 |a Heilemann, Mike |m 1096549891:Heilemann, Mike |d 700000 |d 716000 |d 700000 |d 718000 |e 700000PH1096549891 |e 716000PH1096549891 |e 700000PH1096549891 |e 718000PH1096549891 |k 0/700000/ |k 1/700000/716000/ |k 0/700000/ |k 1/700000/718000/ |p 5 | ||
| 999 | |a KXP-PPN158161957X |e 3028031027 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Sven Warhaut, Klara Rebecca Mertinkus, Philipp Höllthaler, Boris Fürtig, Mike Heilemann, Martin Hengesbach and Harald Schwalbe"]},"id":{"doi":["10.1093/nar/gkx110"],"eki":["158161957X"]},"origin":[{"dateIssuedDisp":"2017 May 19","dateIssuedKey":"2017"}],"relHost":[{"origin":[{"dateIssuedDisp":"[1999?-2009]","publisher":"Oxford Univ. Press","publisherPlace":"Oxford"}],"id":{"zdb":["2205588-5"],"eki":["501075720"],"issn":["1746-8272"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"Nucleic acids symposium series","title_sort":"Nucleic acids symposium series"}],"note":["Fortsetzung der Druck-Ausgabe"],"disp":"Ligand-modulated folding of the full-length adenine riboswitch probed by NMR and single-molecule FRET spectroscopyNucleic acids symposium series","type":{"bibl":"periodical","media":"Online-Ressource"},"language":["eng"],"recId":"501075720","pubHistory":["Volume 42, issue 1 (01 November 1999) [?]-volume 53, issue 1 (September/October 2009) ; damit Erscheinen eingestellt"],"titleAlt":[{"title":"Nucleic acids research / Symposium series / Nucleic acids symposium series"}],"part":{"extent":"11","volume":"45","text":"45(2017), 9, Seite 5512-5522","issue":"9","pages":"5512-5522","year":"2017"}}],"physDesc":[{"extent":"11 S."}],"person":[{"role":"aut","roleDisplay":"VerfasserIn","display":"Warhaut, Sven","given":"Sven","family":"Warhaut"},{"role":"aut","display":"Heilemann, Mike","roleDisplay":"VerfasserIn","given":"Mike","family":"Heilemann"}],"title":[{"title":"Ligand-modulated folding of the full-length adenine riboswitch probed by NMR and single-molecule FRET spectroscopy","title_sort":"Ligand-modulated folding of the full-length adenine riboswitch probed by NMR and single-molecule FRET spectroscopy"}],"language":["eng"],"recId":"158161957X","type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 08.10.2018","Published online: 15 February 2017"]} | ||
| SRT | |a WARHAUTSVELIGANDMODU2017 | ||