EGFR T790M mutation testing of non-small cell lung cancer tissue and blood samples artificially spiked with circulating cell-free tumor DNA: results of a round robin trial
The European Commision (EC) recently approved osimertinib for the treatment of adult patients with locally advanced or metastatic non-small-cell lung cancer (NSCLC) harboring EGFR T790M mutations. Besides tissue-based testing, blood samples containing cell-free circulating tumor DNA (ctDNA) can be u...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
8 September 2017
|
| In: |
Virchows Archiv
Year: 2017, Jahrgang: 471, Heft: 4, Pages: 509-520 |
| ISSN: | 1432-2307 |
| DOI: | 10.1007/s00428-017-2226-8 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1007/s00428-017-2226-8 Verlag, Volltext: https://doi.org/10.1007/s00428-017-2226-8 
|
| Verfasserangaben: | Jana Fassunke, Michaela Angelika Ihle, Dido Lenze, Annika Lehmann, Michael Hummel, Claudia Vollbrecht, Roland Penzel, Anna-Lena Volckmar, Albrecht Stenzinger, Volker Endris, Andreas Jung, Ulrich Lehmann, Silke Zeugner, Gustavo Baretton, Hans Kreipe, Peter Schirmacher, Thomas Kirchner, Manfred Dietel, Reinhard Büttner, Sabine Merkelbach-Bruse |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1581650574 | ||
| 003 | DE-627 | ||
| 005 | 20220815024950.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 181008s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00428-017-2226-8 |2 doi | |
| 035 | |a (DE-627)1581650574 | ||
| 035 | |a (DE-576)511650574 | ||
| 035 | |a (DE-599)BSZ511650574 | ||
| 035 | |a (OCoLC)1341019701 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Fassunke, Jana |d 1976- |e VerfasserIn |0 (DE-588)135915473 |0 (DE-627)574408649 |0 (DE-576)30071775X |4 aut | |
| 245 | 1 | 0 | |a EGFR T790M mutation testing of non-small cell lung cancer tissue and blood samples artificially spiked with circulating cell-free tumor DNA |b results of a round robin trial |c Jana Fassunke, Michaela Angelika Ihle, Dido Lenze, Annika Lehmann, Michael Hummel, Claudia Vollbrecht, Roland Penzel, Anna-Lena Volckmar, Albrecht Stenzinger, Volker Endris, Andreas Jung, Ulrich Lehmann, Silke Zeugner, Gustavo Baretton, Hans Kreipe, Peter Schirmacher, Thomas Kirchner, Manfred Dietel, Reinhard Büttner, Sabine Merkelbach-Bruse |
| 264 | 1 | |c 8 September 2017 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.10.2018 | ||
| 500 | |a Im Titel ist "EGFR" kursiv geschrieben | ||
| 520 | |a The European Commision (EC) recently approved osimertinib for the treatment of adult patients with locally advanced or metastatic non-small-cell lung cancer (NSCLC) harboring EGFR T790M mutations. Besides tissue-based testing, blood samples containing cell-free circulating tumor DNA (ctDNA) can be used to interrogate T790M status. Herein, we describe the conditions and results of a round robin trial (RRT) for T790M mutation testing in NSCLC tissue specimens and peripheral blood samples spiked with cell line DNA mimicking tumor-derived ctDNA. The underlying objectives of this two-staged external quality assessment (EQA) approach were (a) to evaluate the accuracy of T790M mutations testing across multiple centers and (b) to investigate if a liquid biopsy-based testing for T790M mutations in spiked blood samples is feasible in routine diagnostic. Based on a successfully completed internal phase I RRT, an open RRT for EGFR T790M mutation testing in tumor tissue and blood samples was initiated. In total, 48 pathology centers participated in the EQA. Of these, 47 (97.9%) centers submitted their analyses within the pre-defined time frame and 44 (tissue), respectively, 40 (plasma) successfully passed the test. The overall success rates in the RRT phase II were 91.7% (tissue) and 83.3% (blood), respectively. Thirty-eight out of 48 participants (79.2%) successfully passed both parts of the RRT. The RRT for blood-based EGFR testing initiated in Germany is, to the best of our knowledge, the first of his kind in Europe. In summary, our results demonstrate that blood-based genotyping for EGFR resistance mutations can be successfully integrated in routine molecular diagnostics complementing the array of molecular methods already available at pathology centers in Germany. | ||
| 650 | 4 | |a Blood and plasma samples | |
| 650 | 4 | |a DNA sequencing | |
| 650 | 4 | |a EGFR | |
| 650 | 4 | |a FFPE | |
| 650 | 4 | |a Liquid biopsy | |
| 650 | 4 | |a NSCLC | |
| 650 | 4 | |a Osimertinib | |
| 650 | 4 | |a T790M mutation | |
| 650 | 4 | |a Tumor tissue | |
| 700 | 1 | |a Penzel, Roland |e VerfasserIn |0 (DE-588)102044021X |0 (DE-627)691221316 |0 (DE-576)360421083 |4 aut | |
| 700 | 1 | |a Volckmar, Anna-Lena |d 1984- |e VerfasserIn |0 (DE-588)173874916 |0 (DE-627)698776666 |0 (DE-576)134713478 |4 aut | |
| 700 | 1 | |a Stenzinger, Albrecht |e VerfasserIn |0 (DE-588)139606106 |0 (DE-627)703395238 |0 (DE-576)312432755 |4 aut | |
| 700 | 1 | |a Endris, Volker |e VerfasserIn |0 (DE-588)1063753686 |0 (DE-627)812590961 |0 (DE-576)422898198 |4 aut | |
| 700 | 1 | |a Schirmacher, Peter |d 1961- |e VerfasserIn |0 (DE-588)1020440112 |0 (DE-627)691221197 |0 (DE-576)360427448 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Virchows Archiv |d Berlin : Springer, 1847 |g 471(2017), 4, Seite 509-520 |h Online-Ressource |w (DE-627)254910645 |w (DE-600)1463276-7 |w (DE-576)074754416 |x 1432-2307 |7 nnas |a EGFR T790M mutation testing of non-small cell lung cancer tissue and blood samples artificially spiked with circulating cell-free tumor DNA results of a round robin trial |
| 773 | 1 | 8 | |g volume:471 |g year:2017 |g number:4 |g pages:509-520 |g extent:12 |a EGFR T790M mutation testing of non-small cell lung cancer tissue and blood samples artificially spiked with circulating cell-free tumor DNA results of a round robin trial |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00428-017-2226-8 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00428-017-2226-8 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20181008 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 1020440112 |a Schirmacher, Peter |m 1020440112:Schirmacher, Peter |d 910000 |d 912000 |d 700000 |d 718000 |e 910000PS1020440112 |e 912000PS1020440112 |e 700000PS1020440112 |e 718000PS1020440112 |k 0/910000/ |k 1/910000/912000/ |k 0/700000/ |k 1/700000/718000/ |p 16 | ||
| 998 | |g 1063753686 |a Endris, Volker |m 1063753686:Endris, Volker |d 910000 |d 912000 |e 910000PE1063753686 |e 912000PE1063753686 |k 0/910000/ |k 1/910000/912000/ |p 10 | ||
| 998 | |g 139606106 |a Stenzinger, Albrecht |m 139606106:Stenzinger, Albrecht |d 910000 |d 912000 |e 910000PS139606106 |e 912000PS139606106 |k 0/910000/ |k 1/910000/912000/ |p 9 | ||
| 998 | |g 173874916 |a Volckmar, Anna-Lena |m 173874916:Volckmar, Anna-Lena |d 910000 |d 912000 |e 910000PV173874916 |e 912000PV173874916 |k 0/910000/ |k 1/910000/912000/ | ||
| 998 | |g 102044021X |a Penzel, Roland |m 102044021X:Penzel, Roland |d 910000 |d 912000 |e 910000PP102044021X |e 912000PP102044021X |k 0/910000/ |k 1/910000/912000/ |p 7 | ||
| 999 | |a KXP-PPN1581650574 |e 3028186942 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"type":{"media":"Online-Ressource","bibl":"article-journal"},"title":[{"title":"EGFR T790M mutation testing of non-small cell lung cancer tissue and blood samples artificially spiked with circulating cell-free tumor DNA","subtitle":"results of a round robin trial","title_sort":"EGFR T790M mutation testing of non-small cell lung cancer tissue and blood samples artificially spiked with circulating cell-free tumor DNA"}],"id":{"doi":["10.1007/s00428-017-2226-8"],"eki":["1581650574"]},"physDesc":[{"extent":"12 S."}],"relHost":[{"title":[{"title_sort":"Virchows Archiv","subtitle":"official journal of the European Society of Pathology","title":"Virchows Archiv"}],"physDesc":[{"extent":"Online-Ressource"}],"id":{"zdb":["1463276-7"],"issn":["1432-2307"],"eki":["254910645"]},"origin":[{"publisherPlace":"Berlin ; Heidelberg","dateIssuedKey":"1847","dateIssuedDisp":"1847-","publisher":"Springer"}],"recId":"254910645","disp":"EGFR T790M mutation testing of non-small cell lung cancer tissue and blood samples artificially spiked with circulating cell-free tumor DNA results of a round robin trialVirchows Archiv","type":{"bibl":"periodical","media":"Online-Ressource"},"corporate":[{"role":"isb","display":"European Society of Pathology"}],"titleAlt":[{"title":"Archiv für pathologische Anatomie und Physiologie und für klinische Medicin"},{"title":"Virchows Archiv für pathologische Anatomie und Physiologie und für klinische Medizin"},{"title":"Virchows Archiv / A"}],"language":["eng"],"part":{"extent":"12","pages":"509-520","issue":"4","text":"471(2017), 4, Seite 509-520","year":"2017","volume":"471"},"note":["Gesehen am 06.12.05"],"pubHistory":["1.1847 -"]}],"origin":[{"dateIssuedKey":"2017","dateIssuedDisp":"8 September 2017"}],"language":["eng"],"name":{"displayForm":["Jana Fassunke, Michaela Angelika Ihle, Dido Lenze, Annika Lehmann, Michael Hummel, Claudia Vollbrecht, Roland Penzel, Anna-Lena Volckmar, Albrecht Stenzinger, Volker Endris, Andreas Jung, Ulrich Lehmann, Silke Zeugner, Gustavo Baretton, Hans Kreipe, Peter Schirmacher, Thomas Kirchner, Manfred Dietel, Reinhard Büttner, Sabine Merkelbach-Bruse"]},"recId":"1581650574","person":[{"family":"Fassunke","role":"aut","given":"Jana","display":"Fassunke, Jana"},{"family":"Penzel","role":"aut","given":"Roland","display":"Penzel, Roland"},{"display":"Volckmar, Anna-Lena","given":"Anna-Lena","role":"aut","family":"Volckmar"},{"role":"aut","family":"Stenzinger","given":"Albrecht","display":"Stenzinger, Albrecht"},{"display":"Endris, Volker","given":"Volker","family":"Endris","role":"aut"},{"role":"aut","family":"Schirmacher","display":"Schirmacher, Peter","given":"Peter"}],"note":["Gesehen am 08.10.2018","Im Titel ist \"EGFR\" kursiv geschrieben"]} | ||
| SRT | |a FASSUNKEJAEGFRT790MM8201 | ||