Human bone marrow mesenchymal stem cells for retinal vascular injury
Purpose: To examine the potential of intravitreally implanted human bone marrow-derived mesenchymal stem cells (BMSCs) to affect vascular repair and the blood-retina barrier in mice and rats with oxygen-induced retinopathy, diabetic retinopathy or retinal ischaemia-reperfusion damage. Methods: Three...
Gespeichert in:
| Hauptverfasser: | , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2017
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| In: |
Acta ophthalmologica
Year: 2016, Jahrgang: 95, Heft: 6, Pages: e453-e461 |
| ISSN: | 1755-3768 |
| DOI: | 10.1111/aos.13154 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1111/aos.13154 Verlag, Volltext: http://onlinelibrary.wiley.com/doi/abs/10.1111/aos.13154 
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| Verfasserangaben: | Jin-Da Wang, Ying An, Jing-Shang Zhang, Xiu-Hua Wan, Jost B. Jonas, Liang Xu and Wei Zhang |
| Zusammenfassung: | Purpose: To examine the potential of intravitreally implanted human bone marrow-derived mesenchymal stem cells (BMSCs) to affect vascular repair and the blood-retina barrier in mice and rats with oxygen-induced retinopathy, diabetic retinopathy or retinal ischaemia-reperfusion damage. Methods: Three study groups (oxygen-induced retinopathy group: 18 C57BL/6J mice; diabetic retinopathy group: 15 rats; retinal ischaemia-reperfusion model: 18 rats) received BMSCs injected intravitreally. Control groups (oxygen-induced retinopathy group: 12 C57BL/6J mice; diabetic retinopathy group: 15 rats; retinal ischaemia-reperfusion model: 18 rats) received an intravitreal injection of phosphate-buffered saline. We applied immunohistological techniques to measure retinal vascularization, spectroscopic measurements of intraretinally extravasated fluorescein-conjugated dextran to quantify the blood-retina barrier breakdown, and histomorphometry to assess retinal thickness and retinal ganglion cell count. Results: In the oxygen-induced retinopathy model, the study group with intravitreally injected BMSCs as compared with the control group showed a significantly (p = 0.001) smaller area of retinal neovascularization. In the diabetic retinopathy model, study group and control group did not differ significantly in the amount of intraretinally extravasated dextran. In the retinal ischaemia-reperfusion model, on the 7th day after retina injury, the retina was significantly thicker in the study group than in the control group (p = 0.02), with no significant difference in the retinal ganglion cell count (p = 0.36). Conclusions: Intravitreally implanted human BMSCs were associated with a reduced retinal neovascularization in the oxygen-induced retinopathy model and with a potentially cell preserving effect in the retinal ischaemia-reperfusion model. Intravitreal BMSCs may be of potential interest for the therapy of retinal vascular disorders. |
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| Beschreibung: | First published: 03 November 2016 Gesehen am 08.10.2018 |
| Beschreibung: | Online Resource |
| ISSN: | 1755-3768 |
| DOI: | 10.1111/aos.13154 |