Architecture of the yeast elongator complex
The highly conserved eukaryotic Elongator complex performs specific chemical modifications on wobble base uridines of tRNAs, which are essential for proteome stability and homeostasis. The complex is formed by six individual subunits (Elp1‐6) that are all equally important for its tRNA modification...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
01 February 2017
|
| In: |
EMBO reports
Year: 2016, Jahrgang: 18, Heft: 2, Pages: 264-279 |
| ISSN: | 1469-3178 |
| DOI: | 10.15252/embr.201643353 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.15252/embr.201643353 Verlag, Volltext: http://embor.embopress.org/content/18/2/264 
|
| Verfasserangaben: | Maria I. Dauden, Jan Kosinski, Olga Kolaj‐Robin, Ambroise Desfosses, Alessandro Ori, Celine Faux, Niklas A. Hoffmann, Osita F. Onuma, Karin D. Breunig, Martin Beck, Carsten Sachse, Bertrand Séraphin, Sebastian Glatt, Christoph W. Müller |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1581636849 | ||
| 003 | DE-627 | ||
| 005 | 20220815024705.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 181008r20172016xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.15252/embr.201643353 |2 doi | |
| 035 | |a (DE-627)1581636849 | ||
| 035 | |a (DE-576)511636849 | ||
| 035 | |a (DE-599)BSZ511636849 | ||
| 035 | |a (OCoLC)1341019727 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Dauden, Maria I. |e VerfasserIn |0 (DE-588)1168610494 |0 (DE-627)1032377526 |0 (DE-576)511637756 |4 aut | |
| 245 | 1 | 0 | |a Architecture of the yeast elongator complex |c Maria I. Dauden, Jan Kosinski, Olga Kolaj‐Robin, Ambroise Desfosses, Alessandro Ori, Celine Faux, Niklas A. Hoffmann, Osita F. Onuma, Karin D. Breunig, Martin Beck, Carsten Sachse, Bertrand Séraphin, Sebastian Glatt, Christoph W. Müller |
| 264 | 1 | |c 01 February 2017 | |
| 300 | |a 16 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.10.2018 | ||
| 500 | |a Published online 14.12.2016 | ||
| 520 | |a The highly conserved eukaryotic Elongator complex performs specific chemical modifications on wobble base uridines of tRNAs, which are essential for proteome stability and homeostasis. The complex is formed by six individual subunits (Elp1‐6) that are all equally important for its tRNA modification activity. However, its overall architecture and the detailed reaction mechanism remain elusive. Here, we report the structures of the fully assembled yeast Elongator and the Elp123 sub‐complex solved by an integrative structure determination approach showing that two copies of the Elp1, Elp2, and Elp3 subunits form a two‐lobed scaffold, which binds Elp456 asymmetrically. Our topological models are consistent with previous studies on individual subunits and further validated by complementary biochemical analyses. Our study provides a structural framework on how the tRNA modification activity is carried out by Elongator. | ||
| 534 | |c 2016 | ||
| 650 | 4 | |a electron microscopy | |
| 650 | 4 | |a Elongator | |
| 650 | 4 | |a Saccharomyces cerevisiae | |
| 650 | 4 | |a tRNA modification | |
| 650 | 4 | |a yeast | |
| 700 | 1 | |a Beck, Martin |d 1977- |e VerfasserIn |0 (DE-588)131458647 |0 (DE-627)509456634 |0 (DE-576)29849101X |4 aut | |
| 700 | 1 | |a Müller, Christoph W. |d 1960- |e VerfasserIn |0 (DE-588)1135746710 |0 (DE-627)890784477 |0 (DE-576)167743481 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a European Molecular Biology Organization |t EMBO reports |d [London] : Nature Publishing Group UK, 2000 |g 18(2017), 2, Seite 264-279 |h Online-Ressource |w (DE-627)320645622 |w (DE-600)2025376-X |w (DE-576)095092153 |x 1469-3178 |7 nnas |
| 773 | 1 | 8 | |g volume:18 |g year:2017 |g number:2 |g pages:264-279 |g extent:16 |a Architecture of the yeast elongator complex |
| 856 | 4 | 0 | |u http://dx.doi.org/10.15252/embr.201643353 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://embor.embopress.org/content/18/2/264 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20181008 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 1135746710 |a Müller, Christoph W. |m 1135746710:Müller, Christoph W. |d 700000 |d 718000 |e 700000PM1135746710 |e 718000PM1135746710 |k 0/700000/ |k 1/700000/718000/ |p 14 |y j | ||
| 998 | |g 131458647 |a Beck, Martin |m 131458647:Beck, Martin |d 700000 |d 718000 |e 700000PB131458647 |e 718000PB131458647 |k 0/700000/ |k 1/700000/718000/ |p 10 | ||
| 999 | |a KXP-PPN1581636849 |e 3028056747 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 08.10.2018"," Published online 14.12.2016"],"physDesc":[{"extent":"16 S."}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"note":["Fortsetzung der Druck-Ausgabe","Gesehen am 11.03.25"],"language":["eng"],"disp":"European Molecular Biology OrganizationEMBO reports","corporate":[{"role":"aut","display":"European Molecular Biology Organization","roleDisplay":"VerfasserIn"}],"origin":[{"publisher":"Nature Publishing Group UK ; EMBO Press ; Oxford Univ. Press ; Nature Publishing Group ; Wiley","dateIssuedDisp":"[2000]-","publisherPlace":"[London] ; Heidelberg ; Oxford [u.a.] ; London [u.a.] ; Hoboken, NJ [u.a.]"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"part":{"volume":"18","text":"18(2017), 2, Seite 264-279","extent":"16","issue":"2","year":"2017","pages":"264-279"},"title":[{"title_sort":"EMBO reports","title":"EMBO reports"}],"id":{"doi":["10.1002/(ISSN)1469-3178"],"issn":["1469-3178"],"zdb":["2025376-X"],"eki":["320645622"]},"recId":"320645622","name":{"displayForm":["European Molecular Biology Organization"]},"pubHistory":["1.2000 -"]}],"language":["eng"],"origin":[{"dateIssuedKey":"2017","dateIssuedDisp":"01 February 2017"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"person":[{"role":"aut","roleDisplay":"VerfasserIn","given":"Maria I.","display":"Dauden, Maria I.","family":"Dauden"},{"role":"aut","family":"Beck","roleDisplay":"VerfasserIn","given":"Martin","display":"Beck, Martin"},{"role":"aut","family":"Müller","roleDisplay":"VerfasserIn","given":"Christoph W.","display":"Müller, Christoph W."}],"title":[{"title":"Architecture of the yeast elongator complex","title_sort":"Architecture of the yeast elongator complex"}],"id":{"eki":["1581636849"],"doi":["10.15252/embr.201643353"]},"recId":"1581636849","name":{"displayForm":["Maria I. Dauden, Jan Kosinski, Olga Kolaj‐Robin, Ambroise Desfosses, Alessandro Ori, Celine Faux, Niklas A. Hoffmann, Osita F. Onuma, Karin D. Breunig, Martin Beck, Carsten Sachse, Bertrand Séraphin, Sebastian Glatt, Christoph W. Müller"]}} | ||
| SRT | |a DAUDENMARIARCHITECTU0120 | ||